• Product name
  • Description
    Rabbit polyclonal to VEGFA
  • Host species
  • Tested applications
    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant full length protein corresponding to Mouse VEGFA. Recombinant mouse VEGF164
    Database link: Q00731

  • Positive control
    • SaOS2 cells. Human pancreas tissue.



Our Abpromise guarantee covers the use of ab51745 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 1 µg/ml. Predicted molecular weight: 27 kDa. The detection limit for recombinant murine VEGF-A is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
ELISA Use a concentration of 1 - 10 µg/ml.
IHC-P Use a concentration of 2 - 10 µg/ml.


  • Function
    Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth.
  • Tissue specificity
    Isoform VEGF189, isoform VEGF165 and isoform VEGF121 are widely expressed. Isoform VEGF206 and isoform VEGF145 are not widely expressed.
  • Involvement in disease
    Defects in VEGFA are a cause of susceptibility to microvascular complications of diabetes type 1 (MVCD1) [MIM:603933]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis.
  • Sequence similarities
    Belongs to the PDGF/VEGF growth factor family.
  • Cellular localization
    Secreted. VEGF121 is acidic and freely secreted. VEGF165 is more basic, has heparin-binding properties and, although a signicant proportion remains cell-associated, most is freely secreted. VEGF189 is very basic, it is cell-associated after secretion and is bound avidly by heparin and the extracellular matrix, although it may be released as a soluble form by heparin, heparinase or plasmin.
  • Information by UniProt
  • Database links
  • Alternative names
    • Folliculostellate cell-derived growth factor antibody
    • Glioma-derived endothelial cell mitogen antibody
    • MGC70609 antibody
    • MVCD1 antibody
    • Vascular endothelial growth factor A antibody
    • vascular endothelial growth factor A121 antibody
    • vascular endothelial growth factor A165 antibody
    • vascular endothelial growth factor antibody
    • Vascular permeability factor antibody
    • VEGF A antibody
    • Vegf antibody
    • VEGF-A antibody
    • VEGF120 antibody
    • Vegfa antibody
    • VEGFA_HUMAN antibody
    • VPF antibody
    see all


  • Staining of VEGF-A in paraffin-embedded mouse ventricular zone of the CNS (E17) and a perinecrotic area of a human glioblastom.

    Top panel: Mouse E17: ventricular zone of the CNS.

    Bottom panel: Human glioblastoma: perinecrotic area.

    The experiments were performed by Dr. Till Acker and Prof. K.H. Plate, Neurological Institute, Neuropathology, Deutschordenstr. 45, 60528 Frankfurt, Germany

  • All lanes : Anti-VEGFA antibody (ab51745)

    Lane 1 : recombinant mouse VEGF164
    Lane 2 : Recombinant human VEGF165
    Lane 3 : Recombinant human PlGF-1
    Lane 4 : Recombinant rat VEGF-C

    Predicted band size: 27 kDa


This product has been referenced in:
  • Lu T  et al. Blockade of ONECUT2 expression in ovarian cancer inhibited tumor cell proliferation, migration, invasion and angiogenesis. Cancer Sci 109:2221-2234 (2018). Read more (PubMed: 29737581) »
  • Lun W  et al. MiR-218 regulates epithelial-mesenchymal transition and angiogenesis in colorectal cancer via targeting CTGF. Cancer Cell Int 18:83 (2018). Read more (PubMed: 29977158) »
See all 31 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Mouse Tissue sections (Tumor tissue)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris EDTA pH8.0
Tumor tissue
Blocking step
Serum as blocking agent for 15 minute(s) · Concentration: 50µg/mL · Temperature: 37°C

Abcam user community

Verified customer

Submitted Apr 23 2015


Thank you for contacting us.

The rabbit anti-mouse VEGF-A antibody ab51745 did not cross react with human VEGF-C in Western blotting (reduced, non-reduced). According to our results the rabbit anti-rat VEGF-C ab9546 cross reacts with human VEGF-C but not with human VEGF165.

Regarding ab105219 I am sorry, we have not tested this antibody for reactivity with VEGFC.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Western blot
Mouse Tissue lysate - whole (Melanoma cancer)
Loading amount
30 µg
Melanoma cancer
Gel Running Conditions
Non-reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C

Ms. Seontae Kim

Verified customer

Submitted Jan 02 2013


Thank you for your enquiry. The MW of recombinant mouse VEGF164 dimer produced in insect cells is about 48 kDa, but the monomer has a MW of about 24 kDa.. The predicted size of 27 kDa is probably those of VEGF164 produced in mammalian cells. This is because insect cells have a different glycosylation than mammalian cells. The glycosylation in insect cells is lower than those of mammalian cells. If your customer wants to detect the monomer, he has to run the SDS-PAGE under reducing conditions. I hope this information will be helpful. If there is anything else that I can help you with, please do not hesitate to contact me.

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