Overview

  • Product name
    Anti-VEGFA antibody
    See all VEGFA primary antibodies
  • Description
    Rabbit polyclonal to VEGFA
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant full length protein corresponding to Mouse VEGFA. Recombinant mouse VEGF164
    Database link: Q00731

  • Positive control
    • SaOS2 cells. Human pancreas tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab51745 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 1 µg/ml. Predicted molecular weight: 27 kDa. The detection limit for recombinant murine VEGF-A is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
ELISA Use a concentration of 1 - 10 µg/ml.
IHC-P Use a concentration of 2 - 10 µg/ml.

Target

  • Function
    Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth.
  • Tissue specificity
    Isoform VEGF189, isoform VEGF165 and isoform VEGF121 are widely expressed. Isoform VEGF206 and isoform VEGF145 are not widely expressed.
  • Involvement in disease
    Defects in VEGFA are a cause of susceptibility to microvascular complications of diabetes type 1 (MVCD1) [MIM:603933]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis.
  • Sequence similarities
    Belongs to the PDGF/VEGF growth factor family.
  • Cellular localization
    Secreted. VEGF121 is acidic and freely secreted. VEGF165 is more basic, has heparin-binding properties and, although a signicant proportion remains cell-associated, most is freely secreted. VEGF189 is very basic, it is cell-associated after secretion and is bound avidly by heparin and the extracellular matrix, although it may be released as a soluble form by heparin, heparinase or plasmin.
  • Information by UniProt
  • Database links
    • Alternative names
      • Folliculostellate cell-derived growth factor antibody
      • Glioma-derived endothelial cell mitogen antibody
      • MGC70609 antibody
      • MVCD1 antibody
      • Vascular endothelial growth factor A antibody
      • vascular endothelial growth factor A121 antibody
      • vascular endothelial growth factor A165 antibody
      • vascular endothelial growth factor antibody
      • Vascular permeability factor antibody
      • VEGF A antibody
      • Vegf antibody
      • VEGF-A antibody
      • VEGF120 antibody
      • Vegfa antibody
      • VEGFA_HUMAN antibody
      • VPF antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGFA antibody (ab51745)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGFA antibody (ab51745)

      Staining of VEGF-A in paraffin-embedded mouse ventricular zone of the CNS (E17) and a perinecrotic area of a human glioblastom.

      Top panel: Mouse E17: ventricular zone of the CNS.

      Bottom panel: Human glioblastoma: perinecrotic area.

      The experiments were performed by Dr. Till Acker and Prof. K.H. Plate, Neurological Institute, Neuropathology, Deutschordenstr. 45, 60528 Frankfurt, Germany

    • Western blot - Anti-VEGFA antibody (ab51745)
      Western blot - Anti-VEGFA antibody (ab51745)
      All lanes : Anti-VEGFA antibody (ab51745)

      Lane 1 : recombinant mouse VEGF164
      Lane 2 : Recombinant human VEGF165
      Lane 3 : Recombinant human PlGF-1
      Lane 4 : Recombinant rat VEGF-C

      Predicted band size: 27 kDa

    References

    This product has been referenced in:
    • Li Z  et al. Ferulic Acid Improves Functional Recovery after Acute Spinal Cord Injury in Rats by Inducing Hypoxia to Inhibit microRNA-590 and Elevate Vascular Endothelial Growth Factor Expressions. Front Mol Neurosci 10:183 (2017). WB ; Rat . Read more (PubMed: 28642684) »
    • Yang X  et al. DPPIV promotes endometrial carcinoma cell proliferation, invasion and tumorigenesis. Oncotarget 8:8679-8692 (2017). WB . Read more (PubMed: 28060721) »

    See all 20 Publications for this product

    Publishing research using ab51745? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    4 reviews or Q&A

    10
    Votes: Highest First

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-VEGFA antibody

     Excellent
    Abreviews
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Tumor tissue)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris EDTA pH8.0
    Permeabilization
    No
    Specification
    Tumor tissue
    Blocking step
    Serum as blocking agent for 15 minute(s) · Concentration: 50µg/mL · Temperature: 37°C
    Fixative
    Paraformaldehyde
    Read More
    abreview image
    Username

    Abcam user community

    Verified customer

    Submitted Apr 23 2015


    I am interested in products ab9546 (anti-VEGFC) and ab51745 (anti-VEGFA). I am interested in specifically measuring the expression of both VEGFA and VEGFC. Since these are both rabbit polyclonal antibodies, do you know to what extent product ab954...

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    Thank you for contacting us.


    The rabbit anti-mouse VEGF-A antibody ab51745 did not cross react with human VEGF-C in Western blotting (reduced, non-reduced). According to our results the rabbit anti-rat VEGF-C ab9546 cross reacts with huma...

    Read More

    Western blot abreview for Anti-VEGFA antibody

     Excellent
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Melanoma cancer)
    Loading amount
    30 µg
    Specification
    Melanoma cancer
    Gel Running Conditions
    Non-reduced Denaturing (10%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Read More
    abreview image
    Username

    Ms. Seontae Kim

    Verified customer

    Submitted Jan 02 2013

    I have a question about ab51745 This antibody's immunogen is Recombinant murine VEGF164 Dimer. But predicted size is 27KDa. This size is monomer. Our user wants to detect just monomer. Could you confirm which one is detecting?

    Thank you for your enquiry. The MW of recombinant mouse VEGF164 dimer produced in insect cells is about 48 kDa, but the monomer has a MW of about 24 kDa.. The predicted size of 27 kDa is probably those of VEGF164 produced in mammalian cells. This i...

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