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    versican-antibody-epr12277-ab177480.pdf

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Signal Transduction Cytoskeleton / ECM Extracellular Matrix ECM Proteins Aggrecan
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RecombinantRabMAb

Recombinant Anti-Versican antibody [EPR12277] (ab177480)

  • Datasheet
Reviews (3) Submit a question References (19)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Versican antibody [EPR12277] (ab177480)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Versican antibody [EPR12277] (ab177480)
  • Western blot - Anti-Versican antibody [EPR12277] (ab177480)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Versican antibody [EPR12277] (ab177480)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Versican antibody [EPR12277] (ab177480)
  • Western blot - Anti-Versican antibody [EPR12277] (ab177480)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Versican antibody [EPR12277] (ab177480)
  • Anti-Versican antibody [EPR12277] (ab177480)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12277] to Versican
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

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Recombinant Human Versican protein (ab152303)
Primary
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Anti-Lumican antibody [EPR8898(2)] (ab168348)

View more associated products

Overview

  • Product name

    Anti-Versican antibody [EPR12277]
    See all Versican primary antibodies
  • Description

    Rabbit monoclonal [EPR12277] to Versican
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Versican aa 3200-3300 (Cysteine residue). The exact sequence is proprietary.
    Database link: P13611

  • Positive control

    • WB: Fetal brain, fetal heart, rat and mouse hippocampal, mouse heart lysates; IHC-P: Human brain, Human bladder, mouse liver, Rat cerebrum tissues.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR12277
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Extracellular Matrix
    • ECM Proteins
    • Aggrecan
    • Signal Transduction
    • Adapters
    • Transmembrane

Associated products

  • Alternative Versions

    • Anti-Versican antibody [EPR12277] - BSA and Azide free (ab240200)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human Versican protein (ab152303)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab177480 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P (2)
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB (1)
1/1000 - 1/5000. Predicted molecular weight: 373 kDa.
Notes
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
1/1000 - 1/5000. Predicted molecular weight: 373 kDa.

Target

  • Function

    May play a role in intercellular signaling and in connecting cells with the extracellular matrix. May take part in the regulation of cell motility, growth and differentiation. Binds hyaluronic acid.
  • Tissue specificity

    Cerebral white matter and plasma. Isoform V0 and isoform V1 are expressed in normal brain, gliomas, medulloblastomas, schwannomas, neurofibromas, and meningiomas. Isoform V2 is restricted to normal brain and gliomas. Isoform V3 is found in all these tissues except medulloblastomas.
  • Involvement in disease

    Defects in VCAN are the cause of Wagner syndrome type 1 (WGN1) [MIM:143200]. WGN is a dominantly inherited vitreoretinopathy characterized by an optically empty vitreous cavity with fibrillary condensations and a preretinal avascular membrane. Other optical features include progressive chorioretinal atrophy, perivascular sheating, subcapsular cataract and myopia. Systemic manifestations are absent in WGN.
  • Sequence similarities

    Belongs to the aggrecan/versican proteoglycan family.
    Contains 1 C-type lectin domain.
    Contains 2 EGF-like domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
    Contains 2 Link domains.
    Contains 1 Sushi (CCP/SCR) domain.
  • Developmental stage

    Disappears after the cartilage development.
  • Post-translational
    modifications

    Phosphorylation sites are present in the extracelllular medium.
  • Cellular localization

    Secreted > extracellular space > extracellular matrix.
  • Target information above from: UniProt accession P13611 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 1462 Human
    • Entrez Gene: 13003 Mouse
    • Entrez Gene: 114122 Rat
    • Omim: 118661 Human
    • SwissProt: P13611 Human
    • SwissProt: Q62059 Mouse
    • SwissProt: Q9ERB4 Rat
    • Unigene: 643801 Human
    • Unigene: 715773 Human
    • Unigene: 158700 Mouse
    • Unigene: 410783 Mouse
    • Unigene: 35666 Rat
    see all
  • Alternative names

    • Chondroitin sulfate proteoglycan 2 antibody
    • Chondroitin sulfate proteoglycan core protein 2 antibody
    • Chondroitin sulfate proteoglycan core protein, cartilage antibody
    • CSPG2 antibody
    • CSPG2_HUMAN antibody
    • ERVR antibody
    • GHAP antibody
    • Glial hyaluronate binding protein antibody
    • Glial hyaluronate-binding protein antibody
    • Large fibroblast proteoglycan antibody
    • PG-M antibody
    • PGM antibody
    • VCAN antibody
    • Versican antibody
    • Versican core protein antibody
    • Versican proteoglycan antibody
    • WGN 1 antibody
    • WGN antibody
    • WGN1 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Versican antibody [EPR12277] (ab177480)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Versican antibody [EPR12277] (ab177480)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat cerebrum tissue sections labeling Versican with Purified ab177480 at 1:100 dilution (1.09 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylin was used as a counterstain.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Versican antibody [EPR12277] (ab177480)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Versican antibody [EPR12277] (ab177480)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human bladder cancer tissue sections labeling Versican with Purified ab177480 at 1:100 dilution (1.09 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylin was used as a counterstain.
  • Western blot - Anti-Versican antibody [EPR12277] (ab177480)
    Western blot - Anti-Versican antibody [EPR12277] (ab177480)
    All lanes : Anti-Versican antibody [EPR12277] (ab177480) at 0.1 µg/ml (purified)

