Anti-VEGFA antibody [11B5] (ab38909)

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Overview

  • Product name
    Anti-VEGFA antibody [11B5]
    See all VEGFA primary antibodies
  • Description
    Mouse monoclonal [11B5] to VEGFA
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, IHC-Fr, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Guinea pig, Human
  • Immunogen

    Synthetic peptide corresponding to Human VEGFA (N terminal).

Applications

Our Abpromise guarantee covers the use of ab38909 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Predicted molecular weight: 24 kDa.
IHC-Fr 1/10.
IHC-P Use at an assay dependent dilution.

Target

  • Function
    Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth.
  • Tissue specificity
    Isoform VEGF189, isoform VEGF165 and isoform VEGF121 are widely expressed. Isoform VEGF206 and isoform VEGF145 are not widely expressed.
  • Involvement in disease
    Defects in VEGFA are a cause of susceptibility to microvascular complications of diabetes type 1 (MVCD1) [MIM:603933]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis.
  • Sequence similarities
    Belongs to the PDGF/VEGF growth factor family.
  • Cellular localization
    Secreted. VEGF121 is acidic and freely secreted. VEGF165 is more basic, has heparin-binding properties and, although a signicant proportion remains cell-associated, most is freely secreted. VEGF189 is very basic, it is cell-associated after secretion and is bound avidly by heparin and the extracellular matrix, although it may be released as a soluble form by heparin, heparinase or plasmin.
  • Information by UniProt
  • Database links
    • Alternative names
      • Folliculostellate cell-derived growth factor antibody
      • Glioma-derived endothelial cell mitogen antibody
      • MGC70609 antibody
      • MVCD1 antibody
      • Vascular endothelial growth factor A antibody
      • vascular endothelial growth factor A121 antibody
      • vascular endothelial growth factor A165 antibody
      • vascular endothelial growth factor antibody
      • Vascular permeability factor antibody
      • VEGF A antibody
      • Vegf antibody
      • VEGF-A antibody
      • VEGF120 antibody
      • Vegfa antibody
      • VEGFA_HUMAN antibody
      • VPF antibody
      see all

    References

    This product has been referenced in:
    • Giatromanolaki A  et al. Upregulated hypoxia inducible factor-1alpha and -2alpha pathway in rheumatoid arthritis and osteoarthritis. Arthritis Res Ther 5:R193-201 (2003). Read more (PubMed: 12823854) »
    See 1 Publication for this product

    Publishing research using ab38909? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    Question

    What is the stock concentration of the anti-VEGF antibody, ab38909?

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    Answer

    Thank you for contacting us.

    The lab let me know the following:

    Our antibody ab38909 was purified via Size Exclusion. The subclass for Clone # 11B5 is an IgM kappa. Since this is a cell culture supernatant, there will be a lot of non-specific protein from the growth medium too. So, we are cannot truly define the amount of the IgM in this product.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

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