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Synthetic peptide within Human Versican aa 3200-3300 (Cysteine residue). The exact sequence is proprietary.
Database link: P13611
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Our Abpromise guarantee covers the use of ab177480 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/100.|
|WB||1/1000 - 1/5000. Predicted molecular weight: 373 kDa.|
Blocking and diluting buffer: 5% NFDM/TBST
This antibody detects multi-bands due to the splicing variants and proteolytic fragments (PMID: 27683612, 24401530, 29222454, 19662655)
Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling Versican with unpurified ab177480 at 1/50 dilution.
Unpurified ab177480 staining Versican in mouse cortex and hippocampus tissue section by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde, permeabilized with Triton X and blocked with 3% BSA for 1 hour at 20°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/200 in PBS + 2% BSA + Triton X) for 18 hours at 4°C. A HRP-conjugated goat anti-mouse IgG polyclonal (1/500) was used as the secondary antibody.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"