    Lane 1 : Human hippocampus lysate
    Lane 2 : Mouse hippocampus lysates
    Lane 3 : Mouse heart lysates
    Lane 4 : Rat hippocampus lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 373 kDa



    Blocking and diluting buffer: 5% NFDM/TBST

    This antibody detects multi-bands due to the splicing variants and proteolytic fragments (PMID: 27683612, 24401530, 29222454, 19662655)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Versican antibody [EPR12277] (ab177480)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Versican antibody [EPR12277] (ab177480)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse liver tissue sections labeling Versican with Purified ab177480 at 1:100 dilution (1.09 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylin was used as a counterstain.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Versican antibody [EPR12277] (ab177480)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Versican antibody [EPR12277] (ab177480)

    Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling Versican with unpurified ab177480 at 1/50 dilution.

  • Western blot - Anti-Versican antibody [EPR12277] (ab177480)
    Western blot - Anti-Versican antibody [EPR12277] (ab177480)
    All lanes : Anti-Versican antibody [EPR12277] (ab177480) at 1/1000 dilution (Unpurified)

    Lane 1 : Fetal brain lysate
    Lane 2 : Fetal heart lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 373 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Versican antibody [EPR12277] (ab177480)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Versican antibody [EPR12277] (ab177480)This image is courtesy of an Abreview submitted by Martin Broadstock

    Unpurified ab177480 staining Versican in mouse cortex and hippocampus tissue section by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde, permeabilized with Triton X and blocked with 3% BSA for 1 hour at 20°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/200 in PBS + 2% BSA + Triton X) for 18 hours at 4°C. A HRP-conjugated goat anti-mouse IgG polyclonal (1/500) was used as the secondary antibody.

    See Abreview

  • Anti-Versican antibody [EPR12277] (ab177480)
    Anti-Versican antibody [EPR12277] (ab177480)

Protocols

  • Western blot protocols
  • Immunohistochemistry protocols

Click here to view the general protocols

Datasheets and documents

  • Datasheet download

    Download

References (19)

Publishing research using ab177480? Please let us know so that we can cite the reference in this datasheet.

ab177480 has been referenced in 19 publications.

  • Egerbacher M  et al. Stress-deprivation induces an up-regulation of versican and connexin-43 mRNA and protein synthesis and increased ADAMTS-1 production in tendon cells in situ. Connect Tissue Res N/A:1-10 (2021). PubMed: 33467936
  • Mutoji KN  et al. Extracellular Matrix Deposition and Remodeling after Corneal Alkali Burn in Mice. Int J Mol Sci 22:N/A (2021). PubMed: 34071909
  • Dai J  et al. TMT-labeling Proteomics of Papillary Thyroid Carcinoma Reveal Invasive Biomarkers. J Cancer 11:6122-6132 (2020). PubMed: 32922552
  • Yang L  et al. Up-regulation of EMT-related gene VCAN by NPM1 mutant-driven TGF-ß/cPML signalling promotes leukemia cell invasion. J Cancer 10:6570-6583 (2019). PubMed: 31777586
  • Virga J  et al. Extracellular matrix differences in glioblastoma patients with different prognoses. Oncol Lett 17:797-806 (2019). PubMed: 30655832
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-3 of 3 Abreviews or Q&A

Western blot abreview for Anti-Versican antibody [EPR12277]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Bos taurus Purified protein (Conditioned medium from vascular smooth muscle cel)
Gel Running Conditions
Reduced Denaturing (4-12% gradient)
Loading amount
550000 cells
Treatment
Chondroitinase ABC (1.5mU/µL) for 3 hrs
Specification
Conditioned medium from vascular smooth muscle cel
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Oct 12 2018

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Versican antibody [EPR12277]

Average
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Mouse brain, cortex)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate
Permeabilization
No
Specification
Mouse brain, cortex
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1.5% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jul 28 2017

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Versican antibody [EPR12277]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid buffer
Sample
Mouse Tissue sections (mouse cortex and hippocampus)
Specification
mouse cortex and hippocampus
Permeabilization
Yes - Triton X
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Dr. Martin Broadstock

Verified customer

Submitted Feb 26 2015

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