Anti-VEGFA antibody (ab51745)
- Datasheet
- References (31)
- Protocols
Overview
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Product nameAnti-VEGFA antibody
See all VEGFA primary antibodies -
DescriptionRabbit polyclonal to VEGFA
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Host speciesRabbit
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Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
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Species reactivityReacts with: Mouse, Human
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Immunogen
Recombinant full length protein corresponding to Mouse VEGFA. Recombinant mouse VEGF164
Database link: Q00731 -
Positive control
- SaOS2 cells. Human pancreas tissue.
Properties
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FormLyophilised:The lyophilized IgG is stable at 4°C for at least one month and for greater than a year when kept at –20°C. When reconstituted in sterile water to a concentration of >0.5 mg/ml the antibody is stable for at least six weeks at 2-4°C.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: None
Constituents: PBS, pH 7.4 -
Concentration information loading... -
PurityProtein A purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab51745 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 0.1 - 1 µg/ml. Predicted molecular weight: 27 kDa. The detection limit for recombinant murine VEGF-A is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. | |
| ELISA | Use a concentration of 1 - 10 µg/ml. | |
| IHC-P | Use a concentration of 2 - 10 µg/ml. |
Target
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FunctionGrowth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth.
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Tissue specificityIsoform VEGF189, isoform VEGF165 and isoform VEGF121 are widely expressed. Isoform VEGF206 and isoform VEGF145 are not widely expressed.
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Involvement in diseaseDefects in VEGFA are a cause of susceptibility to microvascular complications of diabetes type 1 (MVCD1) [MIM:603933]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis.
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Sequence similaritiesBelongs to the PDGF/VEGF growth factor family.
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Cellular localizationSecreted. VEGF121 is acidic and freely secreted. VEGF165 is more basic, has heparin-binding properties and, although a signicant proportion remains cell-associated, most is freely secreted. VEGF189 is very basic, it is cell-associated after secretion and is bound avidly by heparin and the extracellular matrix, although it may be released as a soluble form by heparin, heparinase or plasmin.
- Information by UniProt
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Database links
- Entrez Gene: 7422 Human
- Entrez Gene: 22339 Mouse
- Omim: 192240 Human
- SwissProt: P15692 Human
- SwissProt: Q00731 Mouse
- Unigene: 73793 Human
- Unigene: 282184 Mouse
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Alternative names
- Folliculostellate cell-derived growth factor antibody
- Glioma-derived endothelial cell mitogen antibody
- MGC70609 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGFA antibody (ab51745)Staining of VEGF-A in paraffin-embedded mouse ventricular zone of the CNS (E17) and a perinecrotic area of a human glioblastom.
Top panel: Mouse E17: ventricular zone of the CNS.
Bottom panel: Human glioblastoma: perinecrotic area.
The experiments were performed by Dr. Till Acker and Prof. K.H. Plate, Neurological Institute, Neuropathology, Deutschordenstr. 45, 60528 Frankfurt, Germany
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Western blot - Anti-VEGFA antibody (ab51745)All lanes : Anti-VEGFA antibody (ab51745)
Lane 1 : recombinant mouse VEGF164
Lane 2 : Recombinant human VEGF165
Lane 3 : Recombinant human PlGF-1
Lane 4 : Recombinant rat VEGF-C
Predicted band size: 27 kDa
References
This product has been referenced in:
- Lu T et al. Blockade of ONECUT2 expression in ovarian cancer inhibited tumor cell proliferation, migration, invasion and angiogenesis. Cancer Sci 109:2221-2234 (2018). Read more (PubMed: 29737581) »
- Lun W et al. MiR-218 regulates epithelial-mesenchymal transition and angiogenesis in colorectal cancer via targeting CTGF. Cancer Cell Int 18:83 (2018). Read more (PubMed: 29977158) »
