Anti-VEGF Receptor 3 antibody [MM0003-7G63] (ab51496)
- Datasheet
- Specific References (1)
- Protocols
Overview
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Product nameAnti-VEGF Receptor 3 antibody [MM0003-7G63]
See all VEGF Receptor 3 primary antibodies -
DescriptionMouse monoclonal [MM0003-7G63] to VEGF Receptor 3
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Host speciesMouse
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SpecificityNo cross-reactivity is shown with VEGFR1 or VEGFR2.
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Tested applicationsSuitable for: Flow Cyt, IHC-P, WBmore details
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Species reactivityReacts with: Human
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Immunogen
N-terminal recombinant fragment (Human)
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Storage bufferPreservative: None
Constituents: PBS -
Concentration information loading... -
PurityProtein G purified
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ClonalityMonoclonal
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Clone numberMM0003-7G63
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IsotypeIgG1
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab51496 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
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| Flow Cyt | Use 1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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| IHC-P | Use at an assay dependent concentration. | |
| WB | 1/500 - 1/1000. Predicted molecular weight: 146 kDa. |
Target
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FunctionReceptor for VEGFC. Has a tyrosine-protein kinase activity.
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Tissue specificityPlacenta, lung, heart, and kidney, does not seem to be expressed in pancreas and brain.
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Involvement in diseaseDefects in FLT4 are the cause of lymphedema hereditary type 1A (LMPH1A) [MIM:153100]; also known as Nonne-Milroy lymphedema or Milroy disease. Hereditary lymphedema is a chronic disabling condition which results in swelling of the extremities due to altered lymphatic flow. Patients with lymphedema suffer from recurrent local infections and physical impairment.
Note=Defects in FLT4 are found in juvenile hemangioma. Juvenile hemangiomas are the most common tumors of infancy, occurring as many as 10% of all births. These benign vascular lesions enlarge rapidly during the first year of life by hyperplasia of endothelial cells and attendant pericytes, and then spontaneously involute over a period of years, leaving loose fibrofatty tissue. -
Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
Contains 7 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain. -
Cellular localizationMembrane.
- Information by UniProt
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Database links
- Entrez Gene: 2324 Human
- Omim: 136352 Human
- SwissProt: P35916 Human
- Unigene: 646917 Human
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Alternative names
- EC 2.7.10.1 antibody
- flt 4 antibody
- FLT-4 antibody
see all
Images
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGF Receptor 3 antibody [MM0003-7G63] (ab51496)](https://www.abcam.com/ps/products/51/ab51496/Images/VEGF-Receptor-3-Primary-antibodies-ab51496-1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGF Receptor 3 antibody [MM0003-7G63] (ab51496)ab51496 at 1/200 dilution staining VEGF Receptor 3 in human placenta tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections).
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![Flow Cytometry - Anti-VEGF Receptor 3 antibody [MM0003-7G63] (ab51496)](https://www.abcam.com/ps/products/51/ab51496/Images/VEGF-Receptor-3-Primary-antibodies-ab51496-2.jpg)
Flow Cytometry - Anti-VEGF Receptor 3 antibody [MM0003-7G63] (ab51496)Overlay histogram showing HeLa cells stained with ab51496 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51496, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
References
This product has been referenced in:
- Brar R et al. Breast Angiosarcoma: Case Series and Expression of Vascular Endothelial Growth Factor. Case Rep Oncol 2:242-250 (2009). IHC-P ; Human . Read more (PubMed: 20737044) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"