Anti-VEGFA antibody [16F1] (ab155944)
![Western blot - Anti-VEGFA antibody [16F1] (ab155944)](https://www.abcam.com/ps/products/155/ab155944/Images/ab155944-163216-anti-vegfa-antibody-16f1-western-blot.jpg "Western blot - Anti-VEGFA antibody [16F1] (ab155944)")
Key features and details
- Mouse monoclonal [16F1] to VEGFA
- Suitable for: WB, Sandwich ELISA
- Reacts with: Human, Recombinant fragment
- Isotype: IgG2a
Overview
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Product name
Anti-VEGFA antibody [16F1]
See all VEGFA primary antibodies -
Description
Mouse monoclonal [16F1] to VEGFA -
Host species
Mouse -
Tested applications
Suitable for: WB, Sandwich ELISAmore details -
Species reactivity
Reacts with: Human, Recombinant fragment -
Immunogen
Recombinant full length Human VEGFA expressed in E-coli (P15692)
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Positive control
- Human VEGFA recombinant protein; Human VEGFA transfected 293T cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Constituent: 100% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
16F1 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab155944 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use a concentration of 1 µg/ml.
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| Sandwich ELISA |
Use a concentration of 1 µg/ml.
Typical dilutions for sandwich ELISA include 1µg/ml for coating and 0.125-0.5µg/ml for detection. Can be paired for Sandwich ELISA with Rabbit polyclonal to VEGFA (Biotin) (ab83132). To detect hVEGF by sandwich ELISA (using 100 µl/well antibody solution) a concentration of 0.5 - 2.0 µg/ml of this antibody as Detection is required. This antigen affinity purified antibody, paired with the above recommended pair, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hVEGF (ab9571). |
| Notes |
|---|
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WB
Use a concentration of 1 µg/ml. |
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Sandwich ELISA
Use a concentration of 1 µg/ml. Typical dilutions for sandwich ELISA include 1µg/ml for coating and 0.125-0.5µg/ml for detection. Can be paired for Sandwich ELISA with Rabbit polyclonal to VEGFA (Biotin) (ab83132). To detect hVEGF by sandwich ELISA (using 100 µl/well antibody solution) a concentration of 0.5 - 2.0 µg/ml of this antibody as Detection is required. This antigen affinity purified antibody, paired with the above recommended pair, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hVEGF (ab9571). |
Target
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Function
Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth. -
Tissue specificity
Isoform VEGF189, isoform VEGF165 and isoform VEGF121 are widely expressed. Isoform VEGF206 and isoform VEGF145 are not widely expressed. -
Involvement in disease
Defects in VEGFA are a cause of susceptibility to microvascular complications of diabetes type 1 (MVCD1) [MIM:603933]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. -
Sequence similarities
Belongs to the PDGF/VEGF growth factor family. -
Cellular localization
Secreted. VEGF121 is acidic and freely secreted. VEGF165 is more basic, has heparin-binding properties and, although a signicant proportion remains cell-associated, most is freely secreted. VEGF189 is very basic, it is cell-associated after secretion and is bound avidly by heparin and the extracellular matrix, although it may be released as a soluble form by heparin, heparinase or plasmin. - Information by UniProt
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Database links
- Entrez Gene: 7422 Human
- Omim: 192240 Human
- SwissProt: P15692 Human
- Unigene: 73793 Human
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Alternative names
- Folliculostellate cell-derived growth factor antibody
- Glioma-derived endothelial cell mitogen antibody
- MGC70609 antibody
see all
Images
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Western blot - Anti-VEGFA antibody [16F1] (ab155944)All lanes : Anti-VEGFA antibody [16F1] (ab155944) at 1 µg/ml
Lane 1 : VEGFA transfected 293T cell culture supernatant
Lane 2 : Recombinant VEGFA protein
Lysates/proteins at 0.5 µg per lane.
4-12% HCl -
![Sandwich ELISA - Anti-VEGFA antibody [16F1] (ab155944)](https://www.abcam.com/ps/products/155/ab155944/Images/ab155944-163215-anti-vegfa-antibody-16f1-sandwich-elisa.jpg)
Sandwich ELISA - Anti-VEGFA antibody [16F1] (ab155944)The plate was coated with 100 μL ab155944 at 1, 3, 4, 5 7 and 9 μg/mL in DPBS for 12 - 18 hours at 4°C. After washing the plate was blocked with 300 μL per well of 4% BSA / 5% sucrose in DPBS for 1 hour at room temperature.
Recombinant human VEGF protein (50 μL /well) was added at 2000, 1000, 500, 250, 125, 62.5, 31.25, and 0 pg/mL for 2 hours at room temperature.
After washing the plate was incubated with ab155944 at 0.5 μg /Ml for 1 hour at room temperature.
Detection was with Streptavidin-HRP / TMB.
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab155944 has been referenced in 7 publications.
- Yu X et al. AEG-1 Contributes to Metastasis in Hypoxia-Related Ovarian Cancer by Modulating the HIF-1alpha/NF-kappaB/VEGF Pathway. Biomed Res Int 2018:3145689 (2018). PubMed: 29770329
- Zhao X et al. MicroRNA-205 is downregulated in hepatocellular carcinoma and inhibits cell growth and metastasis via directly targeting vascular endothelial growth factor A. Oncol Lett 16:2207-2214 (2018). PubMed: 30008920
- Zhu D et al. miR-1 inhibits the progression of colon cancer by regulating the expression of vascular endothelial growth factor. Oncol Rep 40:589-598 (2018). PubMed: 29845255
- Liu WL MicroRNA-9 inhibits retinal neovascularization in rats with diabetic retinopathy by targeting vascular endothelial growth factor A. J Cell Biochem N/A:N/A (2018). PubMed: 30485520
- Li J et al. microRNA-497 overexpression decreases proliferation, migration and invasion of human retinoblastoma cells via targeting vascular endothelial growth factor A. Oncol Lett 13:5021-5027 (2017). PubMed: 28588740
- Zhou J et al. Protective role of microRNA-29a in denatured dermis and skin fibroblast cells after thermal injury. Biol Open N/A:N/A (2016). WB ; Mouse . PubMed: 26794609
- Sano M et al. Lymphangiogenesis and angiogenesis in abdominal aortic aneurysm. PLoS One 9:e89830 (2014). IHC-P ; Human . PubMed: 24651519