Overview

  • Product name
    Anti-VEGF Receptor 3 antibody
    See all VEGF Receptor 3 primary antibodies
  • Description
    Rabbit polyclonal to VEGF Receptor 3
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC - Wholemount, ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human VEGF Receptor 3 aa 1250-1350. The exact sequence is proprietary.
    Database link: P35916

  • Positive control
    • Human placenta or breast carcinoma for Immunohistochemistry.

Properties

Applications

Our Abpromise guarantee covers the use of ab27278 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC - Wholemount 1/200.
ICC/IF Use a concentration of 1 µg/ml.
WB Use at an assay dependent concentration. Predicted molecular weight: 146 kDa.
IHC-P 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Function
    Receptor for VEGFC. Has a tyrosine-protein kinase activity.
  • Tissue specificity
    Placenta, lung, heart, and kidney, does not seem to be expressed in pancreas and brain.
  • Involvement in disease
    Defects in FLT4 are the cause of lymphedema hereditary type 1A (LMPH1A) [MIM:153100]; also known as Nonne-Milroy lymphedema or Milroy disease. Hereditary lymphedema is a chronic disabling condition which results in swelling of the extremities due to altered lymphatic flow. Patients with lymphedema suffer from recurrent local infections and physical impairment.
    Note=Defects in FLT4 are found in juvenile hemangioma. Juvenile hemangiomas are the most common tumors of infancy, occurring as many as 10% of all births. These benign vascular lesions enlarge rapidly during the first year of life by hyperplasia of endothelial cells and attendant pericytes, and then spontaneously involute over a period of years, leaving loose fibrofatty tissue.
  • Sequence similarities
    Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
    Contains 7 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • EC 2.7.10.1 antibody
    • flt 4 antibody
    • FLT-4 antibody
    • FLT4 antibody
    • FLT41 antibody
    • Fms related tyrosine kinase 4 antibody
    • Fms-like tyrosine kinase 4 antibody
    • LMPH1A antibody
    • PCL antibody
    • Soluble VEGFR3 variant 1 antibody
    • Soluble VEGFR3 variant 2 antibody
    • Soluble VEGFR3 variant 3 antibody
    • Tyrosine protein kinase receptor FLT4 antibody
    • Tyrosine-protein kinase receptor FLT4 antibody
    • Vascular endothelial growth factor receptor 3 antibody
    • Vascular endothelial growth factor receptor 3 precursor antibody
    • VEGF R3 antibody
    • VEGFR 3 antibody
    • VEGFR-3 antibody
    • VEGFR3 antibody
    • VGFR3_HUMAN antibody
    see all

Images

  • Ab27278 at a dilution of 1/100, staining formalin fixed paraffin embedded Flt4 in human placenta by Immunohistochemistry.
  • ICC/IF image of ab27278 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab27278, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-VEGF Receptor 3 antibody (ab27278) at 1/50 dilution
    Predicted band size: 146 kDa
    Observed band size: 170,200 kDa
    why is the actual band size different from the predicted?



    Hey cell lysate (50µg/lane) was used. The antibody was diluted at 1:50 for 2 hours at room tepterature Hey cell lysate (50µg/lane) was used. The antibody was diluted at 1:50 for 2 hours at room temperature. The protein is heavily glycosylated and this might explain the increase in MW above the predcted MW as well as the multiple bands.

References

This product has been referenced in:
  • Zhou J  et al. MiR-128-3p directly targets VEGFC/VEGFR3 to modulate the proliferation of lymphatic endothelial cells through Ca2+ signaling. Int J Biochem Cell Biol N/A:N/A (2018). WB ; Human . Read more (PubMed: 29777777) »
  • Milasan A  et al. Apolipoprotein A-I Modulates Atherosclerosis Through Lymphatic Vessel-Dependent Mechanisms in Mice. J Am Heart Assoc 6:N/A (2017). Read more (PubMed: 28939717) »
See all 16 Publications for this product

Customer reviews and Q&As

1-10 of 12 Abreviews or Q&A

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (small intestine)
Permeabilization
Yes - 0.25% PBST (Triton X 100)
Specification
small intestine
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 2.5% · Temperature: RT°C
Fixative
Paraformaldehyde

Dr. Kento Tazawa

Verified customer

Submitted Apr 14 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (PLACENTA)
Antigen retrieval step
Enzymatic
Permeabilization
No
Specification
PLACENTA
Blocking step
Serum as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Jun 22 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Skin)
Specification
Skin
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer pH 6.0
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Dec 18 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
IHC - Wholemount
Sample
Rat Tissue (brain)
Specification
brain

Xiao Mao

Verified customer

Submitted Dec 17 2012

Question
Answer

I am very pleased to hear you would like to accept our offer and test ab27278 in rat. This code will give you the VALUE of this product off an order before the expiration date. To redeem this offer, please submit an Abreview for rat. The code is inactive until included in your submitted Abreview so be sure to add it to the Additional Comments section. For more information on how to submit an Abreview, please visit the site: https://www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: https://www.abcam.com/AbTrial

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Question
Answer

Thank you for contacting us. I have created a new 100% Abreview Testing Discount Code for testing AB27278 in Porcine. This code will expire on xxxxxxx. xxxxx I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information. Use our products? Submit an Abreview. Earn rewards! https://www.abcam.com/abreviews

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Answer

Thank you very much for your interest in 27278.

To our knowledge, ab27278 has not been tested in IHC-Fr. Therefore, I can offer a discount off a future purchase if you buy ab27278 now, test it in IHC-Fr and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody.

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test ab27278 in IHC-Fr. I will then send a discount code. This code must be issued before purchasing ab27278 so please wait for my reply before ordering.

2. Purchase ab27278 either by phone, fax, or online (https://www.abcam.com).

3. Test it in IHC-Fr.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue.
Please remember that submission of the Abreview is sufficient for the discount code to become active.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab27278 turns out to be unsuitable for IHC-Fr, you will still receive the discount on your next purchase after your Abreview has been submitted.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: https://www.abcam.com/collaborationdiscount.

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Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Cow Tissue sections (Ovary)
Specification
Ovary
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA
Permeabilization
No
Blocking step
Serum as blocking agent for 40 minute(s) · Concentration: 2.5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Jul 31 2012

Application
Western blot
Sample
Pig Tissue lysate - whole (Pig heart (left ventricle))
Loading amount
50 µg
Specification
Pig heart (left ventricle)
Gel Running Conditions
Reduced Denaturing (3-8% Novex Tris-Acetate)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Apr 09 2012

Answer

I am very pleased to hear you would like to accept our offer and test ab27278 in porcine tissues. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for porcine tissues and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

Read More

1-10 of 12 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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