Application
Western blot
Sample
Mouse Recombinant protein (supernatant from LLC cell culture)
Gel Running Conditions
Reduced Denaturing (4-12% Bolt)
Loading amount
15 µg
Specification
supernatant from LLC cell culture
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/600
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: Anti-rabbit IgG, HRP-linked Antibody #7074
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
3 minute(s) and 0 second(s)
Bands
Specific: 22-25 kDa Non-specific: 35 kDa
Positive control
1st lane: Recombinant Human VEGF 165 Protein
Additional data
Additional Notes
1st lane: Recombinant Human VEGF 165 Protein
2nd lane: supernatant from LLC cells
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Haojie Fu
Verified customer
Submitted Aug 03 2021