Overview

  • Product name
    Anti-VEGF Receptor 2 antibody
    See all VEGF Receptor 2 primary antibodies
  • Description
    Goat polyclonal to VEGF Receptor 2
  • Host species
    Goat
  • Specificity
    Based on immunoblotting and ELISA, this antibody shows approximately 2% cross-reactivity with recombinant mouse VEGF Receptor 1, and no cross-reactivity with recombinant human VEGF Receptor 1 and VEGF Receptor 3.
  • Tested applications
    Suitable for: ICC/IF, Indirect ELISA, IHC-P, Flow Cyt, ICC, WB, Neutralising, Blockingmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Dog
    Does not react with: Human
  • Immunogen

    Recombinant fragment from the extracellular domain (Mouse) expressed in mouse NSO cells.

  • General notes
    Do not store in frost-free freezer. Endotoxin level is < 10 ng/mg antibody as determined by the LAL (Limulus amebocyte lysate) method.

Applications

Our Abpromise guarantee covers the use of ab10972 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 - 15 µg/ml. (and tissues).
Indirect ELISA Use a concentration of 0.5 - 1 µg/ml.
IHC-P Use at an assay dependent concentration. PubMed: 20882677
Flow Cyt Use a concentration of 3 - 10 µg/ml.

ab37373 - Goat polyclonal IgG, is suitable for use as an isotype control with this antibody.

ICC Use at an assay dependent concentration. See Abreview.
WB Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 152 kDa.
Neutralising Use at an assay dependent concentration.
Blocking Use at an assay dependent concentration. PubMed: 20884890

Target

  • Function
    Receptor for VEGF or VEGFC. Has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
  • Involvement in disease
    Defects in KDR are associated with susceptibility to hemangioma capillary infantile (HCI) [MIM:602089]. HCI are benign, highly proliferative lesions involving aberrant localized growth of capillary endothelium. They are the most common tumor of infancy, occurring in up to 10% of all births. Hemangiomas tend to appear shortly after birth and show rapid neonatal growth for up to 12 months characterized by endothelial hypercellularity and increased numbers of mast cells. This phase is followed by slow involution at a rate of about 10% per year and replacement by fibrofatty stroma.
  • Sequence similarities
    Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
    Contains 7 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Phosphorylated. Dephosphorylated by PTPRB. Dephosphorylated by PTPRJ at Tyr-951, Tyr-996, Tyr-1054, Tyr-1059, Tyr-1175 and Tyr-1214.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD309 antibody
    • CD309 antigen antibody
    • EC 2.7.10.1 antibody
    • Fetal liver kinase 1 antibody
    • FLK-1 antibody
    • FLK1 antibody
    • FLK1, mouse, homolog of antibody
    • Kdr antibody
    • Kinase insert domain receptor (a type III receptor tyrosine kinase) antibody
    • Kinase insert domain receptor antibody
    • KRD1 antibody
    • Ly73 antibody
    • Protein tyrosine kinase receptor FLK1 antibody
    • Protein-tyrosine kinase receptor flk-1 antibody
    • soluble VEGFR2 antibody
    • Tyrosine kinase growth factor receptor antibody
    • Vascular endothelial growth factor receptor 2 antibody
    • VEGFR 2 antibody
    • VEGFR antibody
    • VEGFR-2 antibody
    • VEGFR2 antibody
    • VGFR2_HUMAN antibody
    see all

Images

  • Ab10972 positively staining VEGF receptor 2 in paraformaldehyde fixed mouse retinal pigment epithelium (10 µg/ml). Used in conjunction with donkey anti goat IgG (Alexa ® 488) at 1/100.

    This image is courtesy of an Abreview submitted on 12th October 2005. We do not have any further information relating to this image.

References

This product has been referenced in:
  • Shen M  et al. Xanthatin inhibits corneal neovascularization by inhibiting the VEGFR2-mediated STAT3/PI3K/Akt signaling pathway. Int J Mol Med 42:769-778 (2018). Read more (PubMed: 29717775) »
  • Wang TC  et al. Characterization of highly proliferative secondary tumor clusters along host blood vessels in malignant glioma. Mol Med Rep 12:6435-44 (2015). IHC-Fr ; Rat . Read more (PubMed: 26299849) »
See all 8 Publications for this product

Customer reviews and Q&As

1-10 of 22 Abreviews or Q&A

Answer

Thank you for contacting us. I ran a search in our catalogue according to the parameters you requested and came up with a list of about 37 antibodies (attached).  I don't think that looking for MHC Class antibodies will help since these are probably limited by the type of mice you're studying. What may be best from the list attached would be the anti-VEGFR2 antibody ab10972.  https://www.abcam.com/VEGF-Receptor-2-antibody-ab10972.html From a quick literature search online, it looks like this protein has been investigated in NSCLC studies, but I am definitely not an expert in NSCLC so I'd recommend that you look into this further and check out the other targets in the attached list, as well. http://www.ncbi.nlm.nih.gov/pubmed/20549821 http://www.asco.org/ascov2/Meetings/Abstracts?&vmview=abst_detail_view&confID=65&abstractID=30831 I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.  

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Answer

Thank you for your reply. I have now returned to the office an hope I can help further with your enquiry.

I can now confirm that of the antibodies we have directed against VEGF Receptor 2 (that are not raised in rabbit), most likely to react with the sheep protein are the mouse monoclonals ab10972, ab10973 and ab82266. This is based on the immunogen used to raise these antibodies. They have all be raised against the extracellular domain of the VEGF Receptor 2, however, this region covers the residues 1-745 and as I only have the region between 230-400 of the sheep protein to compare I cannot be at all certain that they will work, as you have observed with ab10972. We have ourselves not tested any of these antibodies with samples from sheep and therefore cannot guarantee their performance.

