Key features and details
- Mouse monoclonal [#4 (20I6)] to VEGF Receptor 2
- Suitable for: WB, ICC/IF, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-VEGF Receptor 2 antibody [#4 (20I6)]
See all VEGF Receptor 2 primary antibodies
DescriptionMouse monoclonal [#4 (20I6)] to VEGF Receptor 2
Tested applicationsSuitable for: WB, ICC/IF, Flow Cytmore details
Species reactivityReacts with: Human
Recombinant full length protein corresponding to Human VEGF Receptor 2 aa 20-678.
ASVGLPSVSLDLPRLSIQKDILTIKANTTLQITCRGQRDLDWLWPNNQSG SEQRVEVTECSDGLFCKTLTIPKVIGNDTGAYKCFYRETDLASVIYVYVQ DYRSPFIASVSDQHGVVYITENKNKTVVIPCLGSISNLNVSLCARYPEKR FVPDGNRISWDSKKGFTIPSYMISYAGMVFCEAKINDESYQSIMYIVVVV GYRIYDVVLSPSHGIELSVGEKLVLNCTARTELNVGIDFNWEYPSSKHQH KKLVNRDLKTQSGSEMKKFLSTLTIDGVTRSDQGLYTCAASSGLMTKKNS TFVRVHEKPFVAFGSGMESLVEATVGERVRIPAKYLGYPPPEIKWYKNGI PLESNHTIKAGHVLTIMEVSERDTGNYTVILTNPISKEKQSHVVSLVVYV PPQIGEKSLISPVDSYQYGTTQTLTCTVYAIPPPHHIHWYWQLEEECANE PSQAVSVTNPYPCEEWRSVEDFQGGNKIEVNKNQFALIEGKNKTVSTLVI QAANVSALYKCEAVNKVGRGERVISFHVTRGPEITLQPDMQPTEQESVSL WCTADRSTFENLTWYKLGPQPLPIHVGELPTPVCKNLDTLWKLNATMFSN STNDILIMELKNASLQDQGDYVCLAQDRKTKKRHCVVRQLTVLGRETILD HCAEAVGMP
Database link: P35968-2
- WB: Recombinant human VEGF Receptor 2 protein. ICC/IF: HUVEC cells. Flow Cyt: HDLEC cells.
FormLyophilized:Centrifuge vial prior to opening. Please reconstitute this product to 0.1-1.0 mg/mL in sterile water.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). The lyophilized protein is stable for a few weeks at room temperature. Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferConstituent: PBS
Concentration information loading...
PurityProtein G purified
Clone number#4 (20I6)
Our Abpromise guarantee covers the use of ab39378 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 2 - 5 µg/ml. Predicted molecular weight: 110 kDa.|
|ICC/IF||Use a concentration of 2 - 10 µg/ml.|
|Flow Cyt||Use a concentration of 2 - 5 µg/ml.|
FunctionReceptor for VEGF or VEGFC. Has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
Involvement in diseaseDefects in KDR are associated with susceptibility to hemangioma capillary infantile (HCI) [MIM:602089]. HCI are benign, highly proliferative lesions involving aberrant localized growth of capillary endothelium. They are the most common tumor of infancy, occurring in up to 10% of all births. Hemangiomas tend to appear shortly after birth and show rapid neonatal growth for up to 12 months characterized by endothelial hypercellularity and increased numbers of mast cells. This phase is followed by slow involution at a rate of about 10% per year and replacement by fibrofatty stroma.
Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
Contains 7 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain.
modificationsPhosphorylated. Dephosphorylated by PTPRB. Dephosphorylated by PTPRJ at Tyr-951, Tyr-996, Tyr-1054, Tyr-1059, Tyr-1175 and Tyr-1214.
- Information by UniProt
- CD309 antibody
- CD309 antigen antibody
- EC 126.96.36.199 antibody
All lanes : Anti-VEGF Receptor 2 antibody [#4 (20I6)] (ab39378) at 5 µg/ml
Lane 1 : Recombinant human VEGF Receptor 2 protein
Lane 2 : Recombinant human VEGF Receptor 2 (aa 20-678) protein
Lane 3 : Recombinant human VEGF Receptor 2 (aa 20-678) protein-Fc
Predicted band size: 110 kDa
10% SDS-PAGE gel under reducing conditions.
Flow cytometric analysis of HDLEC (primary human dermal lymphatic endothelial cells) labeling VEGF Receptor 2 using ab39378 at 5 μg/ml (red) compared to a negative control (unshaded).
HUVEC (human umbilical vein endothelial cell line) cells stained for VEGF Receptor 2 (green) using ab39378 at 7.5 μg/ml in ICC/IF. The nuclear counter stain is DAPI (blue).
A Goat anti-Mouse Alexa Fluor®488 conjugate was used as secondary antibody at 1/600 dilution.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab39378 has not yet been referenced specifically in any publications.