Key features and details
- Rabbit polyclonal to VEPH1
- Suitable for: ICC, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-VEPH1 antibody
See all VEPH1 primary antibodies
DescriptionRabbit polyclonal to VEPH1
Tested applicationsSuitable for: ICC, IHC-P, WBmore details
Species reactivityReacts with: Human
antigen sequence, corresponding to amino acids 16-149 of Human VEPH1. (Q14D04)
- IHC: Human tonsil tissue; WB: VEPH1 over-expression HEK293T lysate. ICC: Human cell line A-431
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab121875 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC||Use at an assay dependent concentration.|
|IHC-P||1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.04 - 0.4 µg/ml.|
Sequence similaritiesBelongs to the MELT/VEPH family.
Contains 1 PH domain.
DomainThe PH domain is required for membrane targeting.
Cellular localizationCell membrane.
- Information by UniProt
- 2810471M23Rik antibody
- MELT antibody
- MELT_HUMAN antibody
ab121875 at 1/20 dilution staining VEPH1 in Paraffin-embedded Human Tonsil tissue by Immunohistochemistry.
All lanes : Anti-VEPH1 antibody (ab121875) at 1/250 dilution
Lane 1 : Negative control (vector only transfected HEK293T lysate)
Lane 2 : Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells
Developed using the ECL technique.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cell line A-431 labelling VEPH1 with ab121875 at 2 µg/mL or dilution
ab121875 has not yet been referenced specifically in any publications.