Anti-VEGF Receptor 3 antibody [44CT92.4.4] (ab72240)

Overview

  • Product name
    Anti-VEGF Receptor 3 antibody [44CT92.4.4]
    See all VEGF Receptor 3 primary antibodies
  • Description
    Mouse monoclonal [44CT92.4.4] to VEGF Receptor 3
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human VEGF Receptor 3.

  • Positive control
    • VGFR3 recombinant fragment and human lung carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab72240 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Detects a band of approximately 50 kDa (predicted molecular weight: 146 kDa).
ELISA 1/1000.
IHC-P 1/10 - 1/50.

Target

  • Function
    Receptor for VEGFC. Has a tyrosine-protein kinase activity.
  • Tissue specificity
    Placenta, lung, heart, and kidney, does not seem to be expressed in pancreas and brain.
  • Involvement in disease
    Defects in FLT4 are the cause of lymphedema hereditary type 1A (LMPH1A) [MIM:153100]; also known as Nonne-Milroy lymphedema or Milroy disease. Hereditary lymphedema is a chronic disabling condition which results in swelling of the extremities due to altered lymphatic flow. Patients with lymphedema suffer from recurrent local infections and physical impairment.
    Note=Defects in FLT4 are found in juvenile hemangioma. Juvenile hemangiomas are the most common tumors of infancy, occurring as many as 10% of all births. These benign vascular lesions enlarge rapidly during the first year of life by hyperplasia of endothelial cells and attendant pericytes, and then spontaneously involute over a period of years, leaving loose fibrofatty tissue.
  • Sequence similarities
    Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
    Contains 7 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • EC 2.7.10.1 antibody
    • flt 4 antibody
    • FLT-4 antibody
    • FLT4 antibody
    • FLT41 antibody
    • Fms related tyrosine kinase 4 antibody
    • Fms-like tyrosine kinase 4 antibody
    • LMPH1A antibody
    • PCL antibody
    • Soluble VEGFR3 variant 1 antibody
    • Soluble VEGFR3 variant 2 antibody
    • Soluble VEGFR3 variant 3 antibody
    • Tyrosine protein kinase receptor FLT4 antibody
    • Tyrosine-protein kinase receptor FLT4 antibody
    • Vascular endothelial growth factor receptor 3 antibody
    • Vascular endothelial growth factor receptor 3 precursor antibody
    • VEGF R3 antibody
    • VEGFR 3 antibody
    • VEGFR-3 antibody
    • VEGFR3 antibody
    • VGFR3_HUMAN antibody
    see all

Images

  • Anti-VEGF Receptor 3 antibody [44CT92.4.4] (ab72240) at 1/2000 dilution + VGFR3 recombinant fragment

    Predicted band size: 146 kDa
    Observed band size: 50 kDa (why is the actual band size different from the predicted?)



    The molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the protein was used.
  • ab72240 at 1/10 dilution, staining VEGF Receptor 3 in human lung carcinoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue, followed by peroxidase-conjugated secondary antibody and DAB staining.

References

This product has been referenced in:
  • Kim HJ  et al. Association Between c-Met and Lymphangiogenic Factors in Patients With Colorectal Cancer. Ann Coloproctol 34:88-93 (2018). Read more (PubMed: 29742856) »
  • Singh N  et al. Soluble vascular endothelial growth factor receptor 3 is essential for corneal alymphaticity. Blood 121:4242-9 (2013). Mouse . Read more (PubMed: 23476047) »

See all 2 Publications for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
H2O2 as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: 25°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate, pH6, 100C, 20min
Sample
Human Tissue sections (angiosarcoma)
Specification
angiosarcoma
Permeabilization
No
Fixative
Formaldehyde
Username

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Submitted Sep 12 2014

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