Anti-VEGF Receptor 1 antibody [Y103] - Low endotoxin, Azide free (ab184784)

Blocking buffer and concentration: 5% NFDM/TBST

Diluting buffer and concentration: 5% NFDM/TBST

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human abdominal aortic aneurysm (AAA) wall tissue sections labeling VEGF Receptor 1 with ab32152 at 1/100 dilution.

Resected aortic tissues were immersed in 10% neutral buffered formalin for at least 24 h for immunohistochemical staining. Tissue sample was embedded in paraffin; 4 µm sections were cut and mounted onto MAS-coated slides. The sections were deparaffinized, dehydrated, and boiled in a pressure cooker in 0.01 M citric acid buffer (pH 6.0) for 20 min. The sections were washed with phosphate-buffered saline and incubated with 3% H₂O₂ in absolute methanol for 5 min to inhibit any endogenous peroxidase activity. Sections were preincubated with 3% normal goat serum for 20 min to minimize nonspecific binding to VEGF Receptor 1, and incubated with ab32152 at 4°C overnight in a moist chamber. The section was washed with phosphate-buffered saline and then incubated with the appropriate secondary antibody for 30 min at room temperature. Staining was visualized with Vector DAB, and tissue section was then counterstained with hematoxylin.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32152).

Immunohistochemistry (PFA perfusion fixed frozen sections) analysis of mouse brain tissue section (15 days old wild-type mouse embryonic brain, 16 micron) labeling VEGF Receptor 1 with ab32152 at 1/300 dilution.

Tissue was fixed with formaldehyde and permeabilized with Triton X-100. Heat mediated antigen retrieval was performed using 10mM citrate buffer, pH 6. A polyclonal donkey anti-Rabbit IgG (H+L) (Alexa Fluor^® 488) secondary antibody was used at 1/500 dilution. 

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32152).