Overview

  • Product name
  • Description
    Rabbit polyclonal to VEGFC
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human VEGFC.
    Database link: P49767

  • Positive control
    • WB: 293, CCRF-CEM and K562 cell lysates. FC: 293 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab135506 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/2000. Predicted molecular weight: 46 kDa.
Flow Cyt 1/10 - 1/50. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Function
    Growth factor active in angiogenesis, and endothelial cell growth, stimulating their proliferation and migration and also has effects on the permeability of blood vessels. May function in angiogenesis of the venous and lymphatic vascular systems during embryogenesis, and also in the maintenance of differentiated lymphatic endothelium in adults. Binds and activates VEGFR-2 (KDR/FLK1) and VEGFR-3 (FLT4) receptors.
  • Tissue specificity
    Spleen, lymph node, thymus, appendix, bone marrow, heart, placenta, ovary, skeletal muscle, prostate, testis, colon and small intestine and fetal liver, lung and kidney, but not in peripheral blood lymphocyte.
  • Sequence similarities
    Belongs to the PDGF/VEGF growth factor family.
  • Post-translational
    modifications
    Undergoes a complex proteolytic maturation which generates a variety of processed secreted forms with increased activity toward VEGFR-3, but only the fully processed form could activate VEGFR-2. VEGF-C first form an antiparallel homodimer linked by disulfide bonds. Before secretion, a cleavage occurs between Arg-227 and Ser-228 producing an heterotetramer. The next extracellular step of the processing removes the N-terminal propeptide. Finally the mature VEGF-C is composed mostly of two VEGF homology domains (VHDs) bound by non-covalent interactions.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Flt 4L antibody
    • Flt4 ligand antibody
    • FLT4 ligand DHM antibody
    • Flt4-L antibody
    • Flt4L antibody
    • Vascular endothelial growth factor C antibody
    • Vascular endothelial growth factor related protein antibody
    • Vascular endothelial growth factor-related protein antibody
    • VEGF C antibody
    • VEGF-C antibody
    • Vegfc antibody
    • VEGFC_HUMAN antibody
    • VRP antibody
    see all

Images

  • All lanes : Anti-VEGFC antibody (ab135506) at 1/2000 dilution

    Lane 1 : 293 whole cell lysate
    Lane 2 : CCRF-CEM whole cell lysate
    Lane 3 : K562 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Peroxidase conjugated goat anti-rabbit IgG (H+L) at 1/10000 dilution

    Predicted band size: 46 kDa
    Observed band size: 47 kDa
    why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Flow cytometry analysis of HepG2 cells labelling VEGFC with ab135506 (green). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. Samples were blocked with 2% BSA followed by incubation with the antibody at a dilution of 1/25 for 60 min at 37°C. A DyLight®-488 conjugated goat anti-rabbit IgG was used as the secondary antibody at 1/200 dilution for 40 min at 37°C. As an isotype control (blue) rabbit IgG1 (1μg/1x106 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
     

References

This product has been referenced in:
  • Vesci L  et al. Therapeutic efficacy of intra-tumor AvidinOX and low systemic dose biotinylated cetuximab, with and without cisplatin, in an orthotopic model of head and neck cancer. Oncol Lett 17:3529-3536 (2019). Read more (PubMed: 30867794) »
  • Huang Q  et al. Androgen receptor increases hematogenous metastasis yet decreases lymphatic metastasis of renal cell carcinoma. Nat Commun 8:918 (2017). Read more (PubMed: 29030639) »
See all 4 Publications for this product

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