Anti-VEGFA antibody (ab46154)
- Datasheet
- References (228)
- Protocols
Overview
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Product nameAnti-VEGFA antibody
See all VEGFA primary antibodies -
DescriptionRabbit polyclonal to VEGFA
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Host speciesRabbit
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SpecificityNew batches of this antibody are no longer guaranteed in ICC/IF, IHC-P or IHC-Fr as they have not passed our stringent batch testing criteria. Please contact customer support for any specific queries. We recommend ab52917 as an alternative for ICC/IF and IHC-P.
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Tested applicationsSuitable for: WB, ELISAmore details
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Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Synthetic peptide corresponding to Human VEGFA aa 50-150 conjugated to Keyhole Limpet Haemocyanin (KLH).
(Peptide available asab46161) -
Positive control
- Purchase matching WB positive control:Recombinant Human VEGFA protein
- ab46154 gave a positive result in the following whole cell lysates: Recombinant Human VEGFA protein Recombinant Mouse VEGFA protein HCT116 whole cell lysate
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Control Peptide
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Immunohistochemistry kits
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab46154 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
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| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 23 kDa (predicted molecular weight: 27 kDa). We recommend Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody. |
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| ELISA | Use at an assay dependent concentration. |
Target
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FunctionGrowth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth.
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Tissue specificityIsoform VEGF189, isoform VEGF165 and isoform VEGF121 are widely expressed. Isoform VEGF206 and isoform VEGF145 are not widely expressed.
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Involvement in diseaseDefects in VEGFA are a cause of susceptibility to microvascular complications of diabetes type 1 (MVCD1) [MIM:603933]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis.
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Sequence similaritiesBelongs to the PDGF/VEGF growth factor family.
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Cellular localizationSecreted. VEGF121 is acidic and freely secreted. VEGF165 is more basic, has heparin-binding properties and, although a signicant proportion remains cell-associated, most is freely secreted. VEGF189 is very basic, it is cell-associated after secretion and is bound avidly by heparin and the extracellular matrix, although it may be released as a soluble form by heparin, heparinase or plasmin.
- Information by UniProt
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Database links
- Entrez Gene: 7422 Human
- Entrez Gene: 22339 Mouse
- Entrez Gene: 83785 Rat
- Omim: 192240 Human
- SwissProt: P15692 Human
- SwissProt: Q00731 Mouse
- SwissProt: P16612 Rat
- Unigene: 73793 Human
see all -
Alternative names
- Folliculostellate cell-derived growth factor antibody
- Glioma-derived endothelial cell mitogen antibody
- MGC70609 antibody
see all
Images
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Western blot - Anti-VEGFA antibody (ab46154)Anti-VEGFA antibody (ab46154) at 1 µg/ml + HCT116 whole cell lysate at 20 µg
Secondary
Rabbit IgG secondary antibody at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 27 kDa
Observed band size: 23 kDa why is the actual band size different from the predicted?
Exposure time: 2 minutesThis blot was produced using a 10% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab46154 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
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ELISA - Anti-VEGFA antibody (ab46154)Ab46154 was tested using indirect ELISA. The wells were coated with peptide (1 µg x mL-1 at 100 µL per well) overnight at 4°C, followed by a 1% fat-free milk blocking step for 1 hour at room temperature. The primary antibody (ab46154) was added at a range of dilutions (50 µL per well) for 1 hour at room temperature. Ab97080 (Goat Anti-Rabbit IgG H&L-HRP) was used as a secondary antibody at 1:50.000 dilution for 1 hour at room temperature and signal was developed with TMB substrate.
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Western blot - Anti-VEGFA antibody (ab46154)All lanes : Anti-VEGFA antibody (ab46154) at 1 µg/ml
Lane 1 :Recombinant Human VEGFA protein (ab204773)
Lane 2 :Recombinant mouse VEGFA protein (Active) (ab185265)
Lysates/proteins at 0.1 µg per lane.
Secondary
All lanes : Rabbit IgG secondary antibody at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 27 kDa
Observed band size: 23 kDa why is the actual band size different from the predicted?
Exposure time: 10 secondsThis blot was produced using a 10% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab46154 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
References
This product has been referenced in:
- Zou X et al. Endogenous hormone 2-methoxyestradiol suppresses venous hypertension-induced angiogenesis through up- and down-regulating p53 and id-1. J Cell Mol Med 22:957-967 (2018). Read more (PubMed: 29193609) »
- Nürnberg C et al. Dataset on the activation of Müller cells through macrophages upon hypoxia in the retina. Data Brief 16:489-500 (2018). WB ; Dog . Read more (PubMed: 29255783) »
