Anti-VEGFA antibody [6D3] (ab36424)

Submit a review Q&A (1)References (3)

Key features and details

  • Mouse monoclonal [6D3] to VEGFA
  • Reacts with: Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-VEGFA antibody [6D3]
    See all VEGFA primary antibodies

  • Description

    Mouse monoclonal [6D3] to VEGFA

  • Host species

    Mouse

  • Specificity

    Recognizes vascular endothelial growth factor A precursor (VEGFA).

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant full length protein corresponding to Human VEGFA.

  • Positive control

    • Recombinant VEGF165.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Target

  • Function

    Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth.

  • Tissue specificity

    Isoform VEGF189, isoform VEGF165 and isoform VEGF121 are widely expressed. Isoform VEGF206 and isoform VEGF145 are not widely expressed.

  • Involvement in disease

    Defects in VEGFA are a cause of susceptibility to microvascular complications of diabetes type 1 (MVCD1) [MIM:603933]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis.

  • Sequence similarities

    Belongs to the PDGF/VEGF growth factor family.

  • Cellular localization

    Secreted. VEGF121 is acidic and freely secreted. VEGF165 is more basic, has heparin-binding properties and, although a signicant proportion remains cell-associated, most is freely secreted. VEGF189 is very basic, it is cell-associated after secretion and is bound avidly by heparin and the extracellular matrix, although it may be released as a soluble form by heparin, heparinase or plasmin.
  • Information by UniProt

  • Database links

    • Alternative names

      • Folliculostellate cell-derived growth factor antibody
      • Glioma-derived endothelial cell mitogen antibody
      • MGC70609 antibody
      • MVCD1 antibody
      • Vascular endothelial growth factor A antibody
      • vascular endothelial growth factor A121 antibody
      • vascular endothelial growth factor A165 antibody
      • vascular endothelial growth factor antibody
      • Vascular permeability factor antibody
      • VEGF A antibody
      • Vegf antibody
      • VEGF-A antibody
      • VEGF120 antibody
      • Vegfa antibody
      • VEGFA_HUMAN antibody
      • VPF antibody
      see all

    References (3)

    Publishing research using ab36424? Please let us know so that we can cite the reference in this datasheet.

    ab36424 has been referenced in 3 publications.

    • Jiang Y  et al. Overexpression of Limb-Bud and Heart (LBH) promotes angiogenesis in human glioma via VEGFA-mediated ERK signalling under hypoxia. EBioMedicine 48:36-48 (2019). PubMed: 31631037
    • Hudson EP  et al. Multiplex epitope mapping using bacterial surface display reveals both linear and conformational epitopes. Sci Rep 2:706 (2012). PubMed: 23050090
    • Rossi SL  et al. Histological and functional benefit following transplantation of motor neuron progenitors to the injured rat spinal cord. PLoS One 5:e11852 (2010). Blocking ; Human . PubMed: 20686613

    Customer reviews and Q&As

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    Question

    I would be interested in knowing anything you could share about the reactivity of the two below Ab with Rat and Cyno.  Thank you for your help.

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    Answer

    Thank you for your reply.

    These products were created using full length VEGF recombinant proteins. We are not currently aware of the exact epitopes recognized by the antibodies. I did run alignments of the human VEGF protein to both rat and cynomolgus. The results of these alignments were as follows:

    Human VEGF (http://www.uniprot.org/uniprot/P15692) to Rat (http://www.uniprot.org/uniprot/P16612):83.6% identity in 232 aa overlap (1-232:1-214).

    Human VEGF (http://www.uniprot.org/uniprot/P15692)to Cynomolgus (http://www.uniprot.org/uniprot/Q9XT61):85.7% identity in 119 aa overlap(114-232:1-102). Please note that this lowered identity is result of a 17aa fragment missing in cynomolgus, the remaining amino acids are 100% aligned.

    While we have not yet tested these product in against these species, based on the sequence homolgy we believe that there is a good chance that these product will recognize both species.For UNTESTED species and/or applications, we have established a testing discount program. Here is a brief description of how it works:

    The testing discount program is for customers who like to use an antibody/protein on an untested species/application. You would purchase the antibody at full price, test it and submit an Abreview with your data (positive or negative). On your next order you will receive a discount for ONE antibody at the full price (100%) of the antibody you have tested. The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.


    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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