General vesicular transport factor required for intercisternal transport in the Golgi stack; it is required for transcytotic fusion and/or subsequent binding of the vesicles to the target membrane. May well act as a vesicular anchor by interacting with the target membrane and holding the vesicular and target membranes in proximity.
Belongs to the VDP/USO1/EDE1 family. Contains 10 ARM repeats.
Composed of a globular head, an elongated tail (coiled-coil) and a highly acidic C-terminal domain.
Phosphorylated in a cell cycle-specific manner; phosphorylated in interphase but not in mitotic cells. Dephosphorylated protein associates with the Golgi membrane; phosphorylation promotes dissociation.
Cytoplasm > cytosol. Golgi apparatus membrane. Recycles between the cytosol and the Golgi apparatus during interphase. During interphase, the phosphorylated form is found exclusively in cytosol; the unphosphorylated form is associated with Golgi apparatus membranes.
Western blot - Anti-Vesicle docking protein p115 antibody (ab184014)
All lanes : Anti-Vesicle docking protein p115 antibody (ab184014) at 1/1000 dilution
Lane 1 : 293T whole cell lysate Lane 2 : A431 whole cell lysate Lane 3 : HeLa whole cell lysate Lane 4 : HepG2 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 108 kDa
7.5% SDS PAGE
Immunocytochemistry/ Immunofluorescence - Anti-Vesicle docking protein p115 antibody (ab184014)
Immunocytochemical analysis of methanol fixed A431 cells labeling Vesicle docking protein p115 at 1/500 dilution (green) and counterstained with Hoechst 33342. Cells were fixed in 100% methanol for 5 mins.
Zhong B et al. Hsp20 Protects against Oxygen-Glucose Deprivation/Reperfusion-Induced Golgi Fragmentation and Apoptosis through Fas/FasL Pathway. Oxid Med Cell Longev2015:606934 (2015).
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