• Product name
    Anti-VEGF Receptor 2 (phospho Y1054 + Y1059) antibody
    See all VEGF Receptor 2 primary antibodies
  • Description
    Rabbit polyclonal to VEGF Receptor 2 (phospho Y1054 + Y1059)
  • Host species
  • Tested applications
    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic phospho peptide (Human)containing Tyrosines 1054 and 1059. This sequence is conserved in mouse and rat.

  • Positive control
    • Porcine aortic endothelial cells transfected with a chimeric receptor consisting of the extracellular portion of the CSF 1 receptor fused with the transmembrane and intracellular domains of mouse VEGFR 2.



Our Abpromise guarantee covers the use of ab5473 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/250.
IHC-P Use at an assay dependent concentration. PubMed: 20566746
WB Use a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 200 kDa.


  • Function
    Receptor for VEGF or VEGFC. Has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
  • Involvement in disease
    Defects in KDR are associated with susceptibility to hemangioma capillary infantile (HCI) [MIM:602089]. HCI are benign, highly proliferative lesions involving aberrant localized growth of capillary endothelium. They are the most common tumor of infancy, occurring in up to 10% of all births. Hemangiomas tend to appear shortly after birth and show rapid neonatal growth for up to 12 months characterized by endothelial hypercellularity and increased numbers of mast cells. This phase is followed by slow involution at a rate of about 10% per year and replacement by fibrofatty stroma.
  • Sequence similarities
    Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
    Contains 7 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.
  • Post-translational
    Phosphorylated. Dephosphorylated by PTPRB. Dephosphorylated by PTPRJ at Tyr-951, Tyr-996, Tyr-1054, Tyr-1059, Tyr-1175 and Tyr-1214.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • CD309 antibody
    • CD309 antigen antibody
    • EC antibody
    • Fetal liver kinase 1 antibody
    • FLK-1 antibody
    • FLK1 antibody
    • FLK1, mouse, homolog of antibody
    • Kdr antibody
    • Kinase insert domain receptor (a type III receptor tyrosine kinase) antibody
    • Kinase insert domain receptor antibody
    • KRD1 antibody
    • Ly73 antibody
    • Protein tyrosine kinase receptor FLK1 antibody
    • Protein-tyrosine kinase receptor flk-1 antibody
    • soluble VEGFR2 antibody
    • Tyrosine kinase growth factor receptor antibody
    • Vascular endothelial growth factor receptor 2 antibody
    • VEGFR 2 antibody
    • VEGFR antibody
    • VEGFR-2 antibody
    • VEGFR2 antibody
    • VGFR2_HUMAN antibody
    see all


  • All lanes : Anti-VEGF Receptor 2 (phospho Y1054 + Y1059) antibody (ab5473) at 1/100 dilution

    Lane 1 : Human umbilical vein endothelial cells with Skimmed milk
    Lane 2 : Human umbilical vein endothelial cells treated with 25ng/ml VEGF with Skimmed milk
    Lane 3 : MDA-MB-231 with Skimmed milk
    Lane 4 : MCF7 with Skimmed milk

    Lysates/proteins at 30 µg per lane.

    Blocking peptides at 5 % per lane.

    All lanes : HRP conjugated Goat anti-rabbit IgG at 1/5000 dilution
  • ab5473 staining VEGF Receptor 2 (phospho Y1054 + Y1059) in 90% confluent log phase Human umbilical vein endothelial cells (panel a) by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 1% BSA for 1 hour at room temperature. Samples were incubated with primary antibody (2 ug/ml in 0.1% BSA) for 3 hours at room temperature. An Alexa Fluor® 488-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody (1/2000). Nuclei stained blue with DAPI (panel b), F-actin stained red with Alexa Fluor® 555 Rhodamine Phalloidin (panel c), merged image shown in panel d.

  • Peptide Competition and Stimulation: Extracts prepared from PAE cells transfected with a chimeric receptor (see Positive Controls section above for full description) were left unstimulated (1, 2) or stimulated with VEGF (3-8), then were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4°C, then were incubated with a VEGFR2 pan antibody (1, 3) or 0.50 µg/mL VEGFR2 ab5473 antibody for two hours at room temperature in a 3% BSA-TBST buffer, following prior incubation with: no peptide (2, 4, 5), the non-phosphopeptide corresponding to the immunogen (6), a generic phosphotyrosine containing peptide (7), or, the phosphopeptide immunogen (8). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG alkaline phosphatase and bands were detected using the Tropix WesternStar method. The data show that only the peptide corresponding to ab5473 blocks the antibody signal,


This product has been referenced in:
  • Jia T  et al. Heteromultivalent targeting of integrin avß3 and neuropilin 1 promotes cell survival via the activation of the IGF-1/insulin receptors. Biomaterials 155:64-79 (2018). Read more (PubMed: 29169039) »
  • Li X  et al. Helios expression in regulatory T cells promotes immunosuppression, angiogenesis and the growth of leukemia cells in pediatric acute lymphoblastic leukemia. Leuk Res 67:60-66 (2018). Read more (PubMed: 29455107) »
See all 16 Publications for this product

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1-2 of 2 Abreviews

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Mouse Tissue sections (Heart)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer, pH6.0
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C

Mr. Mc Shen

Verified customer

Submitted Dec 20 2016

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate bufer
Rat Tissue sections (rat bone marrow tissue)
rat bone marrow tissue

Abcam user community

Verified customer

Submitted Mar 07 2014


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