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Cardiovascular Angiogenesis Growth Factors VEGF VEGF Receptors
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Anti-VEGF Receptor 2 (phospho Y1214) antibody (ab5475)

  • Datasheet
Submit a review Q&A (1)References (9)

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Western blot - Anti-VEGF Receptor 2 (phospho Y1214) antibody (ab5475)

    Key features and details

    • Rabbit polyclonal to VEGF Receptor 2 (phospho Y1214)
    • Suitable for: WB
    • Reacts with: Recombinant fragment
    • Isotype: IgG

    You may also be interested in

    Protein
    Product image
    Recombinant human VEGF Receptor 2 protein (Active) (ab106310)
    Secondary
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

    View more associated products

    Overview

    • Product name

      Anti-VEGF Receptor 2 (phospho Y1214) antibody
      See all VEGF Receptor 2 primary antibodies
    • Description

      Rabbit polyclonal to VEGF Receptor 2 (phospho Y1214)
    • Host species

      Rabbit
    • Specificity

      VEGFR3 has not been tested, but is expected to react.
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Recombinant fragment
      Predicted to work with: Rat
    • Immunogen

      Synthetic peptide (Human). The sequence is conserved in mouse and rat.

    • Positive control

      • NIH 3T3 cells transfected with full length, wild-type human VEGFR 2 and Porcine Aortic Endothelial cells transfected with a chimeric receptor consisting of the extracellular domain of the CSF 1 receptor coupled to the transmembrane and cytoplasmic domains of the mouse VEGFR 2.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
    • Storage buffer

      pH: 7.3
      Preservative: 0.05% Sodium azide
      Constituents: PBS, 0.1% BSA

      BSA is IgG and protease free
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Purification notes

      The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated VEGFR 2 protein. The final product is generated by affinity chromatography using a VEGFR 2 derived peptide that is phosphorylated at Tyrosine 1214.
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Cardiovascular
      • Angiogenesis
      • Growth Factors
      • VEGF
      • VEGF Receptors
      • Microbiology
      • Interspecies Interaction
      • Host Virus Interaction
      • Signal Transduction
      • Protein Phosphorylation
      • Tyrosine Kinases
      • Receptor Tyrosine Kinases
      • Signal Transduction
      • Growth Factors/Hormones
      • VEGF
      • Stem Cells
      • Hematopoietic Progenitors
      • Surface Molecules
      • Cancer
      • Growth factors
      • VEGF
      • Cancer
      • Invasion/microenvironment
      • Angiogenesis
      • Angiogenic growth factors
      • Cancer
      • Oncoproteins/suppressors
      • Oncoproteins
      • Growth factor receptors
      • Stem Cells
      • Endothelial Progenitors
      • Endothelial Markers
      • Cancer
      • Cancer Metabolism
      • Response to hypoxia
      • Cardiovascular
      • Cardiovascular Markers
      • Cell Markers
      • Endothelial Cells
      • Metabolism
      • Pathways and Processes
      • Metabolism processes
      • Hypoxia
      • Metabolism
      • Types of disease
      • Cancer
      • Cardiovascular
      • Angiogenesis
      • Endothelial Cell Markers

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant human VEGF Receptor 2 protein (Active) (ab106310)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab5475 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB
    Use a concentration of 0.35 - 1 µg/ml. Detects a band of approximately 200 kDa.
    Notes
    WB
    Use a concentration of 0.35 - 1 µg/ml. Detects a band of approximately 200 kDa.

    Target

    • Function

      Receptor for VEGF or VEGFC. Has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
    • Involvement in disease

      Defects in KDR are associated with susceptibility to hemangioma capillary infantile (HCI) [MIM:602089]. HCI are benign, highly proliferative lesions involving aberrant localized growth of capillary endothelium. They are the most common tumor of infancy, occurring in up to 10% of all births. Hemangiomas tend to appear shortly after birth and show rapid neonatal growth for up to 12 months characterized by endothelial hypercellularity and increased numbers of mast cells. This phase is followed by slow involution at a rate of about 10% per year and replacement by fibrofatty stroma.
    • Sequence similarities

      Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
      Contains 7 Ig-like C2-type (immunoglobulin-like) domains.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications

      Phosphorylated. Dephosphorylated by PTPRB. Dephosphorylated by PTPRJ at Tyr-951, Tyr-996, Tyr-1054, Tyr-1059, Tyr-1175 and Tyr-1214.
    • Cellular localization

      Membrane.
    • Target information above from: UniProt accession P35968 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 25589 Rat
      • SwissProt: O08775 Rat
      • Unigene: 88869 Rat
      • Alternative names

