Recombinant
RabMAb

Anti-Versican antibody [EPR12277] (ab177480)

Overview

  • Product name
    Anti-Versican antibody [EPR12277]
    See all Versican primary antibodies
  • Description
    Rabbit monoclonal [EPR12277] to Versican
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Versican aa 3200-3300 (Cysteine residue). The exact sequence is proprietary.
    Database link: P13611

  • Positive control
    • WB: Fetal brain, fetal heart, rat and mouse hippocampal, mouse heart lysates; IHC-P: Human brain, Human bladder, mouse liver, Rat cerebrum tissues.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab177480 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100.
WB 1/1000 - 1/5000. Predicted molecular weight: 373 kDa.

Target

  • Function
    May play a role in intercellular signaling and in connecting cells with the extracellular matrix. May take part in the regulation of cell motility, growth and differentiation. Binds hyaluronic acid.
  • Tissue specificity
    Cerebral white matter and plasma. Isoform V0 and isoform V1 are expressed in normal brain, gliomas, medulloblastomas, schwannomas, neurofibromas, and meningiomas. Isoform V2 is restricted to normal brain and gliomas. Isoform V3 is found in all these tissues except medulloblastomas.
  • Involvement in disease
    Defects in VCAN are the cause of Wagner syndrome type 1 (WGN1) [MIM:143200]. WGN is a dominantly inherited vitreoretinopathy characterized by an optically empty vitreous cavity with fibrillary condensations and a preretinal avascular membrane. Other optical features include progressive chorioretinal atrophy, perivascular sheating, subcapsular cataract and myopia. Systemic manifestations are absent in WGN.
  • Sequence similarities
    Belongs to the aggrecan/versican proteoglycan family.
    Contains 1 C-type lectin domain.
    Contains 2 EGF-like domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
    Contains 2 Link domains.
    Contains 1 Sushi (CCP/SCR) domain.
  • Developmental stage
    Disappears after the cartilage development.
  • Post-translational
    modifications
    Phosphorylation sites are present in the extracelllular medium.
  • Cellular localization
    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • Chondroitin sulfate proteoglycan 2 antibody
    • Chondroitin sulfate proteoglycan core protein 2 antibody
    • Chondroitin sulfate proteoglycan core protein, cartilage antibody
    • CSPG2 antibody
    • CSPG2_HUMAN antibody
    • ERVR antibody
    • GHAP antibody
    • Glial hyaluronate binding protein antibody
    • Glial hyaluronate-binding protein antibody
    • Large fibroblast proteoglycan antibody
    • PG-M antibody
    • PGM antibody
    • VCAN antibody
    • Versican antibody
    • Versican core protein antibody
    • Versican proteoglycan antibody
    • WGN 1 antibody
    • WGN antibody
    • WGN1 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat cerebrum tissue sections labeling Versican with Purified ab177480 at 1:100 dilution (1.09 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylin was used as a counterstain.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human bladder cancer tissue sections labeling Versican with Purified ab177480 at 1:100 dilution (1.09 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylin was used as a counterstain.
  • All lanes : Anti-Versican antibody [EPR12277] (ab177480) at 0.1 µg/ml (purified)

    Lane 1 : Human fetal brain lysates
    Lane 2 : Mouse hippocampus lysates
    Lane 3 : Mouse heart lysates
    Lane 4 : Rat hippocampus lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 373 kDa



    Blocking and diluting buffer: 5% NFDM/TBST

    This antibody detects multi-bands due to the splicing variants and proteolytic fragments (PMID: 27683612, 24401530, 29222454, 19662655)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse liver tissue sections labeling Versican with Purified ab177480 at 1:100 dilution (1.09 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylin was used as a counterstain.
  • Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling Versican with unpurified ab177480 at 1/50 dilution.

  • All lanes : Anti-Versican antibody [EPR12277] (ab177480) at 1/1000 dilution (Unpurified)

    Lane 1 : Fetal brain lysate
    Lane 2 : Fetal heart lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 373 kDa

  • Unpurified ab177480 staining Versican in mouse cortex and hippocampus tissue section by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde, permeabilized with Triton X and blocked with 3% BSA for 1 hour at 20°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/200 in PBS + 2% BSA + Triton X) for 18 hours at 4°C. A HRP-conjugated goat anti-mouse IgG polyclonal (1/500) was used as the secondary antibody.

    See Abreview

References

This product has been referenced in:
  • Goffena J  et al. Elongator and codon bias regulate protein levels in mammalian peripheral neurons. Nat Commun 9:889 (2018). Read more (PubMed: 29497044) »
  • Mitsui Y  et al. Versican Promotes Tumor Progression, Metastasis and Predicts Poor Prognosis in Renal Carcinoma. Mol Cancer Res 15:884-895 (2017). Read more (PubMed: 28242813) »

See all 7 Publications for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Mouse brain, cortex)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate
Permeabilization
No
Specification
Mouse brain, cortex
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1.5% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Jul 28 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid buffer
Sample
Mouse Tissue sections (mouse cortex and hippocampus)
Specification
mouse cortex and hippocampus
Permeabilization
Yes - Triton X
Fixative
Paraformaldehyde
Username

Dr. Martin Broadstock

Verified customer

Submitted Feb 26 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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