Anti-VEGF Receptor 2 antibody (ab2349)

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry the products did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number ######.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

Thank you for taking the time to complete our questionnaire.

The details you have kindly provided will provide us with vital information for our monitoring of product quality.

I appreciate the time the customer have spent in the laboratory and understand their concerns. It is regrettable the results have not been successful. Reviewing the details, I am sorry there very few tips to provide on this occasion to help improve the results from either of these antibodies. I can suggest it is possible you may have regrettably received a bad vial on this occasion.

However, I can recommend it would be important to consider the following:

ab10728 WB
1. Has the protein transfer to the membrane and quality of the sample been assessed using a loading control?
2. Is the current vial of secondary antibody working well with other primary antibodies?
3. I can suggest to try increasing the antibody concentration, for example 0.5 or even 1 ug/ml. The dilution provided on the datasheet is a guideline only. Increasing the concentration may help increase the signal.

ab2349
1. Antibodies are usually used at quite a high concentration in flow cytometry, often at a dilution between 1:10 to 1:50. Has this been tried? It may increase the signal obtained.
2. Is the current vial of secondary antibody working well with other primary antibodies?

Alternatively, or if these tips do not work, I am pleased to offer you a free of charge replacement or credit note for each antibody in compensation.

Thank you for your cooperation. I look forward to hearing from you with details of how you would like to proceed.

Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

I would like to reassure you that our antibody are tested and covered by our 6 month guarantee for the tested applications and species listed on the individual datasheets. In the event that a product is not functioning in the tested applications and species cited on the product data sheet (and the problem has been reported within 6 months of purchase), we will be pleased to provide a credit note, free of charge replacement or refund.

I appreciate your concerns and it is regrettable the results have not been successful. Before deciding how to proceed in this case, I would like to investigate this particular case further for you, and also obtain more information for our quality monitoring records. In order to proceed with this, I have enclosed a technical questionnaire below. I would appreciate if you could complete this. It will help you put the information we require together very easily.

Thank you for your time and cooperation. We look forward to receiving the completed questionnaire.

WESTERN BLOT:


Order Details
Antibody code:

Problem
Choose: Non-specific band Multiple bands No signal or weak signal High background

Lot number

Purchase order number
or preferably Abcam order number:



General Information
Antibody storage conditions (temperature/reconstitution etc)


Description of the problem (high background, wrong band size, more bands, no band etc.)


Sample (Species/Cell extract/Nuclear extract/Purified protein/Recombinant protein etc.)


Sample preparation (Buffer/Protease inhibitors/Heating sample etc.)


Amount of protein loaded


Electrophoresis/Gel conditions (Reducing or Non-reducing gel, % of the gel etc.)


Transfer and blocking conditions (Buffer/time period, Blocking agent etc.)


Primary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step)


Secondary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step)


Detection method (ECL, ECLPlus etc.)


Positive and negative controls used (please specify)



Optimization attempts (problem solving)
How many times have you tried the Western?



Have you run a "No Primary" control?
Yes No

Do you obtain the same results every time?
Yes No
e.g. are the background bands always in the same place?


What steps have you altered?


Additional Notes:


Image:
We would appreciate if you are able to provide an image (including molecular weight markers) which would help us to asess the results.




FLOW CYTOMETRY

Order Details
Antibody code:


Problem:
Choose: Non-specific staining No signal or weak signal High background


Lot number

Purchase order number
or preferably Abcam order number


General Information
Antibody storage conditions (temperature/reconstitution etc)


Description of the problem (high background, low signal, no signal etc.)


Sample (Species/Cell type/Cell line etc.)


Sample preparation for single cell preparation (Buffer etc.)


Number of cells used


Permeabilization, fixation step


Blocking conditions (Buffer/time period, Blocking agent etc.)


Primary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step)


Secondary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step)


Detection method


Positive and negative controls used (please specify)




Optimization attempts (problem solving)
How many times have you tried the FACS?



Have you run a "No Primary" control?


Yes No
Do you obtain the same results every time?
Yes No

What steps have you altered?


Additional Notes:


Data:
We would appreciate if you are able to provide any data/histograms which will help us to assess the results

Thank you for your message and for providing this further information.

I appreciate the time your customerhas spent on these experiments and would be pleased to arrange in compensationa credit note ora replacement with either a different lot of ab2349 or an alternative anti-VEGF receptor 2 such as ab39256, ab39638, ab131441, ab45010 orab131510.

I look forward to hearing from you with details of how you and your customerwould like to proceed.

Thank you for contacting Abcam regarding ab2349.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

Thank you for your reply. xxxxis currently away from the office but I hope I can provide you with the information you have requested.

The Anti-VEGF Receptor 2 antibody (ab2349) has only so far been validated inhouse with human tissue in IHC. The information regarding its reactivity with rat samples has come from customer feedback as well as published articles:

Mafuvadze B et al. Apigenin prevents development of medroxyprogesterone acetate-accelerated 7,12-dimethylbenz(a)anthracene-induced mammary tumors in Sprague-Dawley rats. Cancer Prev Res (Phila) 4:1316-24 (2011). PubMed: 21505181

Benakanakere I et al. Synthetic Progestins Differentially Promote or Prevent 7,12-Dimethylbenz(a)anthracene-Induced Mammary Tumors in Sprague-Dawley Rats. Cancer Prev Res (Phila Pa) 3:1157-67 (2010). PubMed: 20699413

Seabrook TJ et al. Angiogenesis is present in experimental autoimmune encephalomyelitis and pro-angiogenic factors are increased in multiple sclerosis lesions. J Neuroinflammation 7:95 (2010). PubMed: 21176212

Koleganova N et al. A calcimimetic (R-568), but not calcitriol, prevents vascular remodeling in uremia. Kidney Int 75:60-71 (2009). PubMed: 19092814

Cheluvappa R et al. The effect of old age on liver oxygenation and the hepatic expression of VEGF and VEGFR2. Exp Gerontol 42:1012-9 (2007). PubMed: 17658234

The IHC-P protocol followed with this antibody inhouse was performed using HIER withCitrate Buffer pH 6.0 and the antibody was detected using an HRP polymer using DAB and hematoxylin used as a counter stain.

If you require any further information please do let us know.

Until then, I wish you all the best with your research.