Overview

  • Product name
    Anti-VEGF Receptor 2 antibody [mV1001.3m-h]
    See all VEGF Receptor 2 primary antibodies
  • Description
    Mouse monoclonal [mV1001.3m-h] to VEGF Receptor 2
  • Host species
    Mouse
  • Tested applications
    Suitable for: Sandwich ELISA, WB, IP, Functional Studiesmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Purified fragment, corresponding to N terminal amino acids 30-200 of Human VEGF Receptor 2

  • Positive control
    • Human Umbilical Vein Endothelial Cells (HUVECs).

Applications

Our Abpromise guarantee covers the use of ab42228 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use a concentration of 5 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to VEGF Receptor 2 (ab11939).

For sandwich ELISA, use this antibody as Capture at 5µg/ml with ab11939 as Detection.

WB Use at an assay dependent concentration. Predicted molecular weight: 151 kDa.
IP Use at an assay dependent concentration.
Functional Studies Use a concentration of 5 - 25 µg/ml. This antibody can induce VEGF Receptor 2 tyrosine phosphorylation in Human Umbilical Vein Endothelial Cells (HUVECs).

Target

  • Function
    Receptor for VEGF or VEGFC. Has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
  • Involvement in disease
    Defects in KDR are associated with susceptibility to hemangioma capillary infantile (HCI) [MIM:602089]. HCI are benign, highly proliferative lesions involving aberrant localized growth of capillary endothelium. They are the most common tumor of infancy, occurring in up to 10% of all births. Hemangiomas tend to appear shortly after birth and show rapid neonatal growth for up to 12 months characterized by endothelial hypercellularity and increased numbers of mast cells. This phase is followed by slow involution at a rate of about 10% per year and replacement by fibrofatty stroma.
  • Sequence similarities
    Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
    Contains 7 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Phosphorylated. Dephosphorylated by PTPRB. Dephosphorylated by PTPRJ at Tyr-951, Tyr-996, Tyr-1054, Tyr-1059, Tyr-1175 and Tyr-1214.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD309 antibody
    • CD309 antigen antibody
    • EC 2.7.10.1 antibody
    • Fetal liver kinase 1 antibody
    • FLK-1 antibody
    • FLK1 antibody
    • FLK1, mouse, homolog of antibody
    • Kdr antibody
    • Kinase insert domain receptor (a type III receptor tyrosine kinase) antibody
    • Kinase insert domain receptor antibody
    • KRD1 antibody
    • Ly73 antibody
    • Protein tyrosine kinase receptor FLK1 antibody
    • Protein-tyrosine kinase receptor flk-1 antibody
    • soluble VEGFR2 antibody
    • Tyrosine kinase growth factor receptor antibody
    • Vascular endothelial growth factor receptor 2 antibody
    • VEGFR 2 antibody
    • VEGFR antibody
    • VEGFR-2 antibody
    • VEGFR2 antibody
    • VGFR2_HUMAN antibody
    see all

Images

  • Standard curve for VEGF Receptor 2 (Analyte: ab54347); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [mV1001.3m-h] to VEGF Receptor 2 (ab42228) at 5µg/ml and Detector Antibody Rabbit polyclonal to VEGF Receptor 2 (ab11939) at 0.5µg/ml.
  • Lane 1 : unstimulated cells
    Lane 2 : hVEGF at 20 ng/ml
    Lane 3 : Anti-VEGF Receptor 2 antibody [mV1001.3m-h] (ab42228) at 5 µg/ml
    Lane 4 : Anti-VEGF Receptor 2 antibody [mV1001.3m-h] (ab42228) at 25 µg/ml

    All lanes : HUVECs

    Predicted band size: 151 kDa
    Observed band size: 220 kDa
    why is the actual band size different from the predicted?



    HUVECs (human umbilical vein endothelial cells) were stimulated with hVEGF or ab42228 for 30min. Phospho-VEGF Receptor 2 was detected with IP-Western for Phosphotyrosine.

References

This product has been referenced in:
  • Ptaszynska MM  et al. Positive Feedback between Vascular Endothelial Growth Factor-A and Autotaxin in Ovarian Cancer Cells. Mol Cancer Res 6:352-63 (2008). Read more (PubMed: 18337445) »
See 1 Publication for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Answer

Thank you for your reply. I have now returned to the office an hope I can help further with your enquiry.

I can now confirm that of the antibodies we have directed against VEGF Receptor 2 (that are not raised in rabbit), most likely to react with the sheep protein are the mouse monoclonals ab10972, ab10973 and ab82266. This is based on the immunogen used to raise these antibodies. They have all be raised against the extracellular domain of the VEGF Receptor 2, however, this region covers the residues 1-745 and as I only have the region between 230-400 of the sheep protein to compare I cannot be at all certain that they will work, as you have observed with ab10972. We have ourselves not tested any of these antibodies with samples from sheep and therefore cannot guarantee their performance.

If you would like, I can offer a testing discount with ab10973 or ab82266. More information on this offer can be found from the following link:

www.abcam.com/abtrial

However, I would suggest the rabbit antibodies I suggested, ab39521, ab62697, ab63613 and ab131510 (especially ab131510 as it has been used in ICC/IF as well as staining of paraffin embedded sections) would be more likely to work with your samples.

Again, if you would like to participate in the Abtrial with any of these antibodies please do let me know.

I hope this information has been of some help. If I can be of any further assistance, please do not hesitate to let me know.

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Answer



Reviewing the correspondence on this enquiry so far, I notice xxxx has selected some antibodies not raised in rabbit and has been in contact with the originators to obtain further information regarding the immunogen sequence so we can work out whether they would detect the two fragments of sequence previously discussed (from SwissProt (Q8MI25 and Q8MI23) align to ˜230-400 and 1020-1200 of human and mouse VEGF Receptor 2 respectively).

I apologise for the delay so far. We are waiting for one more reply. However, the most promising response we have had is regarding ab10972 Anti-VEGF Receptor 2 antibody. The immunogen used to raise this product was purified recombinant mouse VEGF R2 extracellular domain expressed in NSO cells. The human extracellular domain (soluble) corresponds to residues 1-745. We do not know for sure whether these are the exact mouse protein residues in the immunising sequence but it is likely to cover residues 230-400 that has been specified in previous emails.

Please note that regrettably we are not able to guarantee this antibody for Sheep as it has not been tested. The applications it has been tested and guaranteed for are ICC/IF, Indirect ELISA, IHC-P, Flow Cyt, ICC, WB, Neutralising, Blocking.

If you would like to test this antibody in sheep or an untested application, please contact me again by replying to this message prior to the purchase as you may be eligible for our AbTrial testing discount program.
Otherwise, we like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates.


To find out more about our Abreview system, please see the following link:

https://www.abcam.com/abreviews

I am sorry we do not have a suitably tested antibody to suggest on this occasion. However, I hope this information is helpful. Please do not hesitate to contact me if you have any further questions or concerns.

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Question
Answer

Thank you for contacting us.

I have checked with the lab and they did not try using lysate for the activation assay but are in agreement that this would probably not work due to the presence of detergents and lysis buffer.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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