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Cardiovascular Angiogenesis Growth Factors VEGF VEGF
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Anti-VEGFA antibody (ab51745)

  • Datasheet
  • SDS
Reviews (2)Q&A (2)References (43)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGFA antibody (ab51745)
  • Western blot - Anti-VEGFA antibody (ab51745)

Key features and details

  • Rabbit polyclonal to VEGFA
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Human, Recombinant fragment
  • Isotype: IgG

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Overview

  • Product name

    Anti-VEGFA antibody
    See all VEGFA primary antibodies
  • Description

    Rabbit polyclonal to VEGFA
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IHC-P
    Mouse
    Human
    WB
    Mouse
    See all applications and species data
  • Immunogen

    Recombinant full length protein corresponding to Mouse VEGFA. Recombinant mouse VEGF164
    Database link: Q00731

  • Positive control

    • SaOS2 cells. Mouse pancreas tissue.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Lyophilized:The lyophilized IgG is stable at 4°C for at least one month and for greater than a year when kept at –20°C. When reconstituted in sterile water to a concentration of >0.5 mg/ml the antibody is stable for at least six weeks at 2-4°C.
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Angiogenesis
    • Growth Factors
    • VEGF
    • VEGF
    • Signal Transduction
    • Growth Factors/Hormones
    • VEGF
    • Cancer
    • Growth factors
    • VEGF
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia
    • Metabolism
    • Types of disease
    • Cancer

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human VEGFA protein (ab55566)
    • Recombinant mouse VEGFA protein (ab9954)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab51745 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-P
Mouse
Human
WB
Mouse
Application Abreviews Notes
WB (1)
Use a concentration of 0.1 - 1 µg/ml. Predicted molecular weight: 27 kDa. The detection limit for recombinant murine VEGF-A is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
IHC-P (1)
Use a concentration of 2 - 10 µg/ml.
Notes
WB
Use a concentration of 0.1 - 1 µg/ml. Predicted molecular weight: 27 kDa. The detection limit for recombinant murine VEGF-A is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
IHC-P
Use a concentration of 2 - 10 µg/ml.

Target

  • Function

    Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth.
  • Tissue specificity

    Isoform VEGF189, isoform VEGF165 and isoform VEGF121 are widely expressed. Isoform VEGF206 and isoform VEGF145 are not widely expressed.
  • Involvement in disease

    Defects in VEGFA are a cause of susceptibility to microvascular complications of diabetes type 1 (MVCD1) [MIM:603933]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis.
  • Sequence similarities

    Belongs to the PDGF/VEGF growth factor family.
  • Cellular localization

    Secreted. VEGF121 is acidic and freely secreted. VEGF165 is more basic, has heparin-binding properties and, although a signicant proportion remains cell-associated, most is freely secreted. VEGF189 is very basic, it is cell-associated after secretion and is bound avidly by heparin and the extracellular matrix, although it may be released as a soluble form by heparin, heparinase or plasmin.
  • Target information above from: UniProt accession P15692 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 7422 Human
    • Entrez Gene: 22339 Mouse
    • Omim: 192240 Human
    • SwissProt: P15692 Human
    • SwissProt: Q00731 Mouse
    • Unigene: 73793 Human
    • Unigene: 282184 Mouse
    • Alternative names

      • Folliculostellate cell-derived growth factor antibody
      • Glioma-derived endothelial cell mitogen antibody
      • MGC70609 antibody
      • MVCD1 antibody
      • Vascular endothelial growth factor A antibody
      • vascular endothelial growth factor A121 antibody
      • vascular endothelial growth factor A165 antibody
      • vascular endothelial growth factor antibody
      • Vascular permeability factor antibody
      • VEGF A antibody
      • Vegf antibody
      • VEGF-A antibody
      • VEGF120 antibody
      • Vegfa antibody
      • VEGFA_HUMAN antibody
      • VPF antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGFA antibody (ab51745)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGFA antibody (ab51745)

      Staining of VEGF-A in paraffin-embedded mouse ventricular zone of the CNS (E17) and a perinecrotic area of a human glioblastom.

      Top panel: Mouse E17: ventricular zone of the CNS.

      Bottom panel: Human glioblastoma: perinecrotic area.