If you would like, I can offer a testing discount with ab10973 or ab82266. More information on this offer can be found from the following link:

www.abcam.com/abtrial

However, I would suggest the rabbit antibodies I suggested, ab39521, ab62697, ab63613 and ab131510 (especially ab131510 as it has been used in ICC/IF as well as staining of paraffin embedded sections) would be more likely to work with your samples.

Again, if you would like to participate in the Abtrial with any of these antibodies please do let me know.

I hope this information has been of some help. If I can be of any further assistance, please do not hesitate to let me know.

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Answer


Reviewing the correspondence on this enquiry so far, I notice xxxxx has selected some antibodies not raised in rabbit and has been in contact with the originators to obtain further information regarding the immunogen sequence so we can work out whether they would detect the two fragments of sequence previously discussed (from SwissProt (Q8MI25 and Q8MI23) align to ˜230-400 and 1020-1200 of human and mouse VEGF Receptor 2 respectively).

I apologise for the delay so far. We are waiting for one more reply. However, the most promising response we have had is regarding ab10972 Anti-VEGF Receptor 2 antibody. The immunogen used to raise this product was purified recombinant mouse VEGF R2 extracellular domain expressed in NSO cells. The human extracellular domain (soluble) corresponds to residues 1-745. We do not know for sure whether these are the exact mouse protein residues in the immunising sequence but it is likely to cover residues 230-400 that has been specified in previous emails.

Please note that regrettably we are not able to guarantee this antibody for Sheep as it has not been tested. The applications it has been tested and guaranteed for are ICC/IF, Indirect ELISA, IHC-P, Flow Cyt, ICC, WB, Neutralising, Blocking.

If you would like to test this antibody in sheep or an untested application, please contact me again by replying to this message prior to the purchase as you may be eligible for our AbTrial testing discount program.
Otherwise, we like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates.


To find out more about our Abreview system, please see the following link:

https://www.abcam.com/abreviews

I am sorry we do not have a suitably tested antibody to suggest on this occasion. However, I hope this information is helpful. Please do not hesitate to contact me if you have any further questions or concerns.

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Question
Answer

Thank you for contacting us this morning in regards to the anti-VEGF Receptor 2 antibody ab10972 and ab9530. I am sorry for the misunderstanding there seems to have been in this case.

As discussed over the phone, I am currently attempting to compare the homology of the sheep protein with that of the immunogen used to raise the antibodies ab10972 and ab9530. However, I am currently having some difficulty in finding a good protein sequence for the sheep protein. I have found the SwissProt references http://www.uniprot.org/uniprot/Q8MI25 and http://www.uniprot.org/uniprot/Q8MI23 but I could not find a complete sequence.

I was therefore wondering if you could share the sequence you have for this protein? I can then check which antibodies in our catalogue are most likely to cross react.

I look forward to receiving your reply.

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Answer

Thank you for contacting us.

The datasheet does not state that the antibody does not work with human samples so I am sorry this actually voids the guarantee (https://www.abcam.com/index.html?pageconfig=abpromise) for replacement.

I would suggest you to try mouse, rat or dog cells as positive control for checking the quality of protocol and antibodies as well. In case the antibody does not give results with mouse rat or dog cells, let us know we will be happy to help further.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Answer

Thank you for contacting us.

I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

I am attaching our questionnaire so that we can gather further information regarding the samples tested and the protocol used. Once we receive the completed questionnaire, we will look at the protocol and see if there are any suggestions we can make that may improve the results. This information will also allow us to investigate this case internally and initiate additional testing where necessary. If the product was purchased in the last six months and is being used according to our Abpromise, we would be happy to replace or refund the antibody.

I look forward to receiving your reply.

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Answer

Thank you for contacting us.

We recommend ab96917, a rabbit anti-goat IgG secondary antibody that is pre-adsorbed to remove reactivity with rat IgG, among other species, and is conjugated to DyLight® 594.

Click here (or use the following: https://www.abcam.com/index.html?datasheet=96917).

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Answer

Thank you for contacting us. I apologise for the delay and appreciate your patient and comprehension.

The immunogen for ab10972 and ab10975 consists of recombinant VEGF2-R extracellular domain. For ab10972 the immunogen was expressed in mouse NSO cells and ab10975 the immunogen was expressed in the baculovirus system (insect cells).

From a quick literature search it appears that the phosphorylation sites are within the cytoplasmic region of VEGF2-R. As both of these antibodies are raised against the extracellular region of the receptor, I would expect them to recognise any form of the receptor that contains the extracellular domain. I would not expect them to differentiate between phosphorylated and non-phosphorylated receptor, though we have not tested this.

In regards to PDGF receptor beta, we have antibodies recognising specific phosphorilation sites, but similarly to the VEGF2-R unless it is stated on the datasheet, we have not tested further specificity.

https://www.abcam.com/index.html?pageconfig=searchresults&search=PDGF&pt=1&sk=react&sv=21&sn=Human&l=3&fViewMore=1

I hope this helps and if we can assist further, please do not hesitate to contact us.

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Answer

I am very pleased to hear you would like to accept our offer and testab10972 in IHC whole mount. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for IHC whole mount and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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Application
Western blot
Sample
Human Cell lysate - whole cell (A549)
Loading amount
20 µg
Specification
A549
Treatment
TRAIL
Gel Running Conditions
Reduced Denaturing (12.5% SDS-PAGE)
Blocking step
(agent) for 2 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 20°C

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1-10 of 22 Abreviews or Q&A

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