Product nameAnti-VEGF Receptor 1 antibody
See all VEGF Receptor 1 primary antibodies
DescriptionRabbit polyclonal to VEGF Receptor 1
Tested applicationsSuitable for: IHC-P, Electron Microscopy, WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human, Pig
Synthetic peptide within Human VEGF Receptor 1 aa 1300 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: P17948
- IHC-P: Mouse lung tissue; human angiosarcoma tissue. ICC/IF: A431 cell line
VEGFR 1 is a cell membrane receptor kinase. It has a high affinity receptor for vascular endothelial growth factor and is putatively involved in the growth of endothelial cells and angiogenesis.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.1% Sodium azide
Constituents: 0.0268% PBS, 1% BSA
Concentration information loading...
PurityProtein A purified
Primary antibody notesVEGFR 1 is a cell membrane receptor kinase. It has a high affinity receptor for vascular endothelial growth factor and is putatively involved in the growth of endothelial cells and angiogenesis.
Our Abpromise guarantee covers the use of ab2350 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|Electron Microscopy||1/100. PubMed: 18647955|
|WB||Use at an assay dependent concentration. PubMed: 21912702|
|ICC/IF||Use a concentration of 5 µg/ml.|
FunctionReceptor for VEGF, VEGFB and PGF. Has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. Isoform SFlt1 may have an inhibitory role in angiogenesis.
Tissue specificityMostly in normal lung, but also in placenta, liver, kidney, heart and brain tissues. Specifically expressed in most of the vascular endothelial cells, and also expressed in peripheral blood monocytes. Isoform sFlt1 is strongly expressed in placenta.
Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
Contains 7 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain.
Cellular localizationSecreted and Cell membrane.
- Information by UniProt
- EC 184.108.40.206 antibody
- FLT 1 antibody
- FLT antibody
ab2350 staining the VEGF Receptor 1 in Mouse lung tissue sections by Immunohistochemistry (IHC-P - formaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 hour at room temperature; antigen retrieval was by heat mediation in citrate buffer (pH 6). Samples were incubated with primary antibody (1/100) for 16 hours at 4°C. An undiluted biotin-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.
ICC/IF image of ab2350 stained A431 (human epidermoid carcinoma cell line) cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2350, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab2350 - immunohistochemistry
Formalin fixed paraffin embedded human angiosarcoma stained with VEGF Receptor 1, using ABC and AEC chromogen.
This product has been referenced in:
- Chua JS et al. A glycan-based approach to therapeutic angiogenesis. PLoS One 12:e0182301 (2017). Read more (PubMed: 28763512) »
- Krzywinska E et al. Loss of HIF-1a in natural killer cells inhibits tumour growth by stimulating non-productive angiogenesis. Nat Commun 8:1597 (2017). Read more (PubMed: 29150606) »