Overview

  • Product name
  • Description
    Rabbit polyclonal to VEGFA
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-FrFl, IHC-P, IHC-FoFr, IP, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human VEGFA aa 1-100 (N terminal). The exact sequence is proprietary.
    Database link: P15692

  • Positive control
    • Tumor cells in hemangiosarcomas IF/ICC: HepG2 cell line

Applications

Our Abpromise guarantee covers the use of ab39250 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FrFl Use at an assay dependent concentration.
IHC-P 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-FoFr 1/100.
IP Use at an assay dependent concentration.
ICC/IF Use a concentration of 1 µg/ml.

Target

  • Function
    Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth.
  • Tissue specificity
    Isoform VEGF189, isoform VEGF165 and isoform VEGF121 are widely expressed. Isoform VEGF206 and isoform VEGF145 are not widely expressed.
  • Involvement in disease
    Defects in VEGFA are a cause of susceptibility to microvascular complications of diabetes type 1 (MVCD1) [MIM:603933]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis.
  • Sequence similarities
    Belongs to the PDGF/VEGF growth factor family.
  • Cellular localization
    Secreted. VEGF121 is acidic and freely secreted. VEGF165 is more basic, has heparin-binding properties and, although a signicant proportion remains cell-associated, most is freely secreted. VEGF189 is very basic, it is cell-associated after secretion and is bound avidly by heparin and the extracellular matrix, although it may be released as a soluble form by heparin, heparinase or plasmin.
  • Information by UniProt
  • Database links
  • Alternative names
    • Folliculostellate cell-derived growth factor antibody
    • Glioma-derived endothelial cell mitogen antibody
    • MGC70609 antibody
    • MVCD1 antibody
    • Vascular endothelial growth factor A antibody
    • vascular endothelial growth factor A121 antibody
    • vascular endothelial growth factor A165 antibody
    • vascular endothelial growth factor antibody
    • Vascular permeability factor antibody
    • VEGF A antibody
    • Vegf antibody
    • VEGF-A antibody
    • VEGF120 antibody
    • Vegfa antibody
    • VEGFA_HUMAN antibody
    • VPF antibody
    see all

Images

  • ICC/IF image of ab39250 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab39250, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of human angiosarcoma stained with ab39250 at 1/100.

  • ab39250 staining VEGF in mouse brain tissue by Immunohistochemistry (Free floating sections). A heat mediated antigen retrieval step (100°C for 20 minutes) was performed using 10mM sodium citrate buffer, 0.05% Tween 20 pH 6.0. Samples were then incubated with the primary antibody at a 1/100 dilution for 72 hours at 4°C. An Alexa Fluor®488-conjugated goat anti-rabbit polyclonal was used as secondary antibody at a 1/500 dilution.

    See Abreview

References

This product has been referenced in:
  • Spina A  et al. NZ-GMP Approved Serum Improve hDPSC Osteogenic Commitment and Increase Angiogenic Factor Expression. Front Physiol 7:354 (2016). ICC/IF ; Human . Read more (PubMed: 27594842) »
  • Kim JS  et al. Characterization of degenerative human facet joints and facet joint capsular tissues. Osteoarthritis Cartilage 23:2242-2251 (2015). Read more (PubMed: 26117175) »
See all 8 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Question
Answer

Thank you for contacting us and your interst in our products.
As discussed over the phone, the Anti-VEGF antibody (ab39250) has not as yet been used in western blotting as you are hoping to do. This does not mean that it would not work, simply that we have not had any reports supporting it yet and would therefore not be able to guarentee it.
I'd be happy to offer you the testing discount if you wanted to try this antibody. This offer means that if you test the antibody in Western blotting and share the results with us through an Abreview (no matter whether positive or negative) you would be eligible for a free primary antibody of your choice from our catalogue (or the equivalent price off any product in our catalogue). More information on this offer can be found here:
www.abcam.com/collaborationdiscount
The only constraint to this offer is that the antibody to be tested must be purchased, tested, the Abreview submitted and the free primary antibody claimed within a 4 month period.
As discussed over the phone, here is the discount code:
xxxxxxx

Please quote this code in the "Additional comments" section when submitting your Abreview. Once the Abreview has been submitted the code will become activated and you can quote the code when placing the order for your free product. This code expires on the 26th of November 2012.
I hope this information has been of help. If you require any further information please do not hesitate to contact us again.

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Answer

ab39250 is against VEGFA (VEGF). We do not have any experimental data regarding the cross-reactivity of E2614 with VEGFB, VEGFC, or VEGFD. The percentage homology between the immunogen and the other variants of VEGF are as follows: VEGFB (P49765): 33% VEGFC (P49767): 60% VEGFD (O43915): 50%

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Application
Immunohistochemistry free floating
Sample
Mouse Tissue sections (brain)
Specification
brain

Ms. Christina Whiteus

Verified customer

Submitted Oct 15 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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