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    vegfa-antibody-16f1-ab155944.pdf

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Cardiovascular Angiogenesis Growth Factors VEGF VEGF
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Anti-VEGFA antibody [16F1] (ab155944)

  • Datasheet
  • SDS
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Western blot - Anti-VEGFA antibody [16F1] (ab155944)
  • Sandwich ELISA - Anti-VEGFA antibody [16F1] (ab155944)

Key features and details

  • Mouse monoclonal [16F1] to VEGFA
  • Suitable for: WB, Sandwich ELISA
  • Reacts with: Human, Recombinant fragment
  • Isotype: IgG2a

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Overview

  • Product name

    Anti-VEGFA antibody [16F1]
    See all VEGFA primary antibodies
  • Description

    Mouse monoclonal [16F1] to VEGFA
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, Sandwich ELISAmore details
  • Species reactivity

    Reacts with: Human, Recombinant fragment
  • Immunogen

    Recombinant full length Human VEGFA expressed in E-coli (P15692)

  • Positive control

    • Human VEGFA recombinant protein; Human VEGFA transfected 293T cells.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    Constituent: 100% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    16F1
  • Isotype

    IgG2a
  • Light chain type

    kappa
  • Research areas

    • Cardiovascular
    • Angiogenesis
    • Growth Factors
    • VEGF
    • VEGF
    • Signal Transduction
    • Growth Factors/Hormones
    • VEGF
    • Cancer
    • Growth factors
    • VEGF
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG2a, kappa monoclonal [MG2a-53] - Isotype control (ab18415)
  • Positive Controls

    • Recombinant human VEGFA protein (ab50078)
  • Recombinant Protein

    • Recombinant Human VEGFA protein (ab55566)
  • Related Products

    • Human VEGF ELISA Kit (ab100662)
    • Human VEGF ELISA Kit (ab100663)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab155944 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use a concentration of 1 µg/ml.
Sandwich ELISA
Use a concentration of 1 µg/ml.

Typical dilutions for sandwich ELISA include 1µg/ml for coating and 0.125-0.5µg/ml for detection.

Can be paired for Sandwich ELISA with Rabbit polyclonal to VEGFA (Biotin) (ab83132). To detect hVEGF by sandwich ELISA (using 100 µl/well antibody solution) a concentration of 0.5 - 2.0 µg/ml of this antibody as Detection is required. This antigen affinity purified antibody, paired with the above recommended pair, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hVEGF (ab9571).

Notes
WB
Use a concentration of 1 µg/ml.
Sandwich ELISA
Use a concentration of 1 µg/ml.

Typical dilutions for sandwich ELISA include 1µg/ml for coating and 0.125-0.5µg/ml for detection.

Can be paired for Sandwich ELISA with Rabbit polyclonal to VEGFA (Biotin) (ab83132). To detect hVEGF by sandwich ELISA (using 100 µl/well antibody solution) a concentration of 0.5 - 2.0 µg/ml of this antibody as Detection is required. This antigen affinity purified antibody, paired with the above recommended pair, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hVEGF (ab9571).

Target

  • Function

    Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth.
  • Tissue specificity

    Isoform VEGF189, isoform VEGF165 and isoform VEGF121 are widely expressed. Isoform VEGF206 and isoform VEGF145 are not widely expressed.
  • Involvement in disease

    Defects in VEGFA are a cause of susceptibility to microvascular complications of diabetes type 1 (MVCD1) [MIM:603933]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis.
  • Sequence similarities

    Belongs to the PDGF/VEGF growth factor family.
  • Cellular localization

    Secreted. VEGF121 is acidic and freely secreted. VEGF165 is more basic, has heparin-binding properties and, although a signicant proportion remains cell-associated, most is freely secreted. VEGF189 is very basic, it is cell-associated after secretion and is bound avidly by heparin and the extracellular matrix, although it may be released as a soluble form by heparin, heparinase or plasmin.
  • Target information above from: UniProt accession P15692 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 7422 Human
    • Omim: 192240 Human
    • SwissProt: P15692 Human
    • Unigene: 73793 Human
    • Alternative names

      • Folliculostellate cell-derived growth factor antibody
      • Glioma-derived endothelial cell mitogen antibody
      • MGC70609 antibody
      • MVCD1 antibody
      • Vascular endothelial growth factor A antibody
      • vascular endothelial growth factor A121 antibody
      • vascular endothelial growth factor A165 antibody
      • vascular endothelial growth factor antibody
      • Vascular permeability factor antibody
      • VEGF A antibody
      • Vegf antibody
      • VEGF-A antibody
      • VEGF120 antibody
      • Vegfa antibody
      • VEGFA_HUMAN antibody
      • VPF antibody
      see all

    Images

    • Western blot - Anti-VEGFA antibody [16F1] (ab155944)
      Western blot - Anti-VEGFA antibody [16F1] (ab155944)
      All lanes : Anti-VEGFA antibody [16F1] (ab155944) at 1 µg/ml

      Lane 1 : VEGFA transfected 293T cell culture supernatant
      Lane 2 : Recombinant VEGFA protein

      Lysates/proteins at 0.5 µg per lane.


      4-12% HCl
    • Sandwich ELISA - Anti-VEGFA antibody [16F1] (ab155944)
      Sandwich ELISA - Anti-VEGFA antibody [16F1] (ab155944)

      The plate was coated with 100 μL ab155944 at 1, 3, 4, 5 7 and 9 μg/mL in DPBS for 12 - 18 hours at 4°C. After washing the plate was blocked with 300 μL per well of 4% BSA / 5% sucrose in DPBS for 1 hour at room temperature.

      Recombinant human VEGF protein (50 μL /well) was added at 2000, 1000, 500, 250, 125, 62.5, 31.25, and 0 pg/mL for 2 hours at room temperature. 

      After washing the plate was incubated with ab155944 at 0.5 μg /Ml for 1 hour at room temperature.

      Detection was with Streptavidin-HRP / TMB.

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (7)

    Publishing research using ab155944? Please let us know so that we can cite the reference in this datasheet.

    ab155944 has been referenced in 7 publications.

    • Yu X  et al. AEG-1 Contributes to Metastasis in Hypoxia-Related Ovarian Cancer by Modulating the HIF-1alpha/NF-kappaB/VEGF Pathway. Biomed Res Int 2018:3145689 (2018). PubMed: 29770329
    • Zhao X  et al. MicroRNA-205 is downregulated in hepatocellular carcinoma and inhibits cell growth and metastasis via directly targeting vascular endothelial growth factor A. Oncol Lett 16:2207-2214 (2018). PubMed: 30008920
    • Zhu D  et al. miR-1 inhibits the progression of colon cancer by regulating the expression of vascular endothelial growth factor. Oncol Rep 40:589-598 (2018). PubMed: 29845255
    • Liu WL MicroRNA-9 inhibits retinal neovascularization in rats with diabetic retinopathy by targeting vascular endothelial growth factor A. J Cell Biochem N/A:N/A (2018). PubMed: 30485520
    • Li J  et al. microRNA-497 overexpression decreases proliferation, migration and invasion of human retinoblastoma cells via targeting vascular endothelial growth factor A. Oncol Lett 13:5021-5027 (2017). PubMed: 28588740
    • Zhou J  et al. Protective role of microRNA-29a in denatured dermis and skin fibroblast cells after thermal injury. Biol Open N/A:N/A (2016). WB ; Mouse . PubMed: 26794609
    • Sano M  et al. Lymphangiogenesis and angiogenesis in abdominal aortic aneurysm. PLoS One 9:e89830 (2014). IHC-P ; Human . PubMed: 24651519

    Customer reviews and Q&As

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