Overview

  • Product name

  • Description

    Rabbit polyclonal to VEGFD
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Macaque monkey
  • Immunogen

    Synthetic peptide corresponding to Human VEGFD aa 300 to the C-terminus conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab119174)

  • Positive control

    • This antibody gave a positive signal in HT1080 whole cell lysate as well as the following tissue lysates: Human Heart; Human Fetal Lung; Human Colon.

Properties

Applications

Our Abpromise guarantee covers the use of ab103685 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).
IHC-P Use a concentration of 10 µg/ml.

Target

  • Function

    Growth factor active in angiogenesis, lymphangiogenesis and endothelial cell growth, stimulating their proliferation and migration and also has effects on the permeability of blood vessels. May function in the formation of the venous and lymphatic vascular systems during embryogenesis, and also in the maintenance of differentiated lymphatic endothelium in adults. Binds and activates VEGFR-2 (KDR/FLK1) and VEGFR-3 (FLT4) receptors.
  • Tissue specificity

    Highly expressed in lung, heart, small intestine and fetal lung, and at lower levels in skeletal muscle, colon, and pancreas.
  • Sequence similarities

    Belongs to the PDGF/VEGF growth factor family.
  • Post-translational
    modifications

    Undergoes a complex proteolytic maturation which generates a variety of processed secreted forms with increased activity toward VEGFR-3 and VEGFR-2. VEGF-D first form an antiparallel homodimer linked by disulfide bonds before secretion. The fully processed VEGF-D is composed mostly of two VEGF homology domains (VHDs) bound by non-covalent interactions.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • c-fos induced growth factor antibody
    • c-fos induced growth factor (vascular endothelial growth factor D) antibody
    • c-fos induced growth factor antibody
    • c-fos-induced growth factor antibody
    • FIGF antibody
    • Vascular endothelial growth factor D antibody
    • Vascular endothelial growth factor D precursor antibody
    • Vascular endothelial growth factor D precursor antibody
    • VEGF D antibody
    • VEGF-D antibody
    • VEGFD antibody
    • VEGFD_HUMAN antibody
    see all

Images

  • All lanes : Anti-VEGFD antibody (ab103685) at 1 µg/ml

    Lane 1 : HT 1080 (Human fibrosarcoma) Whole Cell Lysate
    Lane 2 : Human heart tissue lysate - total protein (ab29431)
    Lane 3 : Human Fetal Heart - Total Protein Tissue Lysate
    Lane 4 : Human colon tissue lysate - total protein (ab30051)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 40 kDa
    Observed band size: 40 kDa
    Additional bands at: 140 kDa, 45 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 8 minutes


    Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
  • IHC image of VEGFD staining in Human heart muscle formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab103685, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References

This product has been referenced in:

  • Kim HJ  et al. Association Between c-Met and Lymphangiogenic Factors in Patients With Colorectal Cancer. Ann Coloproctol 34:88-93 (2018). Read more (PubMed: 29742856) »
See 1 Publication for this product

Customer reviews and Q&As

Answer

Thank you for contacting us.

Because we carry over 70,000 products, it isn't feasible for us to keep small sample sizes of our products.
We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datasheet, or we will offer a replacement, credit, or refund within 6 months of purchase.

A discount can be offered if you purchase 5 products or more.

Please find below the selection of antibodies that, I think, may be suitable for your work, assuming you work on Human tissue samples:

VEGF : https://www.abcam.com/ab46154 (Rabbit polyclonal), https://www.abcam.com/ab1316 (Mouse monoclonal) or https://www.abcam.com/ab68334 (Mouse monoclonal). All very well characterised and tested in IHC-P.
VEGFA : https://www.abcam.com/ab105219 (Mouse monoclonal). Tested in IHC-P.
VEGFB : https://www.abcam.com/ab58461(Rabbit polyclonal). Not yet tested in IHC-P. Eligible for a testing discount (see details below).
VEGFC : https://www.abcam.com/ab9546 (Rabbit polyclonal), tested in IHC-P.
VEGFD : https://www.abcam.com/ab103685 (Rabbit polyclonal), tested in IHC-P




To our knowledge, ab58461 has not been tested in IHC-P. Therefore, I can offer a discount off a future purchase if you buy ab58461 now, test it in IHC-P and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of: 1 free primary antibody.

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test ab58461 in IHC-P. I will then send a discount code. This code must be issued before purchasing ab58461 so please wait for my reply before ordering.

2. Purchase ab58461 either by phone, fax, or online (https://www.abcam.com/).

3. Test it in IHC-P.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue.
Please remember that submission of the Abreview is sufficient for the discount code to become active.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab58461 turns out to be unsuitable for IHC-P, you will still receive the discount on your next purchase after your Abreview has been submitted.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: https://www.abcam.com/collaborationdiscount.

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