        • CD309 antibody
        • CD309 antigen antibody
        • EC 2.7.10.1 antibody
        • Fetal liver kinase 1 antibody
        • FLK-1 antibody
        • FLK1 antibody
        • FLK1, mouse, homolog of antibody
        • Kdr antibody
        • Kinase insert domain receptor (a type III receptor tyrosine kinase) antibody
        • Kinase insert domain receptor antibody
        • KRD1 antibody
        • Ly73 antibody
        • Protein tyrosine kinase receptor FLK1 antibody
        • Protein-tyrosine kinase receptor flk-1 antibody
        • soluble VEGFR2 antibody
        • Tyrosine kinase growth factor receptor antibody
        • Vascular endothelial growth factor receptor 2 antibody
        • VEGFR 2 antibody
        • VEGFR antibody
        • VEGFR-2 antibody
        • VEGFR2 antibody
        • VGFR2_HUMAN antibody
        see all

      Images

      • Western blot - Anti-VEGF Receptor 2 (phospho Y1214) antibody (ab5475)
        Western blot - Anti-VEGF Receptor 2 (phospho Y1214) antibody (ab5475)
        Peptide Competition and Stimulation: Extracts prepared from CSF-1 stimulated (1-4, 6) or unstimulated (5) PAE cells transfected with a chimeric CSF-1/VEGFR2 receptor were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4°C, then were incubated with 0.50 µg/mL ab5475 antibody for two hours at room temperature in a 3% BSA-TBST buffer, following prior incubation with: no peptide (1, 5, 6), the nonphosphopeptide corresponding to the immunogen (2), a generic phosphotyrosine containing peptide (3), or, the phosphopeptide immunogen (4). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG alkaline phosphatase and bands were detected using the Tropix WesternStar method. The data show that only the peptide corresponding to ab5475 blocks the antibody signal, and the stimulation of the phospho signal after stimulating ligand is added (CSF-1 for the chimeric r

      Protocols

      • Peptide Competition Experiment
      • Western blotting protocol

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (9)

      Publishing research using ab5475? Please let us know so that we can cite the reference in this datasheet.

      ab5475 has been referenced in 9 publications.

      • Chen X  et al. P53-induced miR-1249 inhibits tumor growth, metastasis, and angiogenesis by targeting VEGFA and HMGA2. Cell Death Dis 10:131 (2019). PubMed: 30755600
      • Yang GL  et al. TNFSF15 inhibits VEGF-stimulated vascular hyperpermeability by inducing VEGFR2 dephosphorylation. FASEB J 31:2001-2012 (2017). PubMed: 28183800
      • Arya P  et al. The endocytic recycling regulatory protein EHD1 Is required for ocular lens development. Dev Biol 408:41-55 (2015). PubMed: 26455409
      • Hung YW  et al. Monocyte chemoattractant protein-1 affects migration of hippocampal neural progenitors following status epilepticus in rats. J Neuroinflammation 10:11 (2013). IHC . PubMed: 23339567
      • Liu Z  et al. In vivo Notch reactivation in differentiating cochlear hair cells induces Sox2 and Prox1 expression but does not disrupt hair cell maturation. Dev Dyn 241:684-96 (2012). PubMed: 22354878
      • Färkkilä A  et al. Serum vascular endothelial growth factor A (VEGF) is elevated in patients with ovarian granulosa cell tumor (GCT), and VEGF inhibition by bevacizumab induces apoptosis in GCT in vitro. J Clin Endocrinol Metab 96:E1973-81 (2011). WB, IHC ; Human . PubMed: 21994955
      • Burgess D  et al. Activated Ras alters lens and corneal development through induction of distinct downstream targets. BMC Dev Biol 10:13 (2010). IHC . PubMed: 20105280
      • Yeh J  et al. Ovarian vascular endothelial growth factor and vascular endothelial growth factor receptor patterns in reproductive aging. Fertil Steril 89:1546-56 (2008). PubMed: 17889862
      • Wu L  et al. A simple method for obtaining transferrins from human plasma and porcine serum: preparations and properties. J Chromatogr B Analyt Technol Biomed Life Sci 867:62-8 (2008). PubMed: 18396113

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      Question

      I have a question about Flk-1 antibody. Does Flk-1 expressed in all endothelial cells or just active endothelial cells, like with agiogenesis. I did a stain for mouse heart by use, CD31 ( pharmingen), Von factor VIII( abcam), and Flk-1, Both CD31 and VIII are positive in heart vessels, but Flk-1 is negative, I don't know if it is because of antibody problem, what if I use ab5475? Thanks, Sincerely,

      Read More

      Abcam community

      Verified customer

      Asked on Jan 13 2004

      Answer

      Thank you for your enquiry. This antibody will recognize VEGFR-2 only if it is phosphorylated on Y1214.We would like to emphasize the fact that this antibody has only been tested for Western blot application.

      Read More

      Abcam Scientific Support

      Answered on Jan 15 2004

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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