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    vegf-receptor-2-antibody-ab39256.pdf

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Cardiovascular Angiogenesis Growth Factors VEGF VEGF Receptors
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Anti-VEGF Receptor 2 antibody (ab39256)

  • Datasheet
  • SDS
Reviews (8) Submit a question References (54)

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Western blot - Anti-VEGF Receptor 2 antibody (ab39256)

    Key features and details

    • Rabbit polyclonal to VEGF Receptor 2
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    You may also be interested in

    Protein
    Product image
    Recombinant human VEGF Receptor 2 protein (Active) (ab106310)
    Secondary
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    Primary
    Product image
    Anti-VEGF Receptor 1 antibody [Y103] (ab32152)

    View more associated products

    Overview

    • Product name

      Anti-VEGF Receptor 2 antibody
      See all VEGF Receptor 2 primary antibodies
    • Description

      Rabbit polyclonal to VEGF Receptor 2
    • Host species

      Rabbit
    • Tested Applications & Species

      Application Species
      WB
      Human
      See all applications and species data
    • Immunogen

      Synthetic peptide corresponding to Human VEGF Receptor 2 aa 1200-1300 conjugated to keyhole limpet haemocyanin.
      (Peptide available as ab39255)

    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

      One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

      Learn more here.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7.40
      Preservative: 0.02% Sodium azide
      Constituent: PBS

      Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Cardiovascular
      • Angiogenesis
      • Growth Factors
      • VEGF
      • VEGF Receptors
      • Microbiology
      • Interspecies Interaction
      • Host Virus Interaction
      • Signal Transduction
      • Protein Phosphorylation
      • Tyrosine Kinases
      • Receptor Tyrosine Kinases
      • Signal Transduction
      • Growth Factors/Hormones
      • VEGF
      • Stem Cells
      • Hematopoietic Progenitors
      • Surface Molecules
      • Cancer
      • Growth factors
      • VEGF
      • Cancer
      • Invasion/microenvironment
      • Angiogenesis
      • Angiogenic growth factors
      • Cancer
      • Oncoproteins/suppressors
      • Oncoproteins
      • Growth factor receptors
      • Stem Cells
      • Endothelial Progenitors
      • Endothelial Markers
      • Cancer
      • Cancer Metabolism
      • Response to hypoxia
      • Cardiovascular
      • Cardiovascular Markers
      • Cell Markers
      • Endothelial Cells
      • Metabolism
      • Pathways and Processes
      • Metabolism processes
      • Hypoxia
      • Metabolism
      • Types of disease
      • Cancer
      • Cardiovascular
      • Angiogenesis
      • Endothelial Cell Markers

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Immunizing Peptide (Blocking)

      • Human VEGF Receptor 2 peptide (ab39255)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant human VEGF Receptor 2 protein (Active) (ab106310)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab39256 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    WB
    Human
    All applications
    Mouse
    Rat
    Application Abreviews Notes
    WB (5)
    Use a concentration of 1 µg/ml. Detects a band of approximately 220 kDa (predicted molecular weight: 152 kDa).Can be blocked with Human VEGF Receptor 2 peptide (ab39255).
    Notes
    WB
    Use a concentration of 1 µg/ml. Detects a band of approximately 220 kDa (predicted molecular weight: 152 kDa).Can be blocked with Human VEGF Receptor 2 peptide (ab39255).

    Target

    • Function

      Receptor for VEGF or VEGFC. Has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
    • Involvement in disease

      Defects in KDR are associated with susceptibility to hemangioma capillary infantile (HCI) [MIM:602089]. HCI are benign, highly proliferative lesions involving aberrant localized growth of capillary endothelium. They are the most common tumor of infancy, occurring in up to 10% of all births. Hemangiomas tend to appear shortly after birth and show rapid neonatal growth for up to 12 months characterized by endothelial hypercellularity and increased numbers of mast cells. This phase is followed by slow involution at a rate of about 10% per year and replacement by fibrofatty stroma.
    • Sequence similarities

      Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
      Contains 7 Ig-like C2-type (immunoglobulin-like) domains.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications

      Phosphorylated. Dephosphorylated by PTPRB. Dephosphorylated by PTPRJ at Tyr-951, Tyr-996, Tyr-1054, Tyr-1059, Tyr-1175 and Tyr-1214.
    • Cellular localization

      Membrane.
    • Target information above from: UniProt accession P35968 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 3791 Human
      • Entrez Gene: 16542 Mouse
      • Entrez Gene: 25589 Rat
      • Omim: 191306 Human
      • SwissProt: P35968 Human
      • SwissProt: P35918 Mouse
      • SwissProt: O08775 Rat
      • Unigene: 479756 Human
      • Unigene: 285 Mouse
      • Unigene: 88869 Rat
      see all
    • Alternative names

      • CD309 antibody
      • CD309 antigen antibody
      • EC 2.7.10.1 antibody
      • Fetal liver kinase 1 antibody
      • FLK-1 antibody
      • FLK1 antibody
      • FLK1, mouse, homolog of antibody
      • Kdr antibody
      • Kinase insert domain receptor (a type III receptor tyrosine kinase) antibody
      • Kinase insert domain receptor antibody
      • KRD1 antibody
      • Ly73 antibody
      • Protein tyrosine kinase receptor FLK1 antibody
      • Protein-tyrosine kinase receptor flk-1 antibody
      • soluble VEGFR2 antibody
      • Tyrosine kinase growth factor receptor antibody
      • Vascular endothelial growth factor receptor 2 antibody
      • VEGFR 2 antibody
      • VEGFR antibody
      • VEGFR-2 antibody
      • VEGFR2 antibody
      • VGFR2_HUMAN antibody
      see all

