Recombinant
RabMAb

Recombinant Anti-VEGF Receptor 2 antibody [EPR21884-236] - BSA and Azide free (ab234106)

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Overview

  • Product name

    Anti-VEGF Receptor 2 antibody [EPR21884-236] - BSA and Azide free
    See all VEGF Receptor 2 primary antibodies

  • Description

    Rabbit monoclonal [EPR21884-236] to VEGF Receptor 2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse

  • Immunogen

    Recombinant fragment within Mouse VEGF Receptor 2 aa 1-800. The exact sequence is proprietary.
    Database link: P35918

  • Positive control

    • ICC/IF: bEnd.3 cells.

  • General notes

    Ab234106 is the carrier-free version of ab233693. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab234106 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Applications

Our Abpromise guarantee covers the use of ab234106 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

Target

  • Function

    Receptor for VEGF or VEGFC. Has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.

  • Involvement in disease

    Defects in KDR are associated with susceptibility to hemangioma capillary infantile (HCI) [MIM:602089]. HCI are benign, highly proliferative lesions involving aberrant localized growth of capillary endothelium. They are the most common tumor of infancy, occurring in up to 10% of all births. Hemangiomas tend to appear shortly after birth and show rapid neonatal growth for up to 12 months characterized by endothelial hypercellularity and increased numbers of mast cells. This phase is followed by slow involution at a rate of about 10% per year and replacement by fibrofatty stroma.

  • Sequence similarities

    Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
    Contains 7 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.

  • Post-translational
    modifications

    Phosphorylated. Dephosphorylated by PTPRB. Dephosphorylated by PTPRJ at Tyr-951, Tyr-996, Tyr-1054, Tyr-1059, Tyr-1175 and Tyr-1214.

  • Cellular localization

    Membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • CD309 antibody
      • CD309 antigen antibody
      • EC 2.7.10.1 antibody
      • Fetal liver kinase 1 antibody
      • FLK-1 antibody
      • FLK1 antibody
      • FLK1, mouse, homolog of antibody
      • Kdr antibody
      • Kinase insert domain receptor (a type III receptor tyrosine kinase) antibody
      • Kinase insert domain receptor antibody
      • KRD1 antibody
      • Ly73 antibody
      • Protein tyrosine kinase receptor FLK1 antibody
      • Protein-tyrosine kinase receptor flk-1 antibody
      • soluble VEGFR2 antibody
      • Tyrosine kinase growth factor receptor antibody
      • Vascular endothelial growth factor receptor 2 antibody
      • VEGFR 2 antibody
      • VEGFR antibody
      • VEGFR-2 antibody
      • VEGFR2 antibody
      • VGFR2_HUMAN antibody
      see all

    Images

    • Flow Cytometry - Anti-VEGF Receptor 2 antibody [EPR21884-236] - BSA and Azide free (ab234106)
      Flow Cytometry - Anti-VEGF Receptor 2 antibody [EPR21884-236] - BSA and Azide free (ab234106)

      Flow cytometric analysis of bEnd.3 (mouse brain endothelioma cell line) cell line treated with 1 μg/ml Brefeldin A for 3 hours (red) and an untreated control (green) labeling VEGF Receptor 2 with ab233693 at 1/500 dilution treated with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

      BFA treatment reduced cell surface VEGF Receptor 2 [PMID: 21063020].

      Gated on viable cells.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab233693).

    • Flow Cytometry - Anti-VEGF Receptor 2 antibody [EPR21884-236] - BSA and Azide free (ab234106)
      Flow Cytometry - Anti-VEGF Receptor 2 antibody [EPR21884-236] - BSA and Azide free (ab234106)

      Flow cytometric analysis of bEnd.3 (mouse brain endothelioma cell line, right) and NIH/3T3 (mouse embryo fibroblast cell line, left) cell line labeling VEGF Receptor 2 with ab233693 at 1/500 dilution compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

      Gated on viable cells.

      Negative control: NIH/3T3 [PMID: 20978347].

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab233693).

    • Immunocytochemistry/ Immunofluorescence - Anti-VEGF Receptor 2 antibody [EPR21884-236] - BSA and Azide free (ab234106)
      Immunocytochemistry/ Immunofluorescence - Anti-VEGF Receptor 2 antibody [EPR21884-236] - BSA and Azide free (ab234106)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized bEnd.3 (mouse brain endothelioma) cells labeling VEGF Receptor 2 with ab233693 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Redistribution of the VEGF Receptor 2 from the perinuclear Golgi to vesicular structures throughout the cytosol after treatment with Brefeldin A (1 μg/ml, 3 hours) in bEnd.3. BFA treatment alters the subcellular localisation of VEGF Receptor 2 [PMID: 21063020].

      The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab233693).

    • Immunocytochemistry/ Immunofluorescence - Anti-VEGF Receptor 2 antibody [EPR21884-236] - BSA and Azide free (ab234106)
      Immunocytochemistry/ Immunofluorescence - Anti-VEGF Receptor 2 antibody [EPR21884-236] - BSA and Azide free (ab234106)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized bEnd.3 (mouse brain endothelioma cell line) and NIH/3T3 (mouse embryo fibroblast cell line) cells labeling VEGF Receptor 2 with ab233693 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing perinuclear staining in bEnd.3 cells.

      Negative control: NIH/3T3  [PMID: 20978347].

      The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab233693).

    • Immunocytochemistry/ Immunofluorescence - Anti-VEGF Receptor 2 antibody [EPR21884-236] - BSA and Azide free (ab234106)
      Immunocytochemistry/ Immunofluorescence - Anti-VEGF Receptor 2 antibody [EPR21884-236] - BSA and Azide free (ab234106)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized bEnd.3 (mouse brain endothelioma cell line) and NIH/3T3 (mouse embryo fibroblast cell line) cells labeling VEGF Receptor 2 with ab233693 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing perinuclear staining in bEnd.3 cells.

      Negative control: NIH/3T3  [PMID: 20978347].

      The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab233693).

       

    References

    ab234106 has not yet been referenced specifically in any publications.

    Publishing research using ab234106? Please let us know so that we can cite the reference in this datasheet.

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