Overview

  • Product name
  • Description
    Rabbit polyclonal to Versican
  • Host species
    Rabbit
  • Specificity
    This antibody can be used to detect versican V1 (ADAMTS-1/4 site). This product is against the neoepitope of versican generated by cleavage of the versican core protein by ATAMTS 1/4.
  • Tested applications
    Suitable for: IHC-Fr, WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human, Pig
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide: CGG- DPEAAE, corresponding to amino acids 436-441 of Human Versican (Peptide available as ab39784.)

  • Positive control
    • WB: Human aorta lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab19345 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration. PubMed: 28955046
WB Use a concentration of 1 µg/ml. Detects a band of approximately 70 kDa.Can be blocked with Human Versican peptide (ab39784).
IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. PubMed: 18641286

Target

  • Function
    May play a role in intercellular signaling and in connecting cells with the extracellular matrix. May take part in the regulation of cell motility, growth and differentiation. Binds hyaluronic acid.
  • Tissue specificity
    Cerebral white matter and plasma. Isoform V0 and isoform V1 are expressed in normal brain, gliomas, medulloblastomas, schwannomas, neurofibromas, and meningiomas. Isoform V2 is restricted to normal brain and gliomas. Isoform V3 is found in all these tissues except medulloblastomas.
  • Involvement in disease
    Defects in VCAN are the cause of Wagner syndrome type 1 (WGN1) [MIM:143200]. WGN is a dominantly inherited vitreoretinopathy characterized by an optically empty vitreous cavity with fibrillary condensations and a preretinal avascular membrane. Other optical features include progressive chorioretinal atrophy, perivascular sheating, subcapsular cataract and myopia. Systemic manifestations are absent in WGN.
  • Sequence similarities
    Belongs to the aggrecan/versican proteoglycan family.
    Contains 1 C-type lectin domain.
    Contains 2 EGF-like domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
    Contains 2 Link domains.
    Contains 1 Sushi (CCP/SCR) domain.
  • Developmental stage
    Disappears after the cartilage development.
  • Post-translational
    modifications
    Phosphorylation sites are present in the extracelllular medium.
  • Cellular localization
    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • Chondroitin sulfate proteoglycan 2 antibody
    • Chondroitin sulfate proteoglycan core protein 2 antibody
    • Chondroitin sulfate proteoglycan core protein, cartilage antibody
    • CSPG2 antibody
    • CSPG2_HUMAN antibody
    • ERVR antibody
    • GHAP antibody
    • Glial hyaluronate binding protein antibody
    • Glial hyaluronate-binding protein antibody
    • Large fibroblast proteoglycan antibody
    • PG-M antibody
    • PGM antibody
    • VCAN antibody
    • Versican antibody
    • Versican core protein antibody
    • Versican proteoglycan antibody
    • WGN 1 antibody
    • WGN antibody
    • WGN1 antibody
    see all

Images

  • Anti-Versican antibody (ab19345) at 1 µg/ml + Lysates prepared from human aorta.


    ab19345 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~70 kDa protein representing Versican from mouse cumulus oocyte complexes.

References

This product has been referenced in:
  • Suna G  et al. Extracellular Matrix Proteomics Reveals Interplay of Aggrecan and Aggrecanases in Vascular Remodeling of Stented Coronary Arteries. Circulation 137:166-183 (2018). Read more (PubMed: 29030347) »
  • Asano K  et al. Stromal Versican Regulates Tumor Growth by Promoting Angiogenesis. Sci Rep 7:17225 (2017). IHC-P ; Mouse . Read more (PubMed: 29222454) »
See all 36 Publications for this product

Customer reviews and Q&As

1-10 of 19 Abreviews or Q&A

Application
Western blot
Sample
Mouse Tissue lysate - whole (Heart)
Gel Running Conditions
Reduced Denaturing
Loading amount
8 µg
Specification
Heart
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Oct 04 2018

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (SKIN)
Permeabilization
Yes - PBST
Specification
SKIN
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Acetone

Abcam user community

Verified customer

Submitted Jan 23 2017

Application
Western blot
Sample
Human Tissue lysate - other (Aorta)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
5 µg
Treatment
PNGaseF
Specification
Aorta
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C

Dr. Javier Barallobre-Barreiro

Verified customer

Submitted Jun 24 2015

Question
Answer

Thank you for contacting Abcam regarding the anti-Versican antibody ab19345.

Upon further investigation on how to use this antibody in IHC, I would like to recommend the following publications for your reference. We do not routinely test the antibody in this application, but have received customer reports that it works well.

