Anti-VEGFA antibody (ab51745)
Key features and details
- Rabbit polyclonal to VEGFA
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human, Recombinant fragment
- Isotype: IgG
Overview
-
Product name
Anti-VEGFA antibody
See all VEGFA primary antibodies -
Description
Rabbit polyclonal to VEGFA -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P MouseHumanWB Mouse
-
Immunogen
Recombinant full length protein corresponding to Mouse VEGFA. Recombinant mouse VEGF164
Database link: Q00731 -
Positive control
- SaOS2 cells. Mouse pancreas tissue.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
-
Form
Lyophilized:The lyophilized IgG is stable at 4°C for at least one month and for greater than a year when kept at –20°C. When reconstituted in sterile water to a concentration of >0.5 mg/ml the antibody is stable for at least six weeks at 2-4°C. -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab51745 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| IHC-P |
Mouse
Human
|
| WB |
Mouse
|
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
Use a concentration of 0.1 - 1 µg/ml. Predicted molecular weight: 27 kDa. The detection limit for recombinant murine VEGF-A is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
|
| IHC-P | (1) |
Use a concentration of 2 - 10 µg/ml.
|
| Notes |
|---|
|
WB
Use a concentration of 0.1 - 1 µg/ml. Predicted molecular weight: 27 kDa. The detection limit for recombinant murine VEGF-A is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. |
|
IHC-P
Use a concentration of 2 - 10 µg/ml. |
Target
-
Function
Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth. -
Tissue specificity
Isoform VEGF189, isoform VEGF165 and isoform VEGF121 are widely expressed. Isoform VEGF206 and isoform VEGF145 are not widely expressed. -
Involvement in disease
Defects in VEGFA are a cause of susceptibility to microvascular complications of diabetes type 1 (MVCD1) [MIM:603933]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. -
Sequence similarities
Belongs to the PDGF/VEGF growth factor family. -
Cellular localization
Secreted. VEGF121 is acidic and freely secreted. VEGF165 is more basic, has heparin-binding properties and, although a signicant proportion remains cell-associated, most is freely secreted. VEGF189 is very basic, it is cell-associated after secretion and is bound avidly by heparin and the extracellular matrix, although it may be released as a soluble form by heparin, heparinase or plasmin. - Information by UniProt
-
Database links
- Entrez Gene: 7422 Human
- Entrez Gene: 22339 Mouse
- Omim: 192240 Human
- SwissProt: P15692 Human
- SwissProt: Q00731 Mouse
- Unigene: 73793 Human
- Unigene: 282184 Mouse
-
Alternative names
- Folliculostellate cell-derived growth factor antibody
- Glioma-derived endothelial cell mitogen antibody
- MGC70609 antibody
see all
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGFA antibody (ab51745)
Staining of VEGF-A in paraffin-embedded mouse ventricular zone of the CNS (E17) and a perinecrotic area of a human glioblastom.
Top panel: Mouse E17: ventricular zone of the CNS.
Bottom panel: Human glioblastoma: perinecrotic area.
The experiments were performed by Dr. Till Acker and Prof. K.H. Plate, Neurological Institute, Neuropathology, Deutschordenstr. 45, 60528 Frankfurt, Germany
-
Western blot - Anti-VEGFA antibody (ab51745)All lanes : Anti-VEGFA antibody (ab51745)
Lane 1 : recombinant mouse VEGF164
Lane 2 : Recombinant human VEGF165
Lane 3 : Recombinant human PlGF-1
Lane 4 : Recombinant rat VEGF-C
Predicted band size: 27 kDa
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (43)
ab51745 has been referenced in 43 publications.
- Mouton AJ et al. Fibroblast polarization over the myocardial infarction time continuum shifts roles from inflammation to angiogenesis. Basic Res Cardiol 114:6 (2019). PubMed: 30635789
- Zhou P et al. Clinical significance of melanoma cell adhesion molecule CD146 and VEGFA expression in epithelial ovarian cancer. Oncol Lett 17:2418-2424 (2019). PubMed: 30675307
- Xu W et al. Endothelial FAM3A positively regulates post-ischaemic angiogenesis. EBioMedicine 43:32-42 (2019). PubMed: 31000420
- Chen Z et al. USP9X deubiquitinates ALDH1A3 and maintains mesenchymal identity in glioblastoma stem cells. J Clin Invest 129:2043-2055 (2019). PubMed: 30958800
- Law TSM et al. Endometrial Vascularization Characterized by Optical Coherence Tomography and Immunohistochemistry in Women Undergoing In Vitro Fertilization-Embryo Transfer Treatment. Medicina (Kaunas) 55:N/A (2019). PubMed: 30934763