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    vegf-receptor-2-antibody-ab2349.pdf

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Cardiovascular Angiogenesis Growth Factors VEGF VEGF Receptors
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Anti-VEGF Receptor 2 antibody (ab2349)

  • Datasheet
  • SDS
Reviews (16)Q&A (18)References (101)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGF Receptor 2 antibody (ab2349)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGF Receptor 2 antibody (ab2349)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGF Receptor 2 antibody (ab2349)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGF Receptor 2 antibody (ab2349)

Key features and details

  • Rabbit polyclonal to VEGF Receptor 2
  • Suitable for: IHC-P
  • Reacts with: Human
  • Isotype: IgG

You may also be interested in

Protein
Product image
Recombinant human VEGF Receptor 2 protein (Active) (ab106310)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-VEGF Receptor 2 antibody
    See all VEGF Receptor 2 primary antibodies
  • Description

    Rabbit polyclonal to VEGF Receptor 2
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide:

    KMVDAAVHADSGTTLQLTSC

    , corresponding to C terminal amino acids 1326-1345 of Mouse VEGF Receptor 2.
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • IHC-P: Human normal breast and angiosarcoma tissues; Mouse skin tissue.
  • General notes

    Isoform 1 localization: Cell membrane; Single-pass type I membrane protein. Cytoplasm. Nucleus. Cytoplasmic vesicle. Early endosome.

    Note: Detected on caveolae-enriched lipid rafts at the cell surface. Is recycled from the plasma membrane to endosomes and back again. Phosphorylation triggered by VEGFA binding promotes internalization and subsequent degradation. VEGFA binding triggers internalization and translocation to the nucleus.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituent: 1% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Angiogenesis
    • Growth Factors
    • VEGF
    • VEGF Receptors
    • Microbiology
    • Interspecies Interaction
    • Host Virus Interaction
    • Signal Transduction
    • Protein Phosphorylation
    • Tyrosine Kinases
    • Receptor Tyrosine Kinases
    • Signal Transduction
    • Growth Factors/Hormones
    • VEGF
    • Stem Cells
    • Hematopoietic Progenitors
    • Surface Molecules
    • Cancer
    • Growth factors
    • VEGF
    • Cancer
    • Invasion/microenvironment
    • Angiogenesis
    • Angiogenic growth factors
    • Cancer
    • Oncoproteins/suppressors
    • Oncoproteins
    • Growth factor receptors
    • Stem Cells
    • Endothelial Progenitors
    • Endothelial Markers
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Cardiovascular
    • Cardiovascular Markers
    • Cell Markers
    • Endothelial Cells
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia
    • Metabolism
    • Types of disease
    • Cancer
    • Cardiovascular
    • Angiogenesis
    • Endothelial Cell Markers

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant human VEGF Receptor 2 protein (Active) (ab106310)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab2349 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-P
Human
All applications
Mouse
Application Abreviews Notes
IHC-P (7)
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Receptor for VEGF or VEGFC. Has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
  • Involvement in disease

    Defects in KDR are associated with susceptibility to hemangioma capillary infantile (HCI) [MIM:602089]. HCI are benign, highly proliferative lesions involving aberrant localized growth of capillary endothelium. They are the most common tumor of infancy, occurring in up to 10% of all births. Hemangiomas tend to appear shortly after birth and show rapid neonatal growth for up to 12 months characterized by endothelial hypercellularity and increased numbers of mast cells. This phase is followed by slow involution at a rate of about 10% per year and replacement by fibrofatty stroma.
  • Sequence similarities

    Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
    Contains 7 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications

    Phosphorylated. Dephosphorylated by PTPRB. Dephosphorylated by PTPRJ at Tyr-951, Tyr-996, Tyr-1054, Tyr-1059, Tyr-1175 and Tyr-1214.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession P35968 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 3791 Human
    • Entrez Gene: 16542 Mouse
    • Omim: 191306 Human
    • SwissProt: P35968 Human
    • SwissProt: P35918 Mouse
    • Unigene: 479756 Human
    • Unigene: 285 Mouse
    • Alternative names

