Overview

  • Product name
    Anti-VEGF Receptor 2 antibody
    See all VEGF Receptor 2 primary antibodies
  • Description
    Rabbit polyclonal to VEGF Receptor 2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IP, IHC-P, IHC-Fr, ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Dog, Human
  • Immunogen

    Synthetic peptide:

    KMVDAAVHADSGTTLQLTSC

    , corresponding to C terminal amino acids 1326-1345 of Mouse VEGF Receptor 2.

  • Positive control
    • Angiosarcoma.
  • General notes

    Isoform 1 localization: Cell membrane; Single-pass type I membrane protein. Cytoplasm. Nucleus. Cytoplasmic vesicle. Early endosome.

    Note: Detected on caveolae-enriched lipid rafts at the cell surface. Is recycled from the plasma membrane to endosomes and back again. Phosphorylation triggered by VEGFA binding promotes internalization and subsequent degradation. VEGFA binding triggers internalization and translocation to the nucleus.

Applications

Our Abpromise guarantee covers the use of ab2349 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/100.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr 1/50 - 1/100.
ICC/IF Use at an assay dependent concentration. See Abreview.
Flow Cyt Use at an assay dependent concentration. PubMed: 18602918

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Function
    Receptor for VEGF or VEGFC. Has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
  • Involvement in disease
    Defects in KDR are associated with susceptibility to hemangioma capillary infantile (HCI) [MIM:602089]. HCI are benign, highly proliferative lesions involving aberrant localized growth of capillary endothelium. They are the most common tumor of infancy, occurring in up to 10% of all births. Hemangiomas tend to appear shortly after birth and show rapid neonatal growth for up to 12 months characterized by endothelial hypercellularity and increased numbers of mast cells. This phase is followed by slow involution at a rate of about 10% per year and replacement by fibrofatty stroma.
  • Sequence similarities
    Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
    Contains 7 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Phosphorylated. Dephosphorylated by PTPRB. Dephosphorylated by PTPRJ at Tyr-951, Tyr-996, Tyr-1054, Tyr-1059, Tyr-1175 and Tyr-1214.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD309 antibody
    • CD309 antigen antibody
    • EC 2.7.10.1 antibody
    • Fetal liver kinase 1 antibody
    • FLK-1 antibody
    • FLK1 antibody
    • FLK1, mouse, homolog of antibody
    • Kdr antibody
    • Kinase insert domain receptor (a type III receptor tyrosine kinase) antibody
    • Kinase insert domain receptor antibody
    • KRD1 antibody
    • Ly73 antibody
    • Protein tyrosine kinase receptor FLK1 antibody
    • Protein-tyrosine kinase receptor flk-1 antibody
    • soluble VEGFR2 antibody
    • Tyrosine kinase growth factor receptor antibody
    • Vascular endothelial growth factor receptor 2 antibody
    • VEGFR 2 antibody
    • VEGFR antibody
    • VEGFR-2 antibody
    • VEGFR2 antibody
    • VGFR2_HUMAN antibody
    see all

Images

  • ab2349 staining the VEGF Receptor 2 in Mouse skin tissue sections by Immunohistochemistry (IHC-P - formaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 hour at room temperature; antigen retrieval was by heat mediation in citrate buffer (pH 6). Samples were incubated with primary antibody (1/100 in PBS + 2% blocking serum) for 16 hours at 4°C. A biotin-conjugated Goat anti-rabbit IgG polyclonal (1/250) was used as the secondary antibody.

    See Abreview

  • Immunocytochemistry/ Immunofluorescence analysis of human cardiomyocytes labeling VEGF Receptor 2 with ab2349 at 1/400 dilution. Cells were fixed with acetone and blocked with 5% serum for 1 hour at 25°C. Cells were incubated with ab2349 at 1/400 for 24 hours at 4°C in 10% FBS. A polyclonal goat anti-rabbit IgG Alexa Fluor® 488 conjugate was used as the secondary antibody at 1/1000 dilution.

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human normal breast tissue labeling VEGF Receptor 2 with ab2349 at 1/200 dilution. Sections were lightly counterstained with hematoxylin.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human normal breast tissue labeling VEGF Receptor 2 with ab2349 at 1/200 dilution. Sections were lightly counterstained with hematoxylin.

  • ab2349 at a 1/100 dilution staining Human choroidal endothelial cells by Immunocytochemistry. The antibody was incubated with the cells for 3 hours and then detected using an Alexa-Fluor 488® goat anti-rabbit (IgG).

    This image is courtesy of an Abreview submitted on 27 February 2006.

    See Abreview

References

This product has been referenced in:
  • Acosta SA  et al. Endothelial Progenitor Cells Modulate Inflammation-Associated Stroke Vasculome. Stem Cell Rev 15:256-275 (2019). Read more (PubMed: 30739275) »
  • Zheng GH  et al. Purple Sweet Potato Color Attenuates Kidney Damage by Blocking VEGFR2/ROS/NLRP3 Signaling in High-Fat Diet-Treated Mice. Oxid Med Cell Longev 2019:5189819 (2019). Read more (PubMed: 30805082) »
See all 80 Publications for this product

Customer reviews and Q&As

Filter by Application

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1-10 of 11 Abreviews

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (First trimester human placenta)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium Citrate
Specification
First trimester human placenta
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Mar 29 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Dog Tissue sections (Granulation tissue)
Antigen retrieval step
Heat mediated
Permeabilization
No
Specification
Granulation tissue
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 30% · Temperature: 25°C
Fixative
Formaldehyde

Francisco Fernandez Flores

Verified customer

Submitted Dec 01 2015

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (iCell® Cardiomyocytes)
Permeabilization
No
Specification
iCell® Cardiomyocytes
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Acetone

Abcam user community

Verified customer

Submitted Nov 25 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Antigen retrieval step
None
Sample
Rat Tissue sections (skin)
Specification
skin
Permeabilization
No
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Jun 10 2014

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (skin)
Specification
skin
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH6
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C

Mr. Manoj Kumar Valluru

Verified customer

Submitted Jun 22 2010

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Skin)
Specification
Skin
Fixative
Zn2+
Antigen retrieval step
None
Permeabilization
No
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 23°C

Abcam user community

Verified customer

Submitted Aug 19 2008

Application
Immunocytochemistry/ Immunofluorescence
Sample
Cow Cell (retinal endothelium)
Specification
retinal endothelium
Fixative
Paraformaldehyde
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5%

Abcam user community

Verified customer

Submitted Mar 01 2006

Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (fibroblasts)
Specification
fibroblasts
Fixative
Paraformaldehyde
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5%

Abcam user community

Verified customer

Submitted Feb 28 2006

Application
Immunocytochemistry/ Immunofluorescence
Sample
Dog Cell (kidney epithelial)
Specification
kidney epithelial
Fixative
Paraformaldehyde
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5%

Abcam user community

Verified customer

Submitted Feb 28 2006

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (retinal pigment epithelium)
Specification
retinal pigment epithelium
Fixative
Paraformaldehyde
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5%

Abcam user community

Verified customer

Submitted Feb 28 2006

1-10 of 11 Abreviews

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