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I would like to test this antibody for VEGF expression in mammalian cell lysates using Western blot analysis. As a positive control, I would like to include some recombinant VEGF protein in my SDS-PAGE. Please could you tell me what recombinant protein (5 ng) you used in the WB shown in the data sheet I received with this antibody.
Asked on Oct 25 2012
The recombinant protein that is the sample in the western blot on the datasheet is not commercially available. We do, however, have a similar protein, ab9571, which is a dimer of two 165 amino acid chains which contain the amino acid sequence of the ab52917 immunogen, and so will be recognized by ab52917.
Click here (or use the following: https://www.abcam.com/index.html?datasheet=9571).
Reduced samples will appear at about 22 kDa, similar to the western blot shown on the datasheet for mouse VEGF, ab62134.
Click here (or use the following: https://www.abcam.com/index.html?datasheet=62134).
Answered on Oct 25 2012