Anti-VEGF Receptor 2 (phospho Y1214) antibody (ab5475)
Key features and details
- Rabbit polyclonal to VEGF Receptor 2 (phospho Y1214)
- Suitable for: WB
- Reacts with: Human, Recombinant fragment
- Isotype: IgG
Overview
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Product name
Anti-VEGF Receptor 2 (phospho Y1214) antibody
See all VEGF Receptor 2 primary antibodies -
Description
Rabbit polyclonal to VEGF Receptor 2 (phospho Y1214) -
Host species
Rabbit -
Specificity
VEGFR3 has not been tested, but is expected to react. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human, Recombinant fragment
Predicted to work with: Rat
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Immunogen
Synthetic peptide corresponding to Human VEGF Receptor 2 (phospho Y1214).
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Positive control
- WB: NIH 3T3 cells transfected with full length, wild-type human VEGFR 2 and Porcine Aortic Endothelial cells transfected with a chimeric receptor consisting of the extracellular domain of the CSF 1 receptor coupled to the transmembrane and cytoplasmic domains of the mouse VEGFR 2. HT-29 Serum Starved followed by treatment for 10 minutes with 100 ng/mL of SCF, Caco-2 Serum Starved followed by treatment for 10 minutes with 100 ng/mL of SCF.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.3
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.1% BSA
BSA is IgG and protease free -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated VEGFR 2 protein. The final product is generated by affinity chromatography using a VEGFR 2 derived peptide that is phosphorylated at Tyrosine 1214. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab5475 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use a concentration of 0.35 - 1 µg/ml. Detects a band of approximately 200 kDa.
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| Notes |
|---|
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WB
Use a concentration of 0.35 - 1 µg/ml. Detects a band of approximately 200 kDa. |
Target
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Function
Receptor for VEGF or VEGFC. Has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. -
Involvement in disease
Defects in KDR are associated with susceptibility to hemangioma capillary infantile (HCI) [MIM:602089]. HCI are benign, highly proliferative lesions involving aberrant localized growth of capillary endothelium. They are the most common tumor of infancy, occurring in up to 10% of all births. Hemangiomas tend to appear shortly after birth and show rapid neonatal growth for up to 12 months characterized by endothelial hypercellularity and increased numbers of mast cells. This phase is followed by slow involution at a rate of about 10% per year and replacement by fibrofatty stroma. -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
Contains 7 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain. -
Post-translational
modificationsPhosphorylated. Dephosphorylated by PTPRB. Dephosphorylated by PTPRJ at Tyr-951, Tyr-996, Tyr-1054, Tyr-1059, Tyr-1175 and Tyr-1214. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 3791 Human
- Entrez Gene: 25589 Rat
- Omim: 191306 Human
- SwissProt: P35968 Human
- SwissProt: O08775 Rat
- Unigene: 479756 Human
- Unigene: 88869 Rat
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Alternative names
- CD309 antibody
- CD309 antigen antibody
- EC 2.7.10.1 antibody
see all
Images
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Western blot - Anti-VEGF Receptor 2 (phospho Y1214) antibody (ab5475)Peptide Competition and Stimulation: Extracts prepared from CSF-1 stimulated (1-4, 6) or unstimulated (5) PAE cells transfected with a chimeric CSF-1/VEGFR2 receptor were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4°C, then were incubated with 0.50
µ g/mL ab5475 antibody for two hours at room temperature in a 3% BSA-TBST buffer, following prior incubation with: no peptide (1, 5, 6), the nonphosphopeptide corresponding to the immunogen (2), a generic phosphotyrosine containing peptide (3), or, the phosphopeptide immunogen (4). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG alkaline phosphatase and bands were detected using the Tropix WesternStar method. The data show that only the peptide corresponding to ab5475 blocks the antibody signal, and the stimulation of the phospho signal after stimulating ligand is added (CSF-1 for the chimeric r -
Western blot - Anti-VEGF Receptor 2 (phospho Y1214) antibody (ab5475)All lanes : Anti-VEGF Receptor 2 (phospho Y1214) antibody (ab5475) at 1/1000 dilution
Lane 1 : HT-29 (Human colorectal adenocarcinoma cell line) whole cell lysate
Lane 2 : HT-29 Serum Starved followed by treatment for 10 minutes with 100 ng/mL of SCF
Lane 3 : Caco-2 (Human colorectal adenocarcinoma cell line) whole cell lysate
Lane 4 : Caco-2 Serum Starved followed by treatment for 10 minutes with 100 ng/mL of SCF
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate at 1/5000 dilution
Observed band size: ~151 kDa why is the actual band size different from the predicted?Modulation of expression of target protein by cell treatment to demonstrate antibody specificity.
The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate
Protocols
Datasheets and documents
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Datasheet download
References (10)
ab5475 has been referenced in 10 publications.
- Hu L et al. Loss of the RNA-binding protein Rbm15 disrupts liver maturation in zebrafish. J Biol Chem 295:11466-11472 (2020). PubMed: 32518161
- Chen X et al. P53-induced miR-1249 inhibits tumor growth, metastasis, and angiogenesis by targeting VEGFA and HMGA2. Cell Death Dis 10:131 (2019). PubMed: 30755600
- Yang GL et al. TNFSF15 inhibits VEGF-stimulated vascular hyperpermeability by inducing VEGFR2 dephosphorylation. FASEB J 31:2001-2012 (2017). PubMed: 28183800
- Arya P et al. The endocytic recycling regulatory protein EHD1 Is required for ocular lens development. Dev Biol 408:41-55 (2015). PubMed: 26455409
- Hung YW et al. Monocyte chemoattractant protein-1 affects migration of hippocampal neural progenitors following status epilepticus in rats. J Neuroinflammation 10:11 (2013). IHC . PubMed: 23339567
- Liu Z et al. In vivo Notch reactivation in differentiating cochlear hair cells induces Sox2 and Prox1 expression but does not disrupt hair cell maturation. Dev Dyn 241:684-96 (2012). PubMed: 22354878
- Färkkilä A et al. Serum vascular endothelial growth factor A (VEGF) is elevated in patients with ovarian granulosa cell tumor (GCT), and VEGF inhibition by bevacizumab induces apoptosis in GCT in vitro. J Clin Endocrinol Metab 96:E1973-81 (2011). WB, IHC ; Human . PubMed: 21994955
- Burgess D et al. Activated Ras alters lens and corneal development through induction of distinct downstream targets. BMC Dev Biol 10:13 (2010). IHC . PubMed: 20105280
- Yeh J et al. Ovarian vascular endothelial growth factor and vascular endothelial growth factor receptor patterns in reproductive aging. Fertil Steril 89:1546-56 (2008). PubMed: 17889862
- Wu L et al. A simple method for obtaining transferrins from human plasma and porcine serum: preparations and properties. J Chromatogr B Analyt Technol Biomed Life Sci 867:62-8 (2008). PubMed: 18396113