      The experiments were performed by Dr. Till Acker and Prof. K.H. Plate, Neurological Institute, Neuropathology, Deutschordenstr. 45, 60528 Frankfurt, Germany

    • Western blot - Anti-VEGFA antibody (ab51745)
      Western blot - Anti-VEGFA antibody (ab51745)
      All lanes : Anti-VEGFA antibody (ab51745)

      Lane 1 : recombinant mouse VEGF164
      Lane 2 : Recombinant human VEGF165
      Lane 3 : Recombinant human PlGF-1
      Lane 4 : Recombinant rat VEGF-C

      Predicted band size: 27 kDa

    Protocols

    • Western blot protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (43)

    Publishing research using ab51745? Please let us know so that we can cite the reference in this datasheet.

    ab51745 has been referenced in 43 publications.

    • Mouton AJ  et al. Fibroblast polarization over the myocardial infarction time continuum shifts roles from inflammation to angiogenesis. Basic Res Cardiol 114:6 (2019). PubMed: 30635789
    • Zhou P  et al. Clinical significance of melanoma cell adhesion molecule CD146 and VEGFA expression in epithelial ovarian cancer. Oncol Lett 17:2418-2424 (2019). PubMed: 30675307
    • Xu W  et al. Endothelial FAM3A positively regulates post-ischaemic angiogenesis. EBioMedicine 43:32-42 (2019). PubMed: 31000420
    • Chen Z  et al. USP9X deubiquitinates ALDH1A3 and maintains mesenchymal identity in glioblastoma stem cells. J Clin Invest 129:2043-2055 (2019). PubMed: 30958800
    • Law TSM  et al. Endometrial Vascularization Characterized by Optical Coherence Tomography and Immunohistochemistry in Women Undergoing In Vitro Fertilization-Embryo Transfer Treatment. Medicina (Kaunas) 55:N/A (2019). PubMed: 30934763
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-4 of 4 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-VEGFA antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Tumor tissue)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris EDTA pH8.0
    Permeabilization
    No
    Specification
    Tumor tissue
    Blocking step
    Serum as blocking agent for 15 minute(s) · Concentration: 50µg/mL · Temperature: 37°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Apr 23 2015

    Question


    I am interested in products ab9546 (anti-VEGFC) and ab51745 (anti-VEGFA). I am interested in specifically measuring the expression of both VEGFA and VEGFC. Since these are both rabbit polyclonal antibodies, do you know to what extent product ab9546 (anti-VEGFC) will recognize VEGFA and to what extent product ab51745 (anti-VEGFA) will recognize VEGFC?
    I am also interested in product ab105219 (anti-VEGFA). Do you know to what extent this product will recognize VEGFC?

    Read More

    Abcam community

    Verified customer

    Asked on Feb 01 2013

    Answer

    Thank you for contacting us.


    The rabbit anti-mouse VEGF-A antibody ab51745 did not cross react with human VEGF-C in Western blotting (reduced, non-reduced). According to our results the rabbit anti-rat VEGF-C ab9546 cross reacts with human VEGF-C but not with human VEGF165.

    Regarding ab105219 I am sorry, we have not tested this antibody for reactivity with VEGFC.


    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    Answered on Feb 01 2013

    Western blot abreview for Anti-VEGFA antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Melanoma cancer)
    Loading amount
    30 µg
    Specification
    Melanoma cancer
    Gel Running Conditions
    Non-reduced Denaturing (10%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Read More

    Ms. Seontae Kim

    Verified customer

    Submitted Jan 02 2013

    Question

    I have a question about ab51745 This antibody's immunogen is Recombinant murine VEGF164 Dimer. But predicted size is 27KDa. This size is monomer. Our user wants to detect just monomer. Could you confirm which one is detecting?

    Read More

    Abcam community

    Verified customer

    Asked on Jul 25 2008

    Answer

    Thank you for your enquiry. The MW of recombinant mouse VEGF164 dimer produced in insect cells is about 48 kDa, but the monomer has a MW of about 24 kDa.. The predicted size of 27 kDa is probably those of VEGF164 produced in mammalian cells. This is because insect cells have a different glycosylation than mammalian cells. The glycosylation in insect cells is lower than those of mammalian cells. If your customer wants to detect the monomer, he has to run the SDS-PAGE under reducing conditions. I hope this information will be helpful. If there is anything else that I can help you with, please do not hesitate to contact me.

    Read More

    Abcam Scientific Support

    Answered on Jul 25 2008

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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