Overview

  • Product name
    Anti-VEGF Receptor 3 antibody
    See all VEGF Receptor 3 primary antibodies
  • Description
    Rabbit polyclonal to VEGF Receptor 3
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC - Wholemount, ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human VEGF Receptor 3 aa 1250-1350. The exact sequence is proprietary.
    Database link: P35916

  • Positive control
    • Human placenta or breast carcinoma for Immunohistochemistry.

Properties

Applications

Our Abpromise guarantee covers the use of ab27278 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC - Wholemount 1/200.
ICC/IF Use a concentration of 1 µg/ml.
WB Use at an assay dependent concentration. Predicted molecular weight: 146 kDa.
IHC-P 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Function
    Receptor for VEGFC. Has a tyrosine-protein kinase activity.
  • Tissue specificity
    Placenta, lung, heart, and kidney, does not seem to be expressed in pancreas and brain.
  • Involvement in disease
    Defects in FLT4 are the cause of lymphedema hereditary type 1A (LMPH1A) [MIM:153100]; also known as Nonne-Milroy lymphedema or Milroy disease. Hereditary lymphedema is a chronic disabling condition which results in swelling of the extremities due to altered lymphatic flow. Patients with lymphedema suffer from recurrent local infections and physical impairment.
    Note=Defects in FLT4 are found in juvenile hemangioma. Juvenile hemangiomas are the most common tumors of infancy, occurring as many as 10% of all births. These benign vascular lesions enlarge rapidly during the first year of life by hyperplasia of endothelial cells and attendant pericytes, and then spontaneously involute over a period of years, leaving loose fibrofatty tissue.
  • Sequence similarities
    Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
    Contains 7 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • EC 2.7.10.1 antibody
    • flt 4 antibody
    • FLT-4 antibody
    • FLT4 antibody
    • FLT41 antibody
    • Fms related tyrosine kinase 4 antibody
    • Fms-like tyrosine kinase 4 antibody
    • LMPH1A antibody
    • PCL antibody
    • Soluble VEGFR3 variant 1 antibody
    • Soluble VEGFR3 variant 2 antibody
    • Soluble VEGFR3 variant 3 antibody
    • Tyrosine protein kinase receptor FLT4 antibody
    • Tyrosine-protein kinase receptor FLT4 antibody
    • Vascular endothelial growth factor receptor 3 antibody
    • Vascular endothelial growth factor receptor 3 precursor antibody
    • VEGF R3 antibody
    • VEGFR 3 antibody
    • VEGFR-3 antibody
    • VEGFR3 antibody
    • VGFR3_HUMAN antibody
    see all

Images

  • Ab27278 at a dilution of 1/100, staining formalin fixed paraffin embedded Flt4 in human placenta by Immunohistochemistry.
  • ICC/IF image of ab27278 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab27278, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-VEGF Receptor 3 antibody (ab27278) at 1/50 dilution
    Predicted band size: 146 kDa
    Observed band size: 170,200 kDa
    why is the actual band size different from the predicted?



    Hey cell lysate (50µg/lane) was used. The antibody was diluted at 1:50 for 2 hours at room tepterature Hey cell lysate (50µg/lane) was used. The antibody was diluted at 1:50 for 2 hours at room temperature. The protein is heavily glycosylated and this might explain the increase in MW above the predcted MW as well as the multiple bands.

References

This product has been referenced in:
  • Zhou J  et al. MiR-128-3p directly targets VEGFC/VEGFR3 to modulate the proliferation of lymphatic endothelial cells through Ca2+ signaling. Int J Biochem Cell Biol N/A:N/A (2018). WB ; Human . Read more (PubMed: 29777777) »
  • Hong B  et al. Activation of the LKB1-SIK1 signaling pathway inhibits the TGF-ß-mediated epithelial-mesenchymal transition and apoptosis resistance of ovarian carcinoma cells. Mol Med Rep 17:2837-2844 (2018). Read more (PubMed: 29257268) »
See all 18 Publications for this product

Customer reviews and Q&As

Filter by Application

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1-6 of 6 Abreviews

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (small intestine)
Permeabilization
Yes - 0.25% PBST (Triton X 100)
Specification
small intestine
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 2.5% · Temperature: RT°C
Fixative
Paraformaldehyde

Dr. Kento Tazawa

Verified customer

Submitted Apr 14 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (PLACENTA)
Antigen retrieval step
Enzymatic
Permeabilization
No
Specification
PLACENTA
Blocking step
Serum as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Jun 22 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Skin)
Specification
Skin
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer pH 6.0
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Dec 18 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
IHC - Wholemount
Sample
Rat Tissue (brain)
Specification
brain

Xiao Mao

Verified customer

Submitted Dec 17 2012

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Cow Tissue sections (Ovary)
Specification
Ovary
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA
Permeabilization
No
Blocking step
Serum as blocking agent for 40 minute(s) · Concentration: 2.5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Jul 31 2012

Application
Western blot
Sample
Pig Tissue lysate - whole (Pig heart (left ventricle))
Loading amount
50 µg
Specification
Pig heart (left ventricle)
Gel Running Conditions
Reduced Denaturing (3-8% Novex Tris-Acetate)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Apr 09 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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