Key features and details
- Rabbit polyclonal to VEGF Receptor 2 (phospho Y1175)
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-VEGF Receptor 2 (phospho Y1175) antibody
See all VEGF Receptor 2 primary antibodies
DescriptionRabbit polyclonal to VEGF Receptor 2 (phospho Y1175)
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to Human VEGF Receptor 2 (phospho Y1175). A phospho specific peptide corresponding to residues surrounding Y1175 of Human VEGF Receptor 2.
Database link: P35968
- WB: HUVEC treated with VEGF cell lysate; IHC-P: Human breast carcinoma tissue; ICC/IF: MCF-7 cells.
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We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab194806 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/100 - 1/200.|
|IHC-P||1/50 - 1/100.|
|WB||1/500 - 1/1000. Detects a band of approximately 250 kDa (predicted molecular weight: 152 kDa).|
FunctionReceptor for VEGF or VEGFC. Has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
Involvement in diseaseDefects in KDR are associated with susceptibility to hemangioma capillary infantile (HCI) [MIM:602089]. HCI are benign, highly proliferative lesions involving aberrant localized growth of capillary endothelium. They are the most common tumor of infancy, occurring in up to 10% of all births. Hemangiomas tend to appear shortly after birth and show rapid neonatal growth for up to 12 months characterized by endothelial hypercellularity and increased numbers of mast cells. This phase is followed by slow involution at a rate of about 10% per year and replacement by fibrofatty stroma.
Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
Contains 7 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain.
modificationsPhosphorylated. Dephosphorylated by PTPRB. Dephosphorylated by PTPRJ at Tyr-951, Tyr-996, Tyr-1054, Tyr-1059, Tyr-1175 and Tyr-1214.
- Information by UniProt
- CD309 antibody
- CD309 antigen antibody
- EC 126.96.36.199 antibody
All lanes : Anti-VEGF Receptor 2 (phospho Y1175) antibody (ab194806)
Lane 1 : HUVEC treated with VEGF cell lysate
Lane 2 : Untreated HUVEC cell lysate
Lysates/proteins at 25 µg per lane.
All lanes : Goat Anti-rabbit IgG H+L (HRP) at 1/10000 dilution
Predicted band size: 152 kDa
Observed band size: 250 kDa why is the actual band size different from the predicted?
Blocking buffer: 3% BSA
Immunofluorescence analysis of methanol-fixed MCF cells, labeling VEGF Receptor 2 (phospho Y1175) (red) with ab194806 at 1/100 dilution.
Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue, labeling VEGF Receptor 2 (phospho Y1175) with ab194806 at 1/50 dilution.
ab194806 has been referenced in 4 publications.
- Wen S et al. Apatinib regulates the cell proliferation and apoptosis of liver cancer by regulation of VEGFR2/STAT3 signaling. Pathol Res Pract 215:816-821 (2019). PubMed: 30686547
- Thirunavukkarasu M et al. Disruption of VEGF Mediated Flk-1 Signaling Leads to a Gradual Loss of Vessel Health and Cardiac Function During Myocardial Infarction: Potential Therapy With Pellino-1. J Am Heart Assoc 7:e007601 (2018). PubMed: 30371196
- Chen X et al. miR-150-5p suppresses tumor progression by targeting VEGFA in colorectal cancer. Aging (Albany NY) 10:3421-3437 (2018). PubMed: 30476901
- Farzaneh Behelgardi M et al. A peptide mimicking the binding sites of VEGF-A and VEGF-B inhibits VEGFR-1/-2 driven angiogenesis, tumor growth and metastasis. Sci Rep 8:17924 (2018). PubMed: 30560942