Recombinant
RabMAb

Recombinant Anti-Versican antibody [EPR12277] (ab177480)

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Overview

  • Product name

    Anti-Versican antibody [EPR12277]
    See all Versican primary antibodies

  • Description

    Rabbit monoclonal [EPR12277] to Versican

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human Versican aa 3200-3300 (Cysteine residue). The exact sequence is proprietary.
    Database link: P13611

  • Positive control

    • WB: Fetal brain, fetal heart, rat and mouse hippocampal, mouse heart lysates; IHC-P: Human brain, Human bladder, mouse liver, Rat cerebrum tissues.

  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab177480 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/1000 - 1/5000. Predicted molecular weight: 373 kDa.

Target

  • Function

    May play a role in intercellular signaling and in connecting cells with the extracellular matrix. May take part in the regulation of cell motility, growth and differentiation. Binds hyaluronic acid.

  • Tissue specificity

    Cerebral white matter and plasma. Isoform V0 and isoform V1 are expressed in normal brain, gliomas, medulloblastomas, schwannomas, neurofibromas, and meningiomas. Isoform V2 is restricted to normal brain and gliomas. Isoform V3 is found in all these tissues except medulloblastomas.

  • Involvement in disease

    Defects in VCAN are the cause of Wagner syndrome type 1 (WGN1) [MIM:143200]. WGN is a dominantly inherited vitreoretinopathy characterized by an optically empty vitreous cavity with fibrillary condensations and a preretinal avascular membrane. Other optical features include progressive chorioretinal atrophy, perivascular sheating, subcapsular cataract and myopia. Systemic manifestations are absent in WGN.

  • Sequence similarities

    Belongs to the aggrecan/versican proteoglycan family.
    Contains 1 C-type lectin domain.
    Contains 2 EGF-like domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
    Contains 2 Link domains.
    Contains 1 Sushi (CCP/SCR) domain.

  • Developmental stage

    Disappears after the cartilage development.

  • Post-translational
    modifications

    Phosphorylation sites are present in the extracelllular medium.

  • Cellular localization

    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • Chondroitin sulfate proteoglycan 2 antibody
    • Chondroitin sulfate proteoglycan core protein 2 antibody
    • Chondroitin sulfate proteoglycan core protein, cartilage antibody
    • CSPG2 antibody
    • CSPG2_HUMAN antibody
    • ERVR antibody
    • GHAP antibody
    • Glial hyaluronate binding protein antibody
    • Glial hyaluronate-binding protein antibody
    • Large fibroblast proteoglycan antibody
    • PG-M antibody
    • PGM antibody
    • VCAN antibody
    • Versican antibody
    • Versican core protein antibody
    • Versican proteoglycan antibody
    • WGN 1 antibody
    • WGN antibody
    • WGN1 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Versican antibody [EPR12277] (ab177480)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Versican antibody [EPR12277] (ab177480)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat cerebrum tissue sections labeling Versican with Purified ab177480 at 1:100 dilution (1.09 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylin was used as a counterstain.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Versican antibody [EPR12277] (ab177480)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Versican antibody [EPR12277] (ab177480)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human bladder cancer tissue sections labeling Versican with Purified ab177480 at 1:100 dilution (1.09 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylin was used as a counterstain.
  • Western blot - Anti-Versican antibody [EPR12277] (ab177480)
    Western blot - Anti-Versican antibody [EPR12277] (ab177480)
    All lanes : Anti-Versican antibody [EPR12277] (ab177480) at 0.1 µg/ml (purified)

    Lane 1 : Human fetal brain lysates
    Lane 2 : Mouse hippocampus lysates
    Lane 3 : Mouse heart lysates
    Lane 4 : Rat hippocampus lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 373 kDa



    Blocking and diluting buffer: 5% NFDM/TBST

    This antibody detects multi-bands due to the splicing variants and proteolytic fragments (PMID: 27683612, 24401530, 29222454, 19662655)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Versican antibody [EPR12277] (ab177480)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Versican antibody [EPR12277] (ab177480)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse liver tissue sections labeling Versican with Purified ab177480 at 1:100 dilution (1.09 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylin was used as a counterstain.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Versican antibody [EPR12277] (ab177480)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Versican antibody [EPR12277] (ab177480)

    Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling Versican with unpurified ab177480 at 1/50 dilution.

  • Western blot - Anti-Versican antibody [EPR12277] (ab177480)
    Western blot - Anti-Versican antibody [EPR12277] (ab177480)
    All lanes : Anti-Versican antibody [EPR12277] (ab177480) at 1/1000 dilution (Unpurified)

    Lane 1 : Fetal brain lysate
    Lane 2 : Fetal heart lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 373 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Versican antibody [EPR12277] (ab177480)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Versican antibody [EPR12277] (ab177480)This image is courtesy of an Abreview submitted by Martin Broadstock

    Unpurified ab177480 staining Versican in mouse cortex and hippocampus tissue section by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde, permeabilized with Triton X and blocked with 3% BSA for 1 hour at 20°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/200 in PBS + 2% BSA + Triton X) for 18 hours at 4°C. A HRP-conjugated goat anti-mouse IgG polyclonal (1/500) was used as the secondary antibody.

    See Abreview

References

This product has been referenced in:

  • Song JY  et al. Glatiramer acetate persists at the injection site and draining lymph nodes via electrostatically-induced aggregation. J Control Release 293:36-47 (2019). Read more (PubMed: 30414463) »
  • Zhang Q  et al. Cancer-associated stroma significantly contributes to the mesenchymal subtype signature of serous ovarian cancer. Gynecol Oncol N/A:N/A (2018). Read more (PubMed: 30448260) »
See all 13 Publications for this product

Publishing research using ab177480? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-3 of 3 Abreviews or Q&A

Western blot abreview for Anti-Versican antibody [EPR12277]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Bos taurus Purified protein (Conditioned medium from vascular smooth muscle cel)
Gel Running Conditions
Reduced Denaturing (4-12% gradient)
Loading amount
550000 cells
Treatment
Chondroitinase ABC (1.5mU/µL) for 3 hrs
Specification
Conditioned medium from vascular smooth muscle cel
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

Submitted Oct 12 2018

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Versican antibody [EPR12277]

 Average
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Mouse brain, cortex)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate
Permeabilization
No
Specification
Mouse brain, cortex
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1.5% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

Submitted Jul 28 2017

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Versican antibody [EPR12277]

 Excellent
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid buffer
Sample
Mouse Tissue sections (mouse cortex and hippocampus)
Specification
mouse cortex and hippocampus
Permeabilization
Yes - Triton X
Fixative
Paraformaldehyde
Read More

Dr. Martin Broadstock

Verified customer

Submitted Feb 26 2015

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