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The antibody (ab10972) you have suggested is the one I originally used and it did not work. I need you to suggest another one which is not raised in rabbit but is also not the one I have already bought!
Please get back to me when you get the other reply, or with any other suggestions in the mean time
Asked on Dec 03 2012
Thank you for your reply. I have now returned to the office an hope I can help further with your enquiry.
I can now confirm that of the antibodies we have directed against VEGF Receptor 2 (that are not raised in rabbit), most likely to react with the sheep protein are the mouse monoclonals ab10972, ab10973 and ab82266. This is based on the immunogen used to raise these antibodies. They have all be raised against the extracellular domain of the VEGF Receptor 2, however, this region covers the residues 1-745 and as I only have the region between 230-400 of the sheep protein to compare I cannot be at all certain that they will work, as you have observed with ab10972. We have ourselves not tested any of these antibodies with samples from sheep and therefore cannot guarantee their performance.
If you would like, I can offer a testing discount with ab10973 or ab82266. More information on this offer can be found from the following link:
However, I would suggest the rabbit antibodies I suggested, ab39521, ab62697, ab63613 and ab131510 (especially ab131510 as it has been used in ICC/IF as well as staining of paraffin embedded sections) would be more likely to work with your samples.
Again, if you would like to participate in the Abtrial with any of these antibodies please do let me know.
I hope this information has been of some help. If I can be of any further assistance, please do not hesitate to let me know.
Answered on Dec 03 2012