Anti-VEGF Receptor 3 antibody (ab10284)
- Datasheet
- References (1)
- Protocols
Overview
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Product nameAnti-VEGF Receptor 3 antibody
See all VEGF Receptor 3 primary antibodies -
DescriptionRabbit polyclonal to VEGF Receptor 3
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Host speciesRabbit
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SpecificityIn Western blots, this antibody shows a moderate cross-reactivity with VEGFR2/KDR and a weak cross-reactivity with VEGFR1/FLT1.
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Tested applicationsSuitable for: Flow Cyt, IHC-Fr, ICC/IF, ELISA, WB, IPmore details
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Species reactivityReacts with: Human
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Immunogen
Recombinant fragment corresponding to Human VEGF Receptor 3. Recombinant human soluble VEGFR3/FLT4 protein (110 kDa) (D1-D7).
Database link: P35916
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.40
Constituent: PBS -
Concentration information loading... -
PurityProtein A purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab10284 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
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| Flow Cyt | Use a concentration of 1 - 5 µg/ml. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
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| IHC-Fr | Use at an assay dependent concentration. | |
| ICC/IF | Use at an assay dependent concentration. | |
| ELISA | Use a concentration of 1 - 5 µg/ml. | |
| WB | Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 164 kDa. | |
| IP | Use a concentration of 1 - 5 µg/ml. PubMed: 19366703 |
Target
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FunctionReceptor for VEGFC. Has a tyrosine-protein kinase activity.
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Tissue specificityPlacenta, lung, heart, and kidney, does not seem to be expressed in pancreas and brain.
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Involvement in diseaseDefects in FLT4 are the cause of lymphedema hereditary type 1A (LMPH1A) [MIM:153100]; also known as Nonne-Milroy lymphedema or Milroy disease. Hereditary lymphedema is a chronic disabling condition which results in swelling of the extremities due to altered lymphatic flow. Patients with lymphedema suffer from recurrent local infections and physical impairment.
Note=Defects in FLT4 are found in juvenile hemangioma. Juvenile hemangiomas are the most common tumors of infancy, occurring as many as 10% of all births. These benign vascular lesions enlarge rapidly during the first year of life by hyperplasia of endothelial cells and attendant pericytes, and then spontaneously involute over a period of years, leaving loose fibrofatty tissue. -
Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
Contains 7 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain. -
Cellular localizationMembrane.
- Information by UniProt
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Database links
- Entrez Gene: 2324 Human
- Omim: 136352 Human
- SwissProt: P35916 Human
- Unigene: 646917 Human
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Alternative names
- EC 2.7.10.1 antibody
- flt 4 antibody
- FLT-4 antibody
see all
Images
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Flow Cytometry - Anti-VEGF Receptor 3 antibody (ab10284)Flow Cytometry analysis of human dermal lymphatic endothelial cells (HDLEC) labelling VEGF Receptor 3 with ab10284.
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Immunohistochemistry (Frozen sections) - Anti-VEGF Receptor 3 antibody (ab10284)Immunohistochemistry (Frozen sections) analysis of human spleen tissue labelling VEGF Receptor 3 with ab10284.
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Immunocytochemistry/ Immunofluorescence - Anti-VEGF Receptor 3 antibody (ab10284)Immunocytochemistry/Immunofluorescence analysis of human VEGF Receptor 3 transfected MG63 cells labelling VEGF Receptor 3 (red) with ab10284 (D). A and C are negative controls with secondary antibody only. B uses a monoclonal antibody specific for VEGF Receptor 3.
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Immunoprecipitation - Anti-VEGF Receptor 3 antibody (ab10284)Image from Toivanen PI et al, J Biol Chem. 2009 Jun 5;284(23):16037-48. Epub 2009 Apr 14, Fig 7. DOI 10.1074/jbc.M109.001123Serum-starved porcine aortic endothelial cells were incubated with recombinant VEGF proteins, and the phosphorylation status of VEGFRs or Akt was analyzed from cell lysates. The effect of VEGF-D?N?C and C25L on the phosphorylation of VEGFR-3 in PAE-Flt4 cells at 5, 15, and 30 minute time points. Cell lysates were immunoprecipitated using ab10284 and analyzed by immunoblotting with an anti phosphotyrosine antibody. Equal loading was confirmed using ab10284.
Protocols
Datasheets and documents
References
This product has been referenced in:
- Toivanen PI et al. Novel vascular endothelial growth factor D variants with increased biological activity. J Biol Chem 284:16037-48 (2009). IP ; Pig . Read more (PubMed: 19366703) »
