Overview

  • Product name
  • Description
    Rabbit polyclonal to VEGFA
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human VEGFA aa 130-143. The exact sequence is proprietary.
    Sequence:

    CRPKKDRARQEKKS


    Database link: P15692

  • Positive control
    • IHC-P: Human liver cancer tissue. WB: Hela, Jurkat, HEK-293T, HT29, MDA-MB-231, A549, SK-OV-3 and 231 cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab183100 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/450 - 1/2000. Predicted molecular weight: 27 kDa.
IHC-P 1/25 - 1/100.

Target

  • Function
    Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth.
  • Tissue specificity
    Isoform VEGF189, isoform VEGF165 and isoform VEGF121 are widely expressed. Isoform VEGF206 and isoform VEGF145 are not widely expressed.
  • Involvement in disease
    Defects in VEGFA are a cause of susceptibility to microvascular complications of diabetes type 1 (MVCD1) [MIM:603933]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis.
  • Sequence similarities
    Belongs to the PDGF/VEGF growth factor family.
  • Cellular localization
    Secreted. VEGF121 is acidic and freely secreted. VEGF165 is more basic, has heparin-binding properties and, although a signicant proportion remains cell-associated, most is freely secreted. VEGF189 is very basic, it is cell-associated after secretion and is bound avidly by heparin and the extracellular matrix, although it may be released as a soluble form by heparin, heparinase or plasmin.
  • Information by UniProt
  • Database links
  • Alternative names
    • Folliculostellate cell-derived growth factor antibody
    • Glioma-derived endothelial cell mitogen antibody
    • MGC70609 antibody
    • MVCD1 antibody
    • Vascular endothelial growth factor A antibody
    • vascular endothelial growth factor A121 antibody
    • vascular endothelial growth factor A165 antibody
    • vascular endothelial growth factor antibody
    • Vascular permeability factor antibody
    • VEGF A antibody
    • Vegf antibody
    • VEGF-A antibody
    • VEGF120 antibody
    • Vegfa antibody
    • VEGFA_HUMAN antibody
    • VPF antibody
    see all

Images

  • Immunohistochemical analysis of paraffin embedded Human liver cancer tissue labeling VEGFA with ab183100 at 1/25. Image on the right is treated with the synthetic peptide.

  • All lanes : Anti-VEGFA antibody (ab183100) at 1/450 dilution

    Lane 1 : Hela cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : 293T cell lysate
    Lane 4 : 231 cell lysate

    Lysates/proteins at 40 µg per lane.

    Secondary
    All lanes : Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution

    Predicted band size: 27 kDa


    Exposure time: 2 minutes
  • All lanes : Anti-VEGFA antibody (ab183100) at 1/1000 dilution

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
    Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
    Lane 3 : HepG2 (human liver hepatocellular carcinoma cell line) cell lysate
    Lane 4 : HT29 cell lysate
    Lane 5 : MDA-MB-231 (human breast adenocarcinoma cell line) cell lysate
    Lane 6 : A549 (human lung carcinoma cell line) cell lysate
    Lane 7 : SK-OV-3 (human ovarian cancer cell line) cell lysate

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : Goat anti Rabbit IgG - H&L (HRP) at 1/100 dilution

    Developed using the ECL technique.

    Predicted band size: 27 kDa

References

This product has been referenced in:
  • Wei X  et al. GMDS knockdown impairs cell proliferation and survival in human lung adenocarcinoma. BMC Cancer 18:600 (2018). Read more (PubMed: 29843634) »
  • Zheng J  et al. Evaluation of metastatic niches in distant organs after surgical removal of tumor-bearing lymph nodes. BMC Cancer 18:608 (2018). Read more (PubMed: 29848296) »
See all 8 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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