Anti-VEGFA antibody (ab46154)
Key features and details
- Rabbit polyclonal to VEGFA
- Suitable for: WB, ELISA
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-VEGFA antibody
See all VEGFA primary antibodies -
Description
Rabbit polyclonal to VEGFA -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseHuman
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Immunogen
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Positive control
- Purchase matching WB positive control:Recombinant Human VEGFA protein
- ab46154 gave a positive result in the following whole cell lysates: Recombinant Human VEGFA protein Recombinant Mouse VEGFA protein HCT116 whole cell lysate
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General notes
New batches of this antibody are no longer guaranteed in ICC/IF, IHC-P or IHC-Fr as they have not passed our stringent batch testing criteria. Please contact customer support for any specific queries. We recommend ab52917 as an alternative for ICC/IF and IHC-P.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Control Peptide
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Immunizing Peptide (Blocking)
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Immunohistochemistry kits
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
- Human VEGF ELISA Kit (ab100662)
- Human VEGF ELISA Kit (ab100663)
- Mouse VEGF ELISA Kit (ab100751)
- Prestained Protein Ladder - Broad molecular weight (10-245 kDa) (ab116028)
- Y-27632 dihydrochloride, Rho kinase inhibitor (ab120129)
- HA-1077 (Fasudil), ROCK2 inhibitor (ab120306)
- Mouse VEGFA Matched Antibody Pair Kit (ab220118)
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab46154 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| WB |
Mouse
Human
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| All applications |
Rat
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| Application | Abreviews | Notes |
|---|---|---|
| WB | (16) |
Use a concentration of 1 µg/ml. Detects a band of approximately 23 kDa (predicted molecular weight: 27 kDa).Can be blocked with Human VEGFA peptide (ab46160).
We recommend Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody. |
| ELISA |
Use at an assay dependent concentration.
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| Notes |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 23 kDa (predicted molecular weight: 27 kDa).Can be blocked with Human VEGFA peptide (ab46160). We recommend Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody. |
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ELISA
Use at an assay dependent concentration. |
Target
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Function
Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth. -
Tissue specificity
Isoform VEGF189, isoform VEGF165 and isoform VEGF121 are widely expressed. Isoform VEGF206 and isoform VEGF145 are not widely expressed. -
Involvement in disease
Defects in VEGFA are a cause of susceptibility to microvascular complications of diabetes type 1 (MVCD1) [MIM:603933]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. -
Sequence similarities
Belongs to the PDGF/VEGF growth factor family. -
Cellular localization
Secreted. VEGF121 is acidic and freely secreted. VEGF165 is more basic, has heparin-binding properties and, although a signicant proportion remains cell-associated, most is freely secreted. VEGF189 is very basic, it is cell-associated after secretion and is bound avidly by heparin and the extracellular matrix, although it may be released as a soluble form by heparin, heparinase or plasmin. - Information by UniProt
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Database links
- Entrez Gene: 7422 Human
- Entrez Gene: 22339 Mouse
- Entrez Gene: 83785 Rat
- Omim: 192240 Human
- SwissProt: P15692 Human
- SwissProt: Q00731 Mouse
- SwissProt: P16612 Rat
- Unigene: 73793 Human
see all -
Alternative names
- Folliculostellate cell-derived growth factor antibody
- Glioma-derived endothelial cell mitogen antibody
- MGC70609 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGFA antibody (ab46154)Courtesy of Feng Y et al. Sci Rep. 2017; 7: 44810. doi: 10.1038/srep44810 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/.
Immunohistochemical analysis of adult proestrous mouse ovarian follicles staining CYP19/aromatase with ab35604, and VEGFA with ab46154. Staining of follicles at different stages using specific markers (upper panels) together with histological pictures using hematoxylin and eosin staining (lower panels).
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Western blot - Anti-VEGFA antibody (ab46154)Anti-VEGFA antibody (ab46154) at 1 µg/ml + HCT116 whole cell lysate at 20 µg
Secondary
Rabbit IgG secondary antibody at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 27 kDa
Observed band size: 23 kDa why is the actual band size different from the predicted?
Exposure time: 2 minutesThis blot was produced using a 10% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab46154 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
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ELISA - Anti-VEGFA antibody (ab46154)Ab46154 was tested using indirect ELISA. The wells were coated with peptide (1 µg x mL-1 at 100 µL per well) overnight at 4°C, followed by a 1% fat-free milk blocking step for 1 hour at room temperature. The primary antibody (ab46154) was added at a range of dilutions (50 µL per well) for 1 hour at room temperature. Ab97080 (Goat Anti-Rabbit IgG H&L-HRP) was used as a secondary antibody at 1:50.000 dilution for 1 hour at room temperature and signal was developed with TMB substrate.
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Western blot - Anti-VEGFA antibody (ab46154)All lanes : Anti-VEGFA antibody (ab46154) at 1 µg/ml
Lane 1 : Recombinant Human VEGFA protein (His tag) (ab204773)
Lane 2 :Recombinant mouse VEGFA protein (Active) (ab185265)
Lysates/proteins at 0.1 µg per lane.
Secondary
All lanes : Rabbit IgG secondary antibody at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 27 kDa
Observed band size: 23 kDa why is the actual band size different from the predicted?
Exposure time: 10 secondsThis blot was produced using a 10% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab46154 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (437)
ab46154 has been referenced in 437 publications.
- Chen H et al. Regeneration of pulpo-dentinal-like complex by a group of unique multipotent CD24a+ stem cells. Sci Adv 6:eaay1514 (2020). PubMed: 32284993
- Jing L et al. Protective effects of two novel nitronyl nitroxide radicals on heart failure induced by hypobaric hypoxia. Life Sci 248:116481 (2020). PubMed: 31102744
- Wang Y et al. The role of a drug-loaded poly (lactic co-glycolic acid) (PLGA) copolymer stent in the treatment of ovarian cancer. Cancer Biol Med 17:237-250 (2020). PubMed: 32296591
- García-Bonilla M et al. Neocortical tissue recovery in severe congenital obstructive hydrocephalus after intraventricular administration of bone marrow-derived mesenchymal stem cells. Stem Cell Res Ther 11:121 (2020). PubMed: 32183876
- Nguyen VT et al. Effect of Chemically Induced Hypoxia on Osteogenic and Angiogenic Differentiation of Bone Marrow Mesenchymal Stem Cells and Human Umbilical Vein Endothelial Cells in Direct Coculture. Cells 9:N/A (2020). PubMed: 32204578