Our Abpromise guarantee covers the use of ab119 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 38-44 kDa.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol. use Tris HCl (pH 10) and NOT citrate buffer.


  • Function
    Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth.
  • Tissue specificity
    Isoform VEGF189, isoform VEGF165 and isoform VEGF121 are widely expressed. Isoform VEGF206 and isoform VEGF145 are not widely expressed.
  • Involvement in disease
    Defects in VEGFA are a cause of susceptibility to microvascular complications of diabetes type 1 (MVCD1) [MIM:603933]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis.
  • Sequence similarities
    Belongs to the PDGF/VEGF growth factor family.
  • Cellular localization
    Secreted. VEGF121 is acidic and freely secreted. VEGF165 is more basic, has heparin-binding properties and, although a signicant proportion remains cell-associated, most is freely secreted. VEGF189 is very basic, it is cell-associated after secretion and is bound avidly by heparin and the extracellular matrix, although it may be released as a soluble form by heparin, heparinase or plasmin.
  • Information by UniProt
  • Database links
  • Alternative names
    • Folliculostellate cell-derived growth factor antibody
    • Glioma-derived endothelial cell mitogen antibody
    • MGC70609 antibody
    • MVCD1 antibody
    • Vascular endothelial growth factor A antibody
    • vascular endothelial growth factor A121 antibody
    • vascular endothelial growth factor A165 antibody
    • vascular endothelial growth factor antibody
    • Vascular permeability factor antibody
    • VEGF A antibody
    • Vegf antibody
    • VEGF-A antibody
    • VEGF120 antibody
    • Vegfa antibody
    • VEGFA_HUMAN antibody
    • VPF antibody
    see all


This product has been referenced in:
  • Adeoye OO  et al. VEGF receptors mediate hypoxic remodeling of adult ovine carotid arteries. J Appl Physiol (1985) 117:777-87 (2014). Read more (PubMed: 25038104) »
  • Adeoye OO  et al. Contribution of increased VEGF receptors to hypoxic changes in fetal ovine carotid artery contractile proteins. Am J Physiol Cell Physiol 304:C656-65 (2013). Read more (PubMed: 23325408) »

See all 5 Publications for this product

Customer reviews and Q&As

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ab2185 Immunogen sequence is more than 90% similar with Pig HIF1 alpha so this antibody is more likely to cross react.


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Thank you for getting back in touch with me. Im afraid that I have provided you with all of the information that I have with regards this antibody. Given the information that you have obtained it is highly plausible that the non-reducing conditions are...

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Thank you for getting back to me. I am currently in touch with the lab to determine whether they have any further information as to the analysis of VEGF using a non-reducing gel. Yes, the omission of beta-mercaptoethanol would be sufficient for thi...

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Thank you for getting back to me. That is certainly a possibility. The clones should be identical aside from individual laboratory preparation differences. Please can you tell me where you obtained this information for this clone, I am most interes...

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Thank you for your enquiry. To our knowledge this product will cross react with VEGF165 ( J Pathol, 185: 402-408 (1998). We do not have any information regarding the product's cross reactively with other isoforms of VEGF. If you need further informatio...

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Thanks for your email. I'm replying to you on behalf of Chris, who is currently away on vacation. To use ab119 in IHC, I would recommend trying a range of dilutions in order to determine which is optimal for your particular experiment: 1:50, 1:100, 1:2...

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Thank you for your previous enquiry regarding the conservation of VEGF. I have been awaiting clarification of the immunogen used. The immunogen used to make the monoclonal was: the whole VEGF protein (subtype 189). The epitope that this product recogni...

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This goes along with the other email that I just sent you. First, ab11844 has only been tested for application in IHC. The WB listing on the datasheet was a mistake and has since been updated - I do apologize for this. Second, do you want a refund for ...

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Thank you for your earlier enquiry. This product will cross react with VEGF165 ( J Pathol, 185: 402-408 (1998). We do not have any information regarding the product's cross reactively with other isoforms of VEGF. If you need any further assistance plea...

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Thank you for your email. I'm sorry to hear that ab119 did not work out for you and I can issue you a refund for this antibody - please send me the Abcam order number or purchase order number that was used when you ordered ab119. For the HIF1 alpha ...

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1-10 of 26 Abreviews or Q&A


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