The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC - Wholemount
Use at an assay dependent concentration. Fix Tissue overnight in Zamboni fixative, then carry out 3 x 10min washes in DMSO and 3 x 10min washes in PBS. Tissue was then stored in PBS and Azide until being dissected and stained.
Use at an assay dependent dilution. Predicted molecular weight: 59 kDa.
Involved in acetylcholine transport into synaptic vesicles.
Peripheral and central cholinergic nervous systems.
Belongs to the major facilitator superfamily. Vesicular transporter family.