• Product name
    Anti-VEGF 165A antibody [6B7]
    See all VEGF 165A primary antibodies
  • Description
    Mouse monoclonal [6B7] to VEGF 165A
  • Host species
  • Specificity
    This antibody reacts with native and denatured human and mouse VEGF. The immunogen is Human Vascular Endothelial Growth Factor 165A, but this has high homology with multiple VEGF isoforms, therefore it should recognise these other isoforms too.
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Full length protein corresponding to Human VEGF 165A. Human Vascular Endothelial Growth Factor 165A.
    Database link: P15692-4

  • Positive control
    • VEGF protein



Our Abpromise guarantee covers the use of ab69479 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).
IP Use a concentration of 2 µg/ml.


  • Function
    Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth.
  • Tissue specificity
    Isoform VEGF189, isoform VEGF165 and isoform VEGF121 are widely expressed. Isoform VEGF206 and isoform VEGF145 are not widely expressed.
  • Involvement in disease
    Defects in VEGFA are a cause of susceptibility to microvascular complications of diabetes type 1 (MVCD1) [MIM:603933]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis.
  • Sequence similarities
    Belongs to the PDGF/VEGF growth factor family.
  • Cellular localization
    Secreted. VEGF121 is acidic and freely secreted. VEGF165 is more basic, has heparin-binding properties and, although a signicant proportion remains cell-associated, most is freely secreted. VEGF189 is very basic, it is cell-associated after secretion and is bound avidly by heparin and the extracellular matrix, although it may be released as a soluble form by heparin, heparinase or plasmin.
  • Information by UniProt
  • Database links
  • Alternative names
    • glioma-derived endothelial cell mitogen antibody
    • MGC70609 antibody
    • MVCD1 antibody
    • Vascular endothelial growth factor A antibody
    • Vascular Endothelial Growth Factor antibody
    • Vascular permeability factor antibody
    • VEGF antibody
    • VEGF-A antibody
    • VEGF165 antibody
    • Vegfa antibody
    • VEGFA_HUMAN antibody
    • VPF antibody
    see all


  • Anti-VEGF 165A antibody [6B7] (ab69479) + VEGF at 0.1 µg

    Predicted band size: 23 kDa
    Observed band size: 23 kDa

  • Lane 1- 1µg VEGF immunoprecipitated with 2µg antibody. Lane 2- HUVEC lysate immunoprecipitated with 2µg antibody.


This product has been referenced in:
  • Zhou Y  et al. Prostaglandin E2 reduces swine myocardial ischemia reperfusion injury via increased endothelial nitric oxide synthase and vascular endothelial growth factor expression levels. Biomed Rep 6:188-194 (2017). IHC-P . Read more (PubMed: 28357071) »
  • Zhou W  et al. MicroRNA-138 inhibits hypoxia-induced proliferation of endothelial progenitor cells via inhibition of HIF-1a-mediated MAPK and AKT signaling. Exp Ther Med 13:1017-1024 (2017). Read more (PubMed: 28450935) »

See all 9 Publications for this product

Customer reviews and Q&As

Expiration date: 07/12/2012

I am very pleased to hear you would like to accept our offer and test ab69479 in ICC/IF. This code will give you: 1 free [PRIMARY ANTIBODY/PROTEIN] OR VALUE OFF OF ORDER before the e...

Read More

Thank you for contacting us. As you mention, we do have a number of related products available. The SEMA3F products we have in our catalog (such as ab39956) is a rabbit-polyclonal antibody against Semaphorin 3F. It is guaranteed to work with mo...

Read More

The accesion number for the VEGF-165A protein is: BAA78418. You can find the NCBI page for more information about this protein at the following link: http://www.ncbi.nlm.nih.gov/protein/4996351.


Sign up