Overview

  • Product name
    Anti-VEGFA antibody (Biotin)
    See all VEGFA primary antibodies
  • Description
    Rabbit polyclonal to VEGFA (Biotin)
  • Host species
    Rabbit
  • Conjugation
    Biotin
  • Tested applications
    Suitable for: Sandwich ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein corresponding to Human VEGFA.

Properties

Applications

Our Abpromise guarantee covers the use of ab83132 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use a concentration of 0.25 - 1 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to VEGFA (ab9570). To detect hVEGF by sandwich ELISA (using 100 µl/well antibody solution) a concentration of 0.25 - 1.0 µg/ml of this antibody as Capture is required. This biotinylated polyclonal antibody, paired with the above recommended pair, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hVEGF.
WB Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 27 kDa. The detection limit for recombinant human VEGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Target

  • Function
    Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth.
  • Tissue specificity
    Isoform VEGF189, isoform VEGF165 and isoform VEGF121 are widely expressed. Isoform VEGF206 and isoform VEGF145 are not widely expressed.
  • Involvement in disease
    Defects in VEGFA are a cause of susceptibility to microvascular complications of diabetes type 1 (MVCD1) [MIM:603933]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis.
  • Sequence similarities
    Belongs to the PDGF/VEGF growth factor family.
  • Cellular localization
    Secreted. VEGF121 is acidic and freely secreted. VEGF165 is more basic, has heparin-binding properties and, although a signicant proportion remains cell-associated, most is freely secreted. VEGF189 is very basic, it is cell-associated after secretion and is bound avidly by heparin and the extracellular matrix, although it may be released as a soluble form by heparin, heparinase or plasmin.
  • Information by UniProt
  • Database links
  • Alternative names
    • Folliculostellate cell-derived growth factor antibody
    • Glioma-derived endothelial cell mitogen antibody
    • MGC70609 antibody
    • MVCD1 antibody
    • Vascular endothelial growth factor A antibody
    • vascular endothelial growth factor A121 antibody
    • vascular endothelial growth factor A165 antibody
    • vascular endothelial growth factor antibody
    • Vascular permeability factor antibody
    • VEGF A antibody
    • Vegf antibody
    • VEGF-A antibody
    • VEGF120 antibody
    • Vegfa antibody
    • VEGFA_HUMAN antibody
    • VPF antibody
    see all

Images

  • Detected human VEGF in a sandwich ELISA within the range 16-1000 pg/ml
    - 0.5 µg/ml of capture antibody (ab9570)
    - 0.25 µg/ml of detection antibody (ab83132)

References

ab83132 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-6 of 6 Abreviews or Q&A

Answer

Thank you for contacting us.

The antibodies are viable for 10 years when stored at -20C, for 1 year when stored at 4C.

Please note that we recommend storing the antibodies at 4C for 1-2 weeks only. Some antibodies are prone to degradation if stored longer than a month so please check the datasheet for storage conditions.

ab106041; Store at -20°C, Aliquot to avoid repeated freezing and thawing. will be viable for 10 years
ab106310; Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. will be viable for 10 years at -20 or -80C.
ab51516; Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles; viable for 10 years
ab54346; Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles; viable for 10 years at -20C.
ab54347; Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles; viable for 10 years at -20C
ab83132; Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles; viable for 10 years at -20C

Click the link for more up-to-date information:

https://www.abcam.com/index.html?pageconfig=resource&rid=10795

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Question
Answer

Merci de votre intérêt pour ab83132 et ab93540.
https://www.abcam.com/ab83132
https://www.abcam.com/ab93540

Comme expliqué par téléphone, à notre connaissance ces deuxanticorps n’ont pas encore été testés en IHC-P et nous n’offrons pas d’échantillon gratuit à des fins de tests préliminaires. Cependant, si vous souhaitez tester ab83132 et ab93540en IHC-P, je peux vous proposer notre offre de test. Après soumission d’une Abreview avec vos résultats, un anticorps primaire vous sera offert pour chaque résultat partagé.