1.Pasquinelli G et al. Mesenchymal stem cell interaction with a non-woven hyaluronan-based scaffold suitable for tissue repair. J Anat 213:520-30 (2008).

2.Aga M et al. Specialized podosome- or invadopodia-like structures (PILS) for focal trabecular meshwork extracellular matrix turnover. Invest Ophthalmol Vis Sci 49:5353-65 (2008).


I know you have had some questions about the immunogen and specifically what form of the protein is being recognzied. We are not experts in this field, but the originator was able to provide the following details:

This product is against the neoepitope of versican generated by cleavage of the versican core protein by ATAMTS 1/4. "The proteases ADAMTS-1/4 have been shown to cleave versican V0 and V1 in the GAG{beta} domain in vitro and in human aortic intima in vivo. This cleavage releases a 70-kDa N-terminal fragment from V1, thus severing the matrix cross-linking properties of the N- and C-terminal globular domains."

The following references also help to explain this:

Russell DL et al. Processing and localization of ADAMTS-1 and proteolytic cleavage of versican during cumulus matrix expansion and ovulation. J Biol Chem 278:42330-9 (2003). PubMed: 12907688

Sandy JD et al. Versican V1 proteolysis in human aorta in vivo occurs at the Glu441-Ala442 bond, a site that is cleaved by recombinant ADAMTS-1 and ADAMTS-4. J Biol Chem 276:13372-8 (2001). PubMed: 11278559



I hope this information is helpful and clarifies any questions you have about this product. Please do not hesitate to contact us if we can be of any further assistance.

Read More

Answer

Thank you for contacting Abcam regarding ab13945.


I have contacted the originator of this antibody and unfortunately I do not have any additional information regarding its use as a total measure of versican in the cell, as it will only recognize the cleaved version. It may be useful to search the primary literature to learn more about how the detection of the cleaved protein translates into the analysis of total protein expression.


I do apologize that I am not able to provide more detailed information regarding your question. Please do not hesitate to contact me if you have any additional questions or concerns.

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Question
Answer

Vielen Dank für Ihren Anruf.



Leider habe ich eine schlechte Nachricht für Sie: Aggrecan hat insgesamt eine Übereinstimmung von 19%, es gibt eine Region zwischen AS 800-850 des menschlichen Proteins, welches eine hohe Übereinstimmung mit dem Ziegen Protein hat, nur haben wir leider keinen passenden Antikörper.

Versican und SOX5 weisen leider gar keine Übereinstimmung mit dem Ziegenprotein auf.

Da alle unsere Aggrecan,Versican und SOX5Antikörper gegen das humane Protein entwickelt worden sind, kannich Ihnen leider keinen Vorschlag machen.

Ich hoffe, dies hilft Ihnen trotzdem weiter. Bitte zögern Sie nicht, sich wieder bei uns zu melden, falls Sie weitere Fragen haben.

Read More

Answer

Thank you for contacting us.

Please find attached the WB protocol;

https://www.abcam.com/index.html?pageconfig=resource&rid=13045

Please find attached the link which actually explains the reason of band at ˜70-80 kDa. This is the cleavage product of versican. Please refer to fig 1.

Please be also advised that there are 5 isoforms of versican protein i.e. V0, V1, V2, V3, Vint. http://www.uniprot.org/uniprot/P13611
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Question
Answer

Thank you for contacting us. The immunogen for the antibody was CGG-DPEAAE. The CGG residues are part of the immunogen but not of the protein itself and the immunogen sequence matches to the isoform V1 of human versican protein,residues436-441. The anti-Versican antibody ab19345does not detect the full-length (266 kDa) protein, but only the 70 kDa N-terminal ADAMTS cleavage product of versican V1. The proteases ADAMTS-1/4 cleave versicanV1 in the GAG domain in vitro and in human aortic intima in vivo. This cleavage releases a 70 kDa N-terminal fragment from V1, thus severing the matrix cross-linking properties of the N- and C-terminal globular domains. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Answer

Thank you for your enquiry. Have you checked the references linked to the datasheet for ab19345? I've attached a PDF file of one of these references where ab19345 was used for IHC-P. I hope this helps. Please let me know if you continue to have trouble with this antibody.

Read More

Answer

Thanks for your reply. I am sorry to hear you have received less than the expected volume in the vial of ab19345 you received. If you have received the product within the last 6 months or so I would be happy to send you a replacement vial. If so, please let me know the purchase order number or Abcam order reference number associated with this order so I can process your request.

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1-10 of 19 Abreviews or Q&A

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