      • CD309 antibody
      • CD309 antigen antibody
      • EC 2.7.10.1 antibody
      • Fetal liver kinase 1 antibody
      • FLK-1 antibody
      • FLK1 antibody
      • FLK1, mouse, homolog of antibody
      • Kdr antibody
      • Kinase insert domain receptor (a type III receptor tyrosine kinase) antibody
      • Kinase insert domain receptor antibody
      • KRD1 antibody
      • Ly73 antibody
      • Protein tyrosine kinase receptor FLK1 antibody
      • Protein-tyrosine kinase receptor flk-1 antibody
      • soluble VEGFR2 antibody
      • Tyrosine kinase growth factor receptor antibody
      • Vascular endothelial growth factor receptor 2 antibody
      • VEGFR 2 antibody
      • VEGFR antibody
      • VEGFR-2 antibody
      • VEGFR2 antibody
      • VGFR2_HUMAN antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGF Receptor 2 antibody (ab2349)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGF Receptor 2 antibody (ab2349)

      Formalin fixed, paraffin-embedded human angiosarcoma tissue stained for VEGF Receptor 2 using ab2349 at 1/50 dilution in immunohistochemical analysis.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGF Receptor 2 antibody (ab2349)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGF Receptor 2 antibody (ab2349)This image is courtesy of an Abreview submitted by Manoj Kumar Valluru
      ab2349 staining the VEGF Receptor 2 in Mouse skin tissue sections by Immunohistochemistry (IHC-P - formaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 hour at room temperature; antigen retrieval was by heat mediation in citrate buffer (pH 6). Samples were incubated with primary antibody (1/100 in PBS + 2% blocking serum) for 16 hours at 4°C. A biotin-conjugated Goat anti-rabbit IgG polyclonal (1/250) was used as the secondary antibody.

      See Abreview

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGF Receptor 2 antibody (ab2349)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGF Receptor 2 antibody (ab2349)Image from Arias-Pulido, Hugo et al., BMC Cancer 12 (2012): 298. PMC. Web. 25 Jan. 2017. doi: 10.1186/1471-2407-12-298. Fig 1I. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/2.0/.

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human normal breast tissue labeling VEGF Receptor 2 with ab2349 at 1/200 dilution. Sections were lightly counterstained with hematoxylin.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGF Receptor 2 antibody (ab2349)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGF Receptor 2 antibody (ab2349)Image from Arias-Pulido, Hugo et al., BMC Cancer 12 (2012): 298. PMC. Web. 25 Jan. 2017. doi: 10.1186/1471-2407-12-298. Fig 1H. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/2.0

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human normal breast tissue labeling VEGF Receptor 2 with ab2349 at 1/200 dilution. Sections were lightly counterstained with hematoxylin.

    Protocols

    • Flow cytometry protocols
    • Immunoprecipitation protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (101)

    Publishing research using ab2349? Please let us know so that we can cite the reference in this datasheet.

    ab2349 has been referenced in 101 publications.

    • Sun Y  et al. microRNA-124-3p inhibits tumourigenesis by targeting mitogen-activated protein kinase 4 in papillary thyroid carcinoma. Cell Biochem Funct N/A:N/A (2020). PubMed: 32495394
    • Daum R  et al. Fibronectin Adsorption on Electrospun Synthetic Vascular Grafts Attracts Endothelial Progenitor Cells and Promotes Endothelialization in Dynamic In Vitro Culture. Cells 9:N/A (2020). PubMed: 32210018
    • Zhang D  et al. Dexamethasone and lenvatinib inhibit migration and invasion of non-small cell lung cancer by regulating EKR/AKT and VEGF signal pathways. Exp Ther Med 19:762-770 (2020). PubMed: 31853327
    • Wang Z  et al. Sevoflurane promotes the proliferation of HUVECs by activating VEGF signaling. Exp Ther Med 19:1336-1342 (2020). PubMed: 32010307
    • Zhou H  et al. The proangiogenic effects of extracellular vesicles secreted by dental pulp stem cells derived from periodontally compromised teeth. Stem Cell Res Ther 11:110 (2020). PubMed: 32143712
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-10 of 34 Abreviews or Q&A

