Key features and details
- Rabbit polyclonal to VEGF Receptor 2 (phospho Y1054 + Y1059)
- Suitable for: ICC, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-VEGF Receptor 2 (phospho Y1054 + Y1059) antibody
See all VEGF Receptor 2 primary antibodies
DescriptionRabbit polyclonal to VEGF Receptor 2 (phospho Y1054 + Y1059)
Tested applicationsSuitable for: ICC, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide corresponding to Human VEGF Receptor 2 (phospho Y1054 + Y1059).
- ICC: VEGF treated HUVEC whole cells. WB: VEGF treated HUVEC, MDA-MB-231 and MCF7 whole cell extract.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.1% BSA, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThe antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated VEGFR 2. The final product is generated by affinity chromatography using a VEGFR 2 derived peptide that is phosphorylated at Tyrosines 1054 and 1059.
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab5473 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000. Detects a band of approximately 200 kDa.
1/1000. Detects a band of approximately 200 kDa.
FunctionReceptor for VEGF or VEGFC. Has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
Involvement in diseaseDefects in KDR are associated with susceptibility to hemangioma capillary infantile (HCI) [MIM:602089]. HCI are benign, highly proliferative lesions involving aberrant localized growth of capillary endothelium. They are the most common tumor of infancy, occurring in up to 10% of all births. Hemangiomas tend to appear shortly after birth and show rapid neonatal growth for up to 12 months characterized by endothelial hypercellularity and increased numbers of mast cells. This phase is followed by slow involution at a rate of about 10% per year and replacement by fibrofatty stroma.
Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
Contains 7 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain.
modificationsPhosphorylated. Dephosphorylated by PTPRB. Dephosphorylated by PTPRJ at Tyr-951, Tyr-996, Tyr-1054, Tyr-1059, Tyr-1175 and Tyr-1214.
- Information by UniProt
- CD309 antibody
- CD309 antigen antibody
- EC 126.96.36.199 antibody
Immunofluorescence analysis of HUVEC cells labelling VEGF Receptor 2 (phospho Y1054 + Y1059) (PAnel a: green) using ab5473 at 2 ug/ml in 0.1% BSA for 3 hours at room temperature, follwed by Alexa Fluor® 488-conjugated Goat anti-rabbit IgG polyclonal at 1/2000 dilution. Pbel b: Nuclei stained with DAPI (blue), Panel c: F-actin stained with Alexa Fluor® 555 Rhodamine Phalloidin (red), Panel d: Merged images. The images were captured at 60X magnification.
Prior antibody incubation, HUVEC (Human umbilical vein endothelial cell line) cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes and blocked with 1% BSA for 1 hour at room temperature, followed by treatment with 100 ng of VEGF for 30 minutes. Assay was done on 90% confluent log phase HUVEC cells.
All lanes : Anti-VEGF Receptor 2 (phospho Y1054 + Y1059) antibody (ab5473) at 1/1000 dilution
Lane 1 : HUVEC (Human umbilical vein endothelial cell line) whole cell extract with Skimmed milk
Lane 2 : HUVEC cells treated with 25ng/ml VEGF for 5 minutes with Skimmed milk
Lane 3 : MDA-MB-231 (human breast adenocarcinoma cell line) whole cell extract with Skimmed milk
Lane 4 : MCF7 (human breast adenocarcinoma cell line) whole cell extract with Skimmed milk
Lysates/proteins at 30 µg per lane.
Blocking peptides at 5 % per lane.
All lanes : Goat anti-rabbit IgG HRP conjugate at 1/5000 dilution
A 130 kDa band corresponding to VEGFR (pYpY1054/1059) was observed across cell lines tested.
Protein samples were electrophoresed using Novex® NuPAGE® 10 % Bis-Tris gel, XCell SureLock™ Electrophoresis System and Novex® Sharp Pre-Stained Protein Standard.
Resolved proteins were then transferred onto a nitrocellulose membrane by over night wet transfer.
Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate.
ab5473 has been referenced in 30 publications.
- Li L et al. Brivanib, a multitargeted small-molecule tyrosine kinase inhibitor, suppresses laser-induced CNV in a mouse model of neovascular AMD. J Cell Physiol 235:1259-1273 (2020). PubMed: 31270802
- Cho YR et al. A novel role for a-viniferin in suppressing angiogenesis by blocking the VEGFR-2/p70S6K signaling pathway. Phytother Res N/A:N/A (2020). PubMed: 32400050
- Jin B et al. Yangjing Capsule Can Improve the Function of the Testicular Angiogenesis through Activating VEGFA/eNOS Signaling Pathway. Evid Based Complement Alternat Med 2020:1957267 (2020). PubMed: 32382279
- Bradley JR et al. Tumor necrosis factor receptor-2 signaling pathways promote survival of cancer stem-like CD133+ cells in clear cell renal carcinoma. FASEB Bioadv 2:126-144 (2020). PubMed: 32123862
- Noh JJ et al. Anti-Cancer Activity of As4O6 and its Efficacy in a Series of Patient-Derived Xenografts for Human Cervical Cancer. Pharmaceutics 12:N/A (2020). PubMed: 33086573