    Images

    • Western blot - Anti-VEGF Receptor 2 antibody (ab39256)
      Western blot - Anti-VEGF Receptor 2 antibody (ab39256)
      Anti-VEGF Receptor 2 antibody (ab39256) at 1 µg/ml + HUVEC (Human Umbilical Vein Endothelial Cell) Whole Cell Lysate at 20 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

      Predicted band size: 152 kDa
      Observed band size: 220,230 kDa
      why is the actual band size different from the predicted?
      Additional bands at: 31 kDa. We are unsure as to the identity of these extra bands.

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (54)

    Publishing research using ab39256? Please let us know so that we can cite the reference in this datasheet.

    ab39256 has been referenced in 54 publications.

    • Cho YR  et al. A novel role for a-viniferin in suppressing angiogenesis by blocking the VEGFR-2/p70S6K signaling pathway. Phytother Res N/A:N/A (2020). PubMed: 32400050
    • Gao C  et al. MALAT1 Protected the Angiogenesis Function of Human Brain Microvascular Endothelial Cells (HBMECs) Under Oxygen Glucose Deprivation/re-oxygenation (OGD/R) Challenge by Interacting with miR-205-5p/VEGFA Pathway. Neuroscience 435:135-145 (2020). PubMed: 32217110
    • Hassanpour M  et al. Autophagy modulation altered differentiation capacity of CD146+ cells toward endothelial cells, pericytes, and cardiomyocytes. Stem Cell Res Ther 11:139 (2020). PubMed: 32216836
    • Besnier M  et al. miR-15a/-16 Inhibit Angiogenesis by Targeting the Tie2 Coding Sequence: Therapeutic Potential of a miR-15a/16 Decoy System in Limb Ischemia. Mol Ther Nucleic Acids 17:49-62 (2019). PubMed: 31220779
    • Jiao W  et al. Distinct downstream signaling and the roles of VEGF and PlGF in high glucose-mediated injuries of human retinal endothelial cells in culture. Sci Rep 9:15339 (2019). PubMed: 31653890
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review

    Filter by Application

    Filter by Species

    Filter by Ratings

    1-8 of 8 Abreviews

    Western blot abreview for Anti-VEGF Receptor 2 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Sheep Tissue lysate - whole (caruncle tissue)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    50 µg
    Specification
    caruncle tissue
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Mrs. Gail Silver

    Verified customer

    Submitted Aug 09 2019

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-VEGF Receptor 2 antibody

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rabbit Tissue sections (FFPE rabbit joint section)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: ventana CC1(EDTA)
    Permeabilization
    No
    Specification
    FFPE rabbit joint section
    Fixative
    10% neutral formalin
    Read More

    Abcam user community

    Verified customer

    Submitted Apr 13 2017

    Western blot abreview for Anti-VEGF Receptor 2 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Sheep Tissue lysate - whole (Lung)
    Gel Running Conditions
    Reduced Denaturing (7.5%)
    Loading amount
    20 µg
    Specification
    Lung
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More

    Miss. Maryline Gomes

    Verified customer

    Submitted Jul 08 2015

    Western blot abreview for Anti-VEGF Receptor 2 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    50 µg
    Gel Running Conditions
    Reduced Denaturing (5% gel)
    Sample
    Cow Tissue lysate - other (Corpus luteum)
    Specification
    Corpus luteum
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 24°C
    Read More

    Sir or Madam ??

    Verified customer

    Submitted Jan 23 2015

    Western blot abreview for Anti-VEGF Receptor 2 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    25 µg
    Gel Running Conditions
    Reduced Denaturing
    Sample
    Human Cell lysate - whole cell (Hela, Hep G2)
    Specification
    Hela, Hep G2
    Treatment
    Na3VO4 (sodium orthovanadate) 0.3nM 1 h
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jan 02 2015

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-VEGF Receptor 2 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Breast carcinoma)
    Specification
    Breast carcinoma
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Dako Target Retreival Solution
    Permeabilization
    No
    Blocking step
    5 minutes of peroxidase block then 10 minutes of protein block. These are ready-to-use reagents purchased from Dako as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C
    Read More

    Mr. Antibody Solutions

    Verified customer

    Submitted Mar 18 2010

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-VEGF Receptor 2 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Rat Tissue sections (Heart)
    Specification
    Heart
    Fixative
    Paraformaldehyde
    Antigen retrieval step
    None
    Permeabilization
    No
    Read More

    Dr. Sophie Pezet

    Verified customer

    Submitted Jul 31 2009

    Western blot abreview for Anti-VEGF Receptor 2 antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (HEK293 U251 Ms Brain PC12)
    Loading amount
    30 µg
    Specification
    HEK293 U251 Ms Brain PC12
    Gel Running Conditions
    Reduced Denaturing (7.5% SDS-PAGE)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Read More

    Mr. Antibody Solutions

    Verified customer

    Submitted Sep 03 2008

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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