Anti-VEGFA antibody (ab51745)
Key features and details
- Rabbit polyclonal to VEGFA
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human, Recombinant fragment
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-VEGFA antibody
See all VEGFA primary antibodies -
Description
Rabbit polyclonal to VEGFA -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human, Recombinant fragment -
Immunogen
Recombinant full length protein corresponding to Mouse VEGFA. Recombinant mouse VEGF164
Database link: Q00731 -
Positive control
- SaOS2 cells. Mouse pancreas tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Lyophilized:The lyophilized IgG is stable at 4°C for at least one month and for greater than a year when kept at –20°C. When reconstituted in sterile water to a concentration of >0.5 mg/ml the antibody is stable for at least six weeks at 2-4°C. -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab51745 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
Use a concentration of 0.1 - 1 µg/ml. Predicted molecular weight: 27 kDa. The detection limit for recombinant murine VEGF-A is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
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| IHC-P | (1) |
Use a concentration of 2 - 10 µg/ml.
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| Notes |
|---|
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WB
Use a concentration of 0.1 - 1 µg/ml. Predicted molecular weight: 27 kDa. The detection limit for recombinant murine VEGF-A is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. |
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IHC-P
Use a concentration of 2 - 10 µg/ml. |
Target
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Function
Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth. -
Tissue specificity
Isoform VEGF189, isoform VEGF165 and isoform VEGF121 are widely expressed. Isoform VEGF206 and isoform VEGF145 are not widely expressed. -
Involvement in disease
Defects in VEGFA are a cause of susceptibility to microvascular complications of diabetes type 1 (MVCD1) [MIM:603933]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. -
Sequence similarities
Belongs to the PDGF/VEGF growth factor family. -
Cellular localization
Secreted. VEGF121 is acidic and freely secreted. VEGF165 is more basic, has heparin-binding properties and, although a signicant proportion remains cell-associated, most is freely secreted. VEGF189 is very basic, it is cell-associated after secretion and is bound avidly by heparin and the extracellular matrix, although it may be released as a soluble form by heparin, heparinase or plasmin. - Information by UniProt
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Database links
- Entrez Gene: 7422 Human
- Entrez Gene: 22339 Mouse
- Omim: 192240 Human
- SwissProt: P15692 Human
- SwissProt: Q96KJ0 Human
- SwissProt: Q00731 Mouse
- Unigene: 73793 Human
- Unigene: 282184 Mouse
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Alternative names
- Folliculostellate cell-derived growth factor antibody
- Glioma-derived endothelial cell mitogen antibody
- MGC70609 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGFA antibody (ab51745)
Staining of VEGF-A in paraffin-embedded mouse ventricular zone of the CNS (E17) and a perinecrotic area of a human glioblastom.
Top panel: Mouse E17: ventricular zone of the CNS.
Bottom panel: Human glioblastoma: perinecrotic area.
The experiments were performed by Dr. Till Acker and Prof. K.H. Plate, Neurological Institute, Neuropathology, Deutschordenstr. 45, 60528 Frankfurt, Germany
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Western blot - Anti-VEGFA antibody (ab51745)All lanes : Anti-VEGFA antibody (ab51745)
Lane 1 : recombinant mouse VEGF164
Lane 2 : Recombinant human VEGF165
Lane 3 : Recombinant human PlGF-1
Lane 4 : Recombinant rat VEGF-C
Predicted band size: 27 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (58)
ab51745 has been referenced in 58 publications.
- Xiong YW et al. Environmental exposure to cadmium impairs fetal growth and placental angiogenesis via GCN-2-mediated mitochondrial stress. J Hazard Mater 401:123438 (2021). PubMed: 32763717
- Pan Y et al. Dysfunction of Shh signaling activates autophagy to inhibit trophoblast motility in recurrent miscarriage. Exp Mol Med 53:52-66 (2021). PubMed: 33390589
- Cai G et al. Mesenchymal stem cell-derived exosome miR-542-3p suppresses inflammation and prevents cerebral infarction. Stem Cell Res Ther 12:2 (2021). PubMed: 33407827
- Li G et al. Long Non-Coding RNA JPX Contributes to Tumorigenesis by Regulating miR-5195-3p/VEGFA in Non-Small Cell Lung Cancer. Cancer Manag Res 13:1477-1489 (2021). PubMed: 33613033
- Zhang Y et al. Effect of intravitreal conbercept injection on VEGF-A and -B levels in the aqueous and vitreous humor of patients with proliferative diabetic retinopathy. Exp Ther Med 21:332 (2021). PubMed: 33732305