Anti-VEGFA antibody [6D3] (ab36424)

Submit a review Q&A (1)References (2)

Overview

  • Product name

    Anti-VEGFA antibody [6D3]
    See all VEGFA primary antibodies

  • Description

    Mouse monoclonal [6D3] to VEGFA

  • Host species

    Mouse

  • Specificity

    Recognizes vascular endothelial growth factor A precursor (VEGFA).

  • Tested applications

    Suitable for: WB, ELISA, Neutralisingmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant full length protein corresponding to Human VEGFA.

  • Positive control

    • Recombinant VEGF165.

Applications

Our Abpromise guarantee covers the use of ab36424 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2 - 10 µg/ml. Predicted molecular weight: 45 kDa.
ELISA Use a concentration of 1 - 15 µg/ml.
Neutralising Use at an assay dependent dilution. Has been shown to block the VEGFA induced proliferation of ACE cell at a 1:6 molar ratio of ligand to antibody. Inhibits the binding of VEGF A to the VEGF receptors 1 (Flt-1) and 2 (KDR).

Target

  • Function

    Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth.

  • Tissue specificity

    Isoform VEGF189, isoform VEGF165 and isoform VEGF121 are widely expressed. Isoform VEGF206 and isoform VEGF145 are not widely expressed.

  • Involvement in disease

    Defects in VEGFA are a cause of susceptibility to microvascular complications of diabetes type 1 (MVCD1) [MIM:603933]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis.

  • Sequence similarities

    Belongs to the PDGF/VEGF growth factor family.

  • Cellular localization

    Secreted. VEGF121 is acidic and freely secreted. VEGF165 is more basic, has heparin-binding properties and, although a signicant proportion remains cell-associated, most is freely secreted. VEGF189 is very basic, it is cell-associated after secretion and is bound avidly by heparin and the extracellular matrix, although it may be released as a soluble form by heparin, heparinase or plasmin.
  • Information by UniProt

  • Database links

    • Alternative names

      • Folliculostellate cell-derived growth factor antibody
      • Glioma-derived endothelial cell mitogen antibody
      • MGC70609 antibody
      • MVCD1 antibody
      • Vascular endothelial growth factor A antibody
      • vascular endothelial growth factor A121 antibody
      • vascular endothelial growth factor A165 antibody
      • vascular endothelial growth factor antibody
      • Vascular permeability factor antibody
      • VEGF A antibody
      • Vegf antibody
      • VEGF-A antibody
      • VEGF120 antibody
      • Vegfa antibody
      • VEGFA_HUMAN antibody
      • VPF antibody
      see all

    References

    This product has been referenced in:

    • Hudson EP  et al. Multiplex epitope mapping using bacterial surface display reveals both linear and conformational epitopes. Sci Rep 2:706 (2012). Read more (PubMed: 23050090) »
    • Rossi SL  et al. Histological and functional benefit following transplantation of motor neuron progenitors to the injured rat spinal cord. PLoS One 5:e11852 (2010). Blocking ; Human . Read more (PubMed: 20686613) »
    See all 2 Publications for this product

    Publishing research using ab36424? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    Question

    I would be interested in knowing anything you could share about the reactivity of the two below Ab with Rat and Cyno.  Thank you for your help.

    Read More
    Answer

    Thank you for your reply.

    These products were created using full length VEGF recombinant proteins. We are not currently aware of the exact epitopes recognized by the antibodies. I did run alignments of the human VEGF protein to both rat and cynomolgus. The results of these alignments were as follows:

    Human VEGF (http://www.uniprot.org/uniprot/P15692) to Rat (http://www.uniprot.org/uniprot/P16612):83.6% identity in 232 aa overlap (1-232:1-214).

    Human VEGF (http://www.uniprot.org/uniprot/P15692)to Cynomolgus (http://www.uniprot.org/uniprot/Q9XT61):85.7% identity in 119 aa overlap(114-232:1-102). Please note that this lowered identity is result of a 17aa fragment missing in cynomolgus, the remaining amino acids are 100% aligned.

    While we have not yet tested these product in against these species, based on the sequence homolgy we believe that there is a good chance that these product will recognize both species.For UNTESTED species and/or applications, we have established a testing discount program. Here is a brief description of how it works:

    The testing discount program is for customers who like to use an antibody/protein on an untested species/application. You would purchase the antibody at full price, test it and submit an Abreview with your data (positive or negative). On your next order you will receive a discount for ONE antibody at the full price (100%) of the antibody you have tested. The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.


    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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