Overview

  • Product name

  • Description

    Rabbit polyclonal to VEGF 165A
  • Host species

    Rabbit
  • Specificity

    ab106580 will recognize both native and recombinant Human VEGF 165A in cell supernatants and certain body fluids. The immunogen is full length recombinant human VEGF-165 protein, but this has high homology with multiple VEGF isoforms, therefore it should recognise these other isoforms too.
  • Tested applications

    Suitable for: WB, ELISAmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant full length protein corresponding to Human VEGF 165A.
    Database link: P15692-4

  • Positive control

    • Recombinant Human VEGF 165A

Properties

Applications

Our Abpromise guarantee covers the use of ab106580 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).
ELISA 1/10000.

Target

  • Function

    Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth.
  • Tissue specificity

    Isoform VEGF189, isoform VEGF165 and isoform VEGF121 are widely expressed. Isoform VEGF206 and isoform VEGF145 are not widely expressed.
  • Involvement in disease

    Defects in VEGFA are a cause of susceptibility to microvascular complications of diabetes type 1 (MVCD1) [MIM:603933]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis.
  • Sequence similarities

    Belongs to the PDGF/VEGF growth factor family.
  • Cellular localization

    Secreted. VEGF121 is acidic and freely secreted. VEGF165 is more basic, has heparin-binding properties and, although a signicant proportion remains cell-associated, most is freely secreted. VEGF189 is very basic, it is cell-associated after secretion and is bound avidly by heparin and the extracellular matrix, although it may be released as a soluble form by heparin, heparinase or plasmin.
  • Information by UniProt
  • Database links

  • Alternative names

    • glioma-derived endothelial cell mitogen antibody
    • MGC70609 antibody
    • MVCD1 antibody
    • Vascular endothelial growth factor A antibody
    • Vascular Endothelial Growth Factor antibody
    • Vascular permeability factor antibody
    • VEGF antibody
    • VEGF-A antibody
    • VEGF165 antibody
    • Vegfa antibody
    • VEGFA_HUMAN antibody
    • VPF antibody
    see all

Images

  • Anti-VEGF 165A antibody (ab106580) at 1/1000 dilution + Recombinant Human VEGF 165A

    Secondary
    Peroxidase conjugated anti-Rabbit IgG at 1/40000 dilution

    Predicted band size: 22 kDa

References

ab106580 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Abreviews
Application
ELISA
Sample
Human Serum (y79 cell supernatant)
Specification
y79 cell supernatant
Blocking step
Superblock as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: 25°C
Type
Sandwich (Capture)

Abcam user community

Verified customer

Submitted Nov 01 2018

Answer

I can confirm that the immunogen is full length recombinant human VEGF-165 protein and ab106580 is a polyclonal antibody. We therefore assume that there are multiple binding sites. Unfortunately with polyclonal antibodies this can not be further mapped.


We have not specifically tested if this antibody binds to the vegfA165b isoform, but as the only difference between vegfA165a and vegfA165b is aa227-232: CDKPRR  SLTRKD (according to Uniprot) it is very likely that ab106580 binds to vegfA165b as well.

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