Name: Anti-Versican antibody [EPR12277]
Brand: RabMAb
SKU: ab177480
Rating: 4 (3 reviews)

Product name

Anti-Versican antibody [EPR12277]
See all Versican primary antibodies
Description

Rabbit monoclonal [EPR12277] to Versican
Host species

Rabbit
Tested applications

Suitable for: IHC-P, WBmore details
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Synthetic peptide within Human Versican aa 3200-3300 (Cysteine residue). The exact sequence is proprietary.
Database link: P13611
Positive control
- WB: Fetal brain, fetal heart, rat and mouse hippocampal, mouse heart lysates; IHC-P: Human brain, Human bladder, mouse liver, Rat cerebrum tissues.
General notes

Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab^® patents
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.5% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR12277
Isotype

IgG
Research areas

Alternative Versions
- Anti-Versican antibody [EPR12277] - BSA and Azide free (ab240200)
Compatible Secondaries
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

Our Abpromise guarantee covers the use of ab177480 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
IHC-P		1/50 - 1/100.
WB		1/1000 - 1/5000. Predicted molecular weight: 373 kDa.

Function

May play a role in intercellular signaling and in connecting cells with the extracellular matrix. May take part in the regulation of cell motility, growth and differentiation. Binds hyaluronic acid.
Tissue specificity

Cerebral white matter and plasma. Isoform V0 and isoform V1 are expressed in normal brain, gliomas, medulloblastomas, schwannomas, neurofibromas, and meningiomas. Isoform V2 is restricted to normal brain and gliomas. Isoform V3 is found in all these tissues except medulloblastomas.
Involvement in disease

Defects in VCAN are the cause of Wagner syndrome type 1 (WGN1) [MIM:143200]. WGN is a dominantly inherited vitreoretinopathy characterized by an optically empty vitreous cavity with fibrillary condensations and a preretinal avascular membrane. Other optical features include progressive chorioretinal atrophy, perivascular sheating, subcapsular cataract and myopia. Systemic manifestations are absent in WGN.
Sequence similarities

Belongs to the aggrecan/versican proteoglycan family.
Contains 1 C-type lectin domain.
Contains 2 EGF-like domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
Contains 2 Link domains.
Contains 1 Sushi (CCP/SCR) domain.
Developmental stage

Disappears after the cartilage development.
Post-translational
modifications

Phosphorylation sites are present in the extracelllular medium.
Cellular localization

Secreted > extracellular space > extracellular matrix.
Target information above from: UniProt accession P13611 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 1462 Human
- Entrez Gene: 13003 Mouse
- Entrez Gene: 114122 Rat
- Omim: 118661 Human
- SwissProt: P13611 Human
- SwissProt: Q62059 Mouse
- SwissProt: Q9ERB4 Rat
- Unigene: 643801 Human
- Unigene: 715773 Human
- Unigene: 158700 Mouse
- Unigene: 410783 Mouse
- Unigene: 35666 Rat
see all
Alternative names
- Chondroitin sulfate proteoglycan 2 antibody
- Chondroitin sulfate proteoglycan core protein 2 antibody
- Chondroitin sulfate proteoglycan core protein, cartilage antibody
- CSPG2 antibody
- CSPG2_HUMAN antibody
- ERVR antibody
- GHAP antibody
- Glial hyaluronate binding protein antibody
- Glial hyaluronate-binding protein antibody
- Large fibroblast proteoglycan antibody
- PG-M antibody
- PGM antibody
- VCAN antibody
- Versican antibody
- Versican core protein antibody
- Versican proteoglycan antibody
- WGN 1 antibody
- WGN antibody
- WGN1 antibody
see all

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Versican antibody [EPR12277] (ab177480)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat cerebrum tissue sections labeling Versican with Purified ab177480 at 1:100 dilution (1.09 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylin was used as a counterstain.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Versican antibody [EPR12277] (ab177480)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human bladder cancer tissue sections labeling Versican with Purified ab177480 at 1:100 dilution (1.09 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylin was used as a counterstain.
Western blot - Anti-Versican antibody [EPR12277] (ab177480)

All lanes : Anti-Versican antibody [EPR12277] (ab177480) at 0.1 µg/ml (purified)

Lane 1 : Human fetal brain lysates
Lane 2 : Mouse hippocampus lysates
Lane 3 : Mouse heart lysates
Lane 4 : Rat hippocampus lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 373 kDa

Blocking and diluting buffer: 5% NFDM/TBST

This antibody detects multi-bands due to the splicing variants and proteolytic fragments (PMID: 27683612, 24401530, 29222454, 19662655)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Versican antibody [EPR12277] (ab177480)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse liver tissue sections labeling Versican with Purified ab177480 at 1:100 dilution (1.09 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylin was used as a counterstain.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Versican antibody [EPR12277] (ab177480)

Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling Versican with unpurified ab177480 at 1/50 dilution.
Western blot - Anti-Versican antibody [EPR12277] (ab177480)

All lanes : Anti-Versican antibody [EPR12277] (ab177480) at 1/1000 dilution (Unpurified)

Lane 1 : Fetal brain lysate
Lane 2 : Fetal heart lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 373 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Versican antibody [EPR12277] (ab177480)This image is courtesy of an Abreview submitted by Martin Broadstock

Unpurified ab177480 staining Versican in mouse cortex and hippocampus tissue section by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde, permeabilized with Triton X and blocked with 3% BSA for 1 hour at 20°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/200 in PBS + 2% BSA + Triton X) for 18 hours at 4°C. A HRP-conjugated goat anti-mouse IgG polyclonal (1/500) was used as the secondary antibody.
See Abreview

Western blot protocols
Immunohistochemistry protocols

Datasheet

This product has been referenced in:

Goffena J et al. Elongator and codon bias regulate protein levels in mammalian peripheral neurons. Nat Commun 9:889 (2018). Read more (PubMed: 29497044) »
Gaucherand L et al. Crosstalk Between T Lymphocytes and Lung Fibroblasts: Generation of a Hyaluronan-Enriched Extracellular Matrix Adhesive for Monocytes. J Cell Biochem 118:2118-2130 (2017). Read more (PubMed: 27982477) »

See all 8 Publications for this product

Publishing research using ab177480? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-3 of 3 Abreviews or Q&A

Application

Western blot

Sample

Bos taurus Purified protein (Conditioned medium from vascular smooth muscle cel)

Gel Running Conditions

Reduced Denaturing (4-12% gradient)

Loading amount

550000 cells

Treatment

Chondroitinase ABC (1.5mU/µL) for 3 hrs

Specification

Conditioned medium from vascular smooth muscle cel

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Oct 12 2018

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Mouse Tissue sections (Mouse brain, cortex)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: citrate

Permeabilization

Specification

Mouse brain, cortex

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1.5% · Temperature: 25°C

Fixative

Paraformaldehyde

Abcam user community

Verified customer

Submitted Jul 28 2017

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: Citric acid buffer

Sample

Mouse Tissue sections (mouse cortex and hippocampus)

Specification

mouse cortex and hippocampus

Permeabilization

Yes - Triton X

Fixative

Paraformaldehyde

Dr. Martin Broadstock

Verified customer

Submitted Feb 26 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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Anti-Versican antibody [EPR12277] (ab177480)

Overview

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References

This product has been referenced in:

Customer reviews and Q&As

Western blot abreview for Anti-Versican antibody [EPR12277]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Versican antibody [EPR12277]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Versican antibody [EPR12277]