Etapes à suivre :

1. Nous confirmer que vous souhaitez tester ab83132 et/ou ab93540 en IHC-P afin de recevoir les codes de remise correspondant. Important, ce code doit être édité avant l’achat de ab83132 et ab93540

2. Acheter ab83132 et ab93540 par téléphone, fax ou internet (www.abcam.com)

3. Les tester en IHC-P

4. Nous faire part de vos résultats, positifs ou négatifs, grâce à notre système Abreview. Pour plus d’informations https://www.abcam.com/abreviews

5. Après soumission de votre Abreview, appeler notre service clientèle afin de passer votre prochaine commande en utilisant le code de réduction. Ce code de réduction est utilisable 120 jours après son édition et utilisable pour la commande d’un autre anticorps primaire.
Même si ab83132 ou ab93540 ne fonctionne pas en IHC-P, après soumission de votre Abreview, vous recevrez quand même un anticorps primaire gratuit.

N’hésitez pas à nous demander plus d’informations concernant cette offre promotionnelle.

Termes et Conditions : https://www.abcam.com/collaborationdiscount .

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Question
Answer

Thank you for contacting us.

Thefollowing CROSS REACTIVITY has been tested with the antibodies ab9570 and ab83132:

When prepared at 50ng/ml the following antigens exhibited 100% cross reactivity:

Human VEGF121
Murine VEGF
Rat VEGF

When prepared at 50ng/ml the following antigens did NOT exhibit significant cross reactivity:

Human EGF, EG-VEGF, FGF-16, GM-CSF, PDGF-AA, PDGF-AB, PDGF-BB, PDGF-CC, RANTES, SCF, VEGF-B, VEGF-C, VEGF-D
Murine EGF, GM-CSF, PDGF-AA, PDGF-BB, SCF
Rat EGF, SCF

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Answer

Thank you for contacting us.

We do actually recommend storing the lyophilised antibody at -20C for long term storage; this will be stable for 2 years.

The antibody should be stored lyophilised at -20C till use and then reconstituted and stored per the datasheet.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Answer

Thank you for your reply.

Below please find the protocol information you requested:

SOLUTIONS
All solutions should be at ambient temperature prior to use.
PBS: Dilute 10xPBS to 1xPBS, pH 7.20 in sterile water.
Wash Buffer: 0.05% Tween-20 in PBS
Block Buffer: 1% BSA in PBS*
Diluent: 0.05% Tween-20, 0.1% BSA in PBS*
*Sterile filter and store at 4°C for up to 1 week.


PLATE PREPARATION


- Dilute capture antibody with PBS to a concentration of 1μg/ml. Immediately, add 100ul to each ELISA plate well. Seal the plate and incubate overnight at room temperature.
- Aspirate the wells to remove liquid and wash plates 4 times. Each wash consists of adding 300ul wash buffer per well, followed by aspiration. After the last wash invert plate to remove residual buffer and blot on paper towel.
- Add 300ul blocking buffer to each well. Incubate 1 hour at R.T.
- Aspirate and wash plate 4 times (as in step 2).




ELISA PROTOCOL
Standard/Sample: Serial dilute standard from 0.01ug/ml to zero in diluent. Add 100ul of standard or sample to each well in triplicate. Incubate at room temperature for at least 2 hours.

Detection: Wash plate four times. Dilute detection antibody (biotinylated) in diluent to a concentration of 0.5 - 1.0 ug/ml. Immediately add 100 ul per well. Incubate at room temperature for 2 hours.

Avidin-HRP Conjugate: Aspirate and wash plate 4 times. Dilute Avidin-HRP conjugate 1:2000 in diluent.
Add 100 uper well. Incubate 30 min at room temperature.

ABTS Liquid Substrate: Aspirate and wash plate 4 times. Add 100ul of substrate solution to each well.
Incubate at room temperature for color development. Monitor color development with an ELISA plate reader at 405 nm with wavelength correction set at 650 nm.

NOTE: Reliable standard curves are obtained when O.D. readings do not exceed 0.2 units for the zero standard concentrations, or 1.2 units for the highest standard concentration. The plate should be monitored at 5 minute intervals until desired O.D. readings are obtained. The typical range is 5-40 minutes. O.D. readings may vary.



I hope this information is helpful. Please do not hesitate to contact us if you have any additional questions.

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Question
Answer

Thank you for contacting Abcam regarding the sELISA protocol using ab83132 and ab9570.

I have confirmed with the laboratory that a streptavidn-peroxidase was used for detection. The catalog number for this product is ab64269.

I hope this information is helpful. Please do not hesitate to contact us if you have any additional questions.

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