Product nameVenlafaxine hydrochloride, Serotonin-norepinephrine reuptake inhibitor
DescriptionSerotonin-norepinephrine reuptake inhibitor
- Wy 54030
Biological descriptionSerotonin-norepinephrine reuptake inhibitor. Ki values are 82 and 2480 nM for SERT and NET, respectively. Displays 30-fold higher affinity for SERT than NET in vitro. Antidepressant.
This product is reported to be light sensitive
Chemical name1-[2-(Dimethylamino)-1-(4-methoxyphenyl)ethyl]cyclohexanol hydrochloride
Storage instructionsStore at Room Temperature. The product can be stored for up to 12 months.
Solubility overviewSoluble in water to 100 mM and in DMSO to 50 mM
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
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Our Abpromise guarantee covers the use of ab120715 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
ab18207 staining beta III tubulin in PC-12 cells treated with venlafaxine hydrochloride (ab120715), by ICC/IF. Increase in the number and length of neurites (stained with beta III tubulin) correlates with increased concentration of venlafaxine hydrochloride, as described in literature.
The NGF treated cells were incubated at 37°C for 6 hour in media containing different concentrations of ab120715 (venlafaxine hydrochloride) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab18207 (1 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody.
This product has been referenced in:
- Yilmaz N et al. Effects of venlafaxine and escitalopram treatments on NMDA receptors in the rat depression model. J Membr Biol 242:145-51 (2011). Read more (PubMed: 21755298) »
- Bymaster FP et al. Comparative affinity of duloxetine and venlafaxine for serotonin and norepinephrine transporters in vitro and in vivo, human serotonin receptor subtypes, and other neuronal receptors. Neuropsychopharmacology 25:871-80 (2001). Read more (PubMed: 11750180) »