Anti-VEGF Receptor 3 antibody [MM0003-7G63] (ab51496)

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Key features and details

  • Mouse monoclonal [MM0003-7G63] to VEGF Receptor 3
  • Suitable for: Flow Cyt, IHC-P
  • Reacts with: Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-VEGF Receptor 3 antibody [MM0003-7G63]
    See all VEGF Receptor 3 primary antibodies

  • Description

    Mouse monoclonal [MM0003-7G63] to VEGF Receptor 3

  • Host species

    Mouse

  • Specificity

    No cross-reactivity is shown with VEGFR1 or VEGFR2.

  • Tested applications

    Suitable for: Flow Cyt, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    N-terminal recombinant fragment (Human)

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab51496 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-P Use at an assay dependent concentration.

Target

  • Function

    Receptor for VEGFC. Has a tyrosine-protein kinase activity.

  • Tissue specificity

    Placenta, lung, heart, and kidney, does not seem to be expressed in pancreas and brain.

  • Involvement in disease

    Defects in FLT4 are the cause of lymphedema hereditary type 1A (LMPH1A) [MIM:153100]; also known as Nonne-Milroy lymphedema or Milroy disease. Hereditary lymphedema is a chronic disabling condition which results in swelling of the extremities due to altered lymphatic flow. Patients with lymphedema suffer from recurrent local infections and physical impairment.
    Note=Defects in FLT4 are found in juvenile hemangioma. Juvenile hemangiomas are the most common tumors of infancy, occurring as many as 10% of all births. These benign vascular lesions enlarge rapidly during the first year of life by hyperplasia of endothelial cells and attendant pericytes, and then spontaneously involute over a period of years, leaving loose fibrofatty tissue.

  • Sequence similarities

    Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
    Contains 7 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.

  • Cellular localization

    Membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • EC 2.7.10.1 antibody
      • flt 4 antibody
      • FLT-4 antibody
      • FLT4 antibody
      • FLT41 antibody
      • Fms related tyrosine kinase 4 antibody
      • Fms-like tyrosine kinase 4 antibody
      • LMPH1A antibody
      • PCL antibody
      • Soluble VEGFR3 variant 1 antibody
      • Soluble VEGFR3 variant 2 antibody
      • Soluble VEGFR3 variant 3 antibody
      • Tyrosine protein kinase receptor FLT4 antibody
      • Tyrosine-protein kinase receptor FLT4 antibody
      • Vascular endothelial growth factor receptor 3 antibody
      • Vascular endothelial growth factor receptor 3 precursor antibody
      • VEGF R3 antibody
      • VEGFR 3 antibody
      • VEGFR-3 antibody
      • VEGFR3 antibody
      • VGFR3_HUMAN antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGF Receptor 3 antibody [MM0003-7G63] (ab51496)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGF Receptor 3 antibody [MM0003-7G63] (ab51496)

      ab51496 at 1/200 dilution staining VEGF Receptor 3 in human placenta tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections).

    • Flow Cytometry - Anti-VEGF Receptor 3 antibody [MM0003-7G63] (ab51496)
      Flow Cytometry - Anti-VEGF Receptor 3 antibody [MM0003-7G63] (ab51496)
      Overlay histogram showing HeLa cells stained with ab51496 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51496, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

      Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

    References (1)

    Publishing research using ab51496? Please let us know so that we can cite the reference in this datasheet.

    ab51496 has been referenced in 1 publication.

    • Brar R  et al. Breast Angiosarcoma: Case Series and Expression of Vascular Endothelial Growth Factor. Case Rep Oncol 2:242-250 (2009). IHC-P ; Human . PubMed: 20737044

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