Recombinant
RabMAb

Recombinant Anti-VEGFD antibody [EPR8457] (Biotin) (ab204679)

Overview

  • Product name

    Anti-VEGFD antibody [EPR8457] (Biotin)
    See all VEGFD primary antibodies
  • Description

    Rabbit monoclonal [EPR8457] to VEGFD (Biotin)
  • Host species

    Rabbit
  • Conjugation

    Biotin
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human VEGFD. The exact sequence is proprietary.
    Database link: O43915
    (Peptide available as ab189506)

  • Positive control

    • IHC-P: Human heart muscle (normal) tissue sections.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab204679 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Growth factor active in angiogenesis, lymphangiogenesis and endothelial cell growth, stimulating their proliferation and migration and also has effects on the permeability of blood vessels. May function in the formation of the venous and lymphatic vascular systems during embryogenesis, and also in the maintenance of differentiated lymphatic endothelium in adults. Binds and activates VEGFR-2 (KDR/FLK1) and VEGFR-3 (FLT4) receptors.
  • Tissue specificity

    Highly expressed in lung, heart, small intestine and fetal lung, and at lower levels in skeletal muscle, colon, and pancreas.
  • Sequence similarities

    Belongs to the PDGF/VEGF growth factor family.
  • Post-translational
    modifications

    Undergoes a complex proteolytic maturation which generates a variety of processed secreted forms with increased activity toward VEGFR-3 and VEGFR-2. VEGF-D first form an antiparallel homodimer linked by disulfide bonds before secretion. The fully processed VEGF-D is composed mostly of two VEGF homology domains (VHDs) bound by non-covalent interactions.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • c-fos induced growth factor antibody
    • c-fos induced growth factor (vascular endothelial growth factor D) antibody
    • c-fos induced growth factor antibody
    • c-fos-induced growth factor antibody
    • FIGF antibody
    • Vascular endothelial growth factor D antibody
    • Vascular endothelial growth factor D precursor antibody
    • Vascular endothelial growth factor D precursor antibody
    • VEGF D antibody
    • VEGF-D antibody
    • VEGFD antibody
    • VEGFD_HUMAN antibody
    see all

Images

  • IHC image of VEGFD staining in a section of formalin-fixed paraffin-embedded human heart muscle*. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins, performed on a Leica BONDTM. The section was then incubated overnight at +4°C with ab204679 at 1/100 dilution. Detection was via an HRP conjugated ABC system and DAB was used as the chromogen (ab103723), diluted 1/100 and incubated for 10min at room temperature. The section was then counterstained with haematoxylin and mounted with DPX.

    The inset negative control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

References

ab204679 has not yet been referenced specifically in any publications.

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