Product nameAnti-VEGF Receptor 3 antibody [EPR22293-14]
See all VEGF Receptor 3 primary antibodies
DescriptionRabbit monoclonal [EPR22293-14] to VEGF Receptor 3
Tested applicationsSuitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
Species reactivityReacts with: Human
- WB: HEL whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab243232 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 175, 125, 80 kDa (predicted molecular weight: 146 kDa).|
FunctionReceptor for VEGFC. Has a tyrosine-protein kinase activity.
Tissue specificityPlacenta, lung, heart, and kidney, does not seem to be expressed in pancreas and brain.
Involvement in diseaseDefects in FLT4 are the cause of lymphedema hereditary type 1A (LMPH1A) [MIM:153100]; also known as Nonne-Milroy lymphedema or Milroy disease. Hereditary lymphedema is a chronic disabling condition which results in swelling of the extremities due to altered lymphatic flow. Patients with lymphedema suffer from recurrent local infections and physical impairment.
Note=Defects in FLT4 are found in juvenile hemangioma. Juvenile hemangiomas are the most common tumors of infancy, occurring as many as 10% of all births. These benign vascular lesions enlarge rapidly during the first year of life by hyperplasia of endothelial cells and attendant pericytes, and then spontaneously involute over a period of years, leaving loose fibrofatty tissue.
Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
Contains 7 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain.
- Information by UniProt
- EC 220.127.116.11 antibody
- flt 4 antibody
- FLT-4 antibody
All lanes : Anti-VEGF Receptor 3 antibody [EPR22293-14] (ab243232) at 1/1000 dilution
Lane 1 : HEL (human erythroleukemia erythroblast cell line) whole cell lysate
Lane 2 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 146 kDa
Observed band size: 125,175,80 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
This blot was developed using a higher sensitivity ECL substrate.
The molecular weight observed is consistent with the literatures (PMID: 18538738, PMID: 7692369, PMID: 14648657).
Negative control: MCF7 (PMID: 18538738).
ab243232 has not yet been referenced specifically in any publications.