Product nameAnti-VEGFA antibody (Biotin)
See all VEGFA primary antibodies
DescriptionRabbit polyclonal to VEGFA (Biotin)
Tested applicationsSuitable for: WB, Sandwich ELISAmore details
Species reactivityReacts with: Human
Recombinant full length protein corresponding to Human VEGFA. Expressed in E. coli.
Database link: P15692
- WB: HUVEC, MCF7, A549, HAEC and HeLa whole cell extracts.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark.
Storage bufferConstituents: PBS, 4% BSA
Concentration information loading...
PurityProtein A/G purified
Our Abpromise guarantee covers the use of ab267566 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 27 kDa.|
|Sandwich ELISA||Use a concentration of 0.125 - 0.5 µg/ml.
Typical dilutions for sandwich ELISA include 1 μg/mL for coating and 0.125-0.5 μg/mL for detection.
Can be paired for Sandwich ELISA with Rabbit polyclonal to VEGFA (Biotin) (ab83132). To detect hVEGF by sandwich ELISA (using 100 µl/well antibody solution) a concentration of 0.5 - 2.0 µg/ml of this antibody as Detection is required. This antigen affinity purified antibody, paired with the above recommended pair, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hVEGF (ab9571).
FunctionGrowth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth.
Tissue specificityIsoform VEGF189, isoform VEGF165 and isoform VEGF121 are widely expressed. Isoform VEGF206 and isoform VEGF145 are not widely expressed.
Involvement in diseaseDefects in VEGFA are a cause of susceptibility to microvascular complications of diabetes type 1 (MVCD1) [MIM:603933]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis.
Sequence similaritiesBelongs to the PDGF/VEGF growth factor family.
Cellular localizationSecreted. VEGF121 is acidic and freely secreted. VEGF165 is more basic, has heparin-binding properties and, although a signicant proportion remains cell-associated, most is freely secreted. VEGF189 is very basic, it is cell-associated after secretion and is bound avidly by heparin and the extracellular matrix, although it may be released as a soluble form by heparin, heparinase or plasmin.
- Information by UniProt
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All lanes : Anti-VEGFA antibody (Biotin) (ab267566) at 1 µg/ml
Lane 1 : HUVEC (human umbilical vein endothelial cell line) whole cell extract
Lane 2 : MCF7 (human breast adenocarcinoma cell line) whole cell extract
Lane 3 : A549 (human lung carcinoma cell line) whole cell extract
Lane 4 : HAEC whole cell extract
Lane 5 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 27 kDa
The plate was coated with 100 μL ab155944 at 1, 3, 4, 5 7 and 9 μg/mL in DPBS for 12 - 18 hours at 4°C. After washing the plate was blocked with 300 μL per well of 4% BSA / 5% sucrose in DPBS for 1 hour at room temperature.
Recombinant human VEGF protein (50 μL /well) was added at 2000, 1000, 500, 250, 125, 62.5, 31.25, and 0 pg/mL for 2 hours at room temperature.
After washing the plate was incubated with ab267566 at 0.5 μg /Ml for 1 hour at room temperature.
Detection was with Streptavidin-HRP / TMB.
ab267566 has not yet been referenced specifically in any publications.