Product nameAnti-Versican antibody [EPR23374-21] - BSA and Azide free
See all Versican primary antibodies
DescriptionRabbit monoclonal [EPR23374-21] to Versican - BSA and Azide free
Tested applicationsSuitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-Fr,IHC-P or IP
Species reactivityReacts with: Mouse, Rat, Human
Recombinant fragment within Human Versican aa 3050 to the C-terminus. The exact sequence is proprietary.
Database link: P13611
- WB: Mouse brain, Human brain, Rat brain lysates.
ab272223 is the carrier-free version of ab270444. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab272223 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.??
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferConstituent: PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab272223 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Detects a band of approximately 370 kDa (predicted molecular weight: 373 kDa).|
FunctionMay play a role in intercellular signaling and in connecting cells with the extracellular matrix. May take part in the regulation of cell motility, growth and differentiation. Binds hyaluronic acid.
Tissue specificityCerebral white matter and plasma. Isoform V0 and isoform V1 are expressed in normal brain, gliomas, medulloblastomas, schwannomas, neurofibromas, and meningiomas. Isoform V2 is restricted to normal brain and gliomas. Isoform V3 is found in all these tissues except medulloblastomas.
Involvement in diseaseDefects in VCAN are the cause of Wagner syndrome type 1 (WGN1) [MIM:143200]. WGN is a dominantly inherited vitreoretinopathy characterized by an optically empty vitreous cavity with fibrillary condensations and a preretinal avascular membrane. Other optical features include progressive chorioretinal atrophy, perivascular sheating, subcapsular cataract and myopia. Systemic manifestations are absent in WGN.
Sequence similaritiesBelongs to the aggrecan/versican proteoglycan family.
Contains 1 C-type lectin domain.
Contains 2 EGF-like domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
Contains 2 Link domains.
Contains 1 Sushi (CCP/SCR) domain.
Developmental stageDisappears after the cartilage development.
modificationsPhosphorylation sites are present in the extracelllular medium.
Cellular localizationSecreted > extracellular space > extracellular matrix.
- Information by UniProt
- Chondroitin sulfate proteoglycan 2 antibody
- Chondroitin sulfate proteoglycan core protein 2 antibody
- Chondroitin sulfate proteoglycan core protein, cartilage antibody
All lanes : Anti-Versican antibody [EPR23374-21] (ab270444) at 1/1000 dilution
Lane 1 : Mouse brain tissue lysate
Lane 2 : Human brain tissue lysate
Lane 3 : MDA-MB-231 (human breast adenocarcinoma epithelial cell) whole cell lysate
Lane 4 : MeWo (human malignant melanoma fibroblast) whole cell lysate
Lane 5 : Rat brain tissue lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 373 kDa
Observed band size: 370 kDa why is the actual band size different from the predicted?
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Negative control: MDA-MB-231(PMID:29222454); MeWo(PMID:12972299).
Exposure time: 3 minutes.
This data was developed using the same antibody clone in a different buffer formulation containg PBS, glycerol, BSA and sodium azide (ab270444).
ab272223 has not yet been referenced specifically in any publications.