Overview

  • Product name
    Anti-VEGF Receptor 1 antibody
    See all VEGF Receptor 1 primary antibodies
  • Description
    Rabbit polyclonal to VEGF Receptor 1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, Electron Microscopy, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Pig
  • Immunogen

    Synthetic peptide within Human VEGF Receptor 1 aa 1300 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: P17948

  • Positive control
    • IHC-P: Mouse lung tissue; human angiosarcoma tissue. ICC/IF: A431 cell line
  • General notes


    VEGFR 1 is a cell membrane receptor kinase. It has a high affinity receptor for vascular endothelial growth factor and is putatively involved in the growth of endothelial cells and angiogenesis.

Properties

Applications

Our Abpromise guarantee covers the use of ab2350 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Electron Microscopy 1/100. PubMed: 18647955
WB Use at an assay dependent concentration. PubMed: 21912702
ICC/IF Use a concentration of 5 µg/ml.

Target

  • Function
    Receptor for VEGF, VEGFB and PGF. Has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. Isoform SFlt1 may have an inhibitory role in angiogenesis.
  • Tissue specificity
    Mostly in normal lung, but also in placenta, liver, kidney, heart and brain tissues. Specifically expressed in most of the vascular endothelial cells, and also expressed in peripheral blood monocytes. Isoform sFlt1 is strongly expressed in placenta.
  • Sequence similarities
    Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
    Contains 7 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.
  • Cellular localization
    Secreted and Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • EC 2.7.10.1 antibody
    • FLT 1 antibody
    • FLT antibody
    • Flt-1 antibody
    • FLT1 antibody
    • Fms like tyrosine kinase 1 antibody
    • Fms related tyrosine kinase 1 antibody
    • Fms related tyrosine kinase 1 (vascular endothelial growth factor/vascular permeability factor receptor) antibody
    • Fms related tyrosine kinase 1 vascular endothelial growth factor/vascular permeability factor receptor antibody
    • Fms-like tyrosine kinase 1 antibody
    • FRT antibody
    • Soluble VEGF receptor 1 14 antibody
    • Soluble VEGFR1 variant 2 antibody
    • Soluble VEGFR1 variant 21 antibody
    • Tyrosine protein kinase FRT antibody
    • Tyrosine protein kinase receptor FLT antibody
    • Tyrosine-protein kinase FRT antibody
    • Tyrosine-protein kinase receptor FLT antibody
    • Vascular endothelial growth factor receptor 1 antibody
    • Vascular endothelial growth factor vascular permeability factor receptor antibody
    • Vascular permeability factor receptor 1 antibody
    • Vascular permeability factor receptor antibody
    • VEGFR 1 antibody
    • VEGFR-1 antibody
    • VEGFR1 antibody
    • VGFR1_HUMAN antibody
    see all

Images

  • ab2350 staining the VEGF Receptor 1 in Mouse lung tissue sections by Immunohistochemistry (IHC-P - formaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 hour at room temperature; antigen retrieval was by heat mediation in citrate buffer (pH 6). Samples were incubated with primary antibody (1/100) for 16 hours at 4°C. An undiluted biotin-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.

    See Abreview

  • ICC/IF image of ab2350 stained A431 (human epidermoid carcinoma cell line) cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2350, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab2350 - immunohistochemistry

    Formalin fixed paraffin embedded human angiosarcoma stained with VEGF Receptor 1, using ABC and AEC chromogen.

References

This product has been referenced in:
See all 32 Publications for this product

Customer reviews and Q&As

1-7 of 7 Abreviews or Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Lung)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate
Permeabilization
No
Specification
Lung
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted May 12 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (lung)
Specification
lung
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH6
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C

Mr. Manoj Kumar Valluru

Verified customer

Submitted Jun 11 2010

Question
Answer

Thank you for your enquiry. This antibody has not been tested on canine tissues. We do not give discounts on products in order to test the antibody, but we do have a rewards program. If you use the antibody on canine tissue, please consider our AbTrial testing program. For more information see www.abcam.com/abtrial

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Question
Answer

Thank you for your e-mail. Amplification is a method to amplify signal on immunohistochemical preparations where there is low abundance of the protein of interest (or the primary antibody binding affinity for the protein is low). The most common amplification method is the ABC method, where this procedure employs biotinylated antibody and a preformed Avidin: Biotinylated enzyme Complex and has been termed the “ABC” technique. Because avidin has such an extraordinarily high affinity for biotin (over one million times higher than antibody for most antigens), the binding of avidin to biotin is essentially irreversible. In addition, avidin has four binding sites for biotin, and most proteins including enzymes can be conjugated with several molecules of biotin. The high sensitivity reported for this technique likely resides in either the form or the number of active enzyme molecules associated with the complex and the rapid, irreversible interaction of the complex with biotinylated antibody. In addition to high sensitivity, ABC kits produce very low background staining. In summary, following incubation of the primary antibody (rabbit), wash in PBS your sections/cells, then add an anti-rabbit secondary antibody which is biotin conjugated, incubate for 2-3 hours, then wash in PBS and add HRP-conjugated avidin (provided in a kit, such as the Vector ABC kit). The avidin will bind to the biotin with high affinity and provide an amplification. When DAB will be added at the last step the numerous HRP proteins will give more signal and you will be able to visualise the localisation of your protein of interest.

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Answer

I would recommend a preliminary experiment using a range of dilutions: 1:200, 1:500, 1:1000, 1:3000 for example and optimise the dilution following the outcome of this experiment.

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Answer

We do not have any data on the whether it recognises the phospho VEGFR1. We would guess that it would detect both phosphorylated and non-phosphorylated since it was raised against a non-phosphorylated peptide. However, we would be very interested to hear if this is the case

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Answer

The synthetic peptide sequence for Flt-1 Ab-1: a.a. 1312-1328 (Cys-AELERKIACCSPPPDYN)

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