Anti-Vesicular Acetylcholine Transporter antibody (ab31544)

Overview

  • Product name
    Anti-Vesicular Acetylcholine Transporter antibody
  • Description
    Sheep polyclonal to Vesicular Acetylcholine Transporter
  • Host species
    Sheep
  • Specificity
    Ab31544 is specific to vesicular acetylcholine transporter.