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-VEGF Receptor 2 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Rat Tissue sections (Kidney)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH 6.0
    Permeabilization
    Yes - 0.05% Tween 20
    Specification
    Kidney
    Blocking step
    Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 24 2020

    Immunocytochemistry/ Immunofluorescence abreview for Anti-VEGF Receptor 2 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Rat Cell (Brain cell culture)
    Permeabilization
    Yes - 0.05% Tween 20
    Specification
    Brain cell culture
    Blocking step
    Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 21 2020

    Immunocytochemistry/ Immunofluorescence abreview for Anti-VEGF Receptor 2 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Kidney H9 Organoids)
    Permeabilization
    Yes - 0.05% Tween 20
    Specification
    Kidney H9 Organoids
    Blocking step
    Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 11 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-VEGF Receptor 2 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (Cerebellum)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: ab64236 100x Citrate Buffer pH 6.0
    Permeabilization
    Yes - 0.05% Tween 20
    Specification
    Cerebellum
    Blocking step
    sea block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Dec 25 2019

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-VEGF Receptor 2 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Breast)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH 6.0
    Permeabilization
    Yes - 0.05% Tween 20
    Specification
    Breast
    Blocking step
    sea block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Dec 25 2019

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-VEGF Receptor 2 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (First trimester human placenta)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Sodium Citrate
    Specification
    First trimester human placenta
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Mar 29 2017

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-VEGF Receptor 2 antibody - Hemangioblast Marker

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Dog Tissue sections (Granulation tissue)
    Antigen retrieval step
    Heat mediated
    Permeabilization
    No
    Specification
    Granulation tissue
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 30% · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More

    Francisco Fernandez Flores

    Verified customer

    Submitted Dec 01 2015

    Immunocytochemistry/ Immunofluorescence abreview for Anti-VEGF Receptor 2 antibody - Hemangioblast Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (iCell® Cardiomyocytes)
    Permeabilization
    No
    Specification
    iCell® Cardiomyocytes
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Acetone
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 25 2015

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-VEGF Receptor 2 antibody - Hemangioblast Marker

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
    Antigen retrieval step
    None
    Sample
    Rat Tissue sections (skin)
    Specification
    skin
    Permeabilization
    No
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Jun 10 2014

    Question

    Dear Techserve, kindly receive customers reply below:

    Thank You for Your reply! Please look below for the answers.

    1. Has the protein transfer to the membrane and quality of the sample been assessed using a loading control?

    We always use a loading control for western blot. The pdf document that I had sent to you earlier indicates beta actin as a loading control clearly.

    2. Is the current vial of secondary antibody working well with other primary antibodies ?

    Yes, it does. We are running several western blottings every day, so our secondary antibodies are almost daily tested by several colleagues.

    The host species for the abcam c-MET primary antibody is goat. For beta actin primary antibody , the host species is goat as well. The secondary antibody used was the same for both these primary antibodies (donkey anti-goat). The beta actin bands are very clear, whereas there were no bands for c-MET. This clearly indicates that the current secondary antibody vial works.

    Similarly, the rabbit secondary antibody used for flow cytometry has been in continuous use in our lab and the current vial works.

    3. Increasing the antibody concentrations

    Regarding the western blotting: if the signal is very mild or weak, then we can enhance the signal using a higher primary antibody concentration, but if we have no signal (even with the femto chemiluminiscence detection), then I personally have doubt that increasing the antibody concentration will help. With respect to flow cytometry, increasing the concentration of primary antibody, we have to increase the corresponding IgG control, which in turn may result in proportional higher signal. Nevertheless, I will take your suggestion and use a higher concentration of the antibodies.

    In the meanwhile, could we kindly proceed with this decision of replacement of the antibodies.

    Await your reply thankfully!

    Read More

    Abcam community

    Verified customer

    Asked on Nov 27 2012

    Answer

    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

    I am sorry the products did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number ######.

    To check the status of the order please contact our Customer Service team and reference this number.

    Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

    I wish you the best of luck with your research.

    Read More

    Abcam Scientific Support

    Answered on Nov 27 2012

    1-10 of 34 Abreviews or Q&A

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