Key features and details
- Rabbit polyclonal to VEGFD
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-VEGFD antibody
See all VEGFD primary antibodies
DescriptionRabbit polyclonal to VEGFD
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Recombinant fragment, corresponding to a region within amino acids 163-354 of Human VEGFD (Uniprot ID O43915).
- 293T, Raji whole cell lysates; MOLT4 cell line; Human SAS xenograft tissue.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab137368 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 40 kDa.|
|IHC-P||1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval using heat mediated Tris-EDTA buffer (pH8.0) is also possible.|
FunctionGrowth factor active in angiogenesis, lymphangiogenesis and endothelial cell growth, stimulating their proliferation and migration and also has effects on the permeability of blood vessels. May function in the formation of the venous and lymphatic vascular systems during embryogenesis, and also in the maintenance of differentiated lymphatic endothelium in adults. Binds and activates VEGFR-2 (KDR/FLK1) and VEGFR-3 (FLT4) receptors.
Tissue specificityHighly expressed in lung, heart, small intestine and fetal lung, and at lower levels in skeletal muscle, colon, and pancreas.
Sequence similaritiesBelongs to the PDGF/VEGF growth factor family.
modificationsUndergoes a complex proteolytic maturation which generates a variety of processed secreted forms with increased activity toward VEGFR-3 and VEGFR-2. VEGF-D first form an antiparallel homodimer linked by disulfide bonds before secretion. The fully processed VEGF-D is composed mostly of two VEGF homology domains (VHDs) bound by non-covalent interactions.
- Information by UniProt
- c-fos induced growth factor antibody
- c-fos induced growth factor (vascular endothelial growth factor D) antibody
- c-fos induced growth factor antibody
All lanes : Anti-VEGFD antibody (ab137368) at 1/1000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : Raji whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 40 kDa
10% SDS PAGE
Immunohistochemical analysis of paraffin-embedded Human SAS xenograft tissue labeling VEGFD, using ab137368 at a 1/100 dilution.
ab137368 has not yet been referenced specifically in any publications.