Anti-VEGF Receptor 3 antibody (ab10284)
Key features and details
- Rabbit polyclonal to VEGF Receptor 3
- Suitable for: Flow Cyt, IHC-Fr, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-VEGF Receptor 3 antibody
See all VEGF Receptor 3 primary antibodies -
Description
Rabbit polyclonal to VEGF Receptor 3 -
Host species
Rabbit -
Specificity
In Western blots, this antibody shows a moderate cross-reactivity with VEGFR2/KDR and a weak cross-reactivity with VEGFR1/FLT1. -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanIHC-Fr Human
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Immunogen
Recombinant fragment corresponding to Human VEGF Receptor 3. Recombinant human soluble VEGFR3/FLT4 protein (110 kDa) (D1-D7).
Database link: P35916
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab10284 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| Flow Cyt |
Human
|
| ICC/IF |
Human
|
| IHC-Fr |
Human
|
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
Use a concentration of 1 - 5 µg/ml.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| IHC-Fr |
Use at an assay dependent concentration.
|
|
| ICC/IF |
Use at an assay dependent concentration.
|
| Notes |
|---|
|
Flow Cyt
Use a concentration of 1 - 5 µg/ml. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
|
IHC-Fr
Use at an assay dependent concentration. |
|
ICC/IF
Use at an assay dependent concentration. |
Target
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Function
Receptor for VEGFC. Has a tyrosine-protein kinase activity. -
Tissue specificity
Placenta, lung, heart, and kidney, does not seem to be expressed in pancreas and brain. -
Involvement in disease
Defects in FLT4 are the cause of lymphedema hereditary type 1A (LMPH1A) [MIM:153100]; also known as Nonne-Milroy lymphedema or Milroy disease. Hereditary lymphedema is a chronic disabling condition which results in swelling of the extremities due to altered lymphatic flow. Patients with lymphedema suffer from recurrent local infections and physical impairment.
Note=Defects in FLT4 are found in juvenile hemangioma. Juvenile hemangiomas are the most common tumors of infancy, occurring as many as 10% of all births. These benign vascular lesions enlarge rapidly during the first year of life by hyperplasia of endothelial cells and attendant pericytes, and then spontaneously involute over a period of years, leaving loose fibrofatty tissue. -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
Contains 7 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 2324 Human
- Omim: 136352 Human
- SwissProt: P35916 Human
- Unigene: 646917 Human
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Alternative names
- EC 2.7.10.1 antibody
- flt 4 antibody
- FLT-4 antibody
see all
Images
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Flow Cytometry - Anti-VEGF Receptor 3 antibody (ab10284)
Flow Cytometry analysis of human dermal lymphatic endothelial cells (HDLEC) labelling VEGF Receptor 3 with ab10284.
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Immunohistochemistry (Frozen sections) - Anti-VEGF Receptor 3 antibody (ab10284)Immunohistochemistry (Frozen sections) analysis of human spleen tissue labelling VEGF Receptor 3 with ab10284.
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Immunocytochemistry/ Immunofluorescence - Anti-VEGF Receptor 3 antibody (ab10284)Immunocytochemistry/Immunofluorescence analysis of human VEGF Receptor 3 transfected MG63 cells labelling VEGF Receptor 3 (red) with ab10284 (D). A and C are negative controls with secondary antibody only. B uses a monoclonal antibody specific for VEGF Receptor 3.
Protocols
Datasheets and documents
References (2)
ab10284 has been referenced in 2 publications.
- Yu DS et al. Chaperones, Membrane Trafficking and Signal Transduction Proteins Regulate Zaire Ebola Virus trVLPs and Interact With trVLP Elements. Front Microbiol 9:2724 (2018). PubMed: 30483236
- Toivanen PI et al. Novel vascular endothelial growth factor D variants with increased biological activity. J Biol Chem 284:16037-48 (2009). IP ; Pig . PubMed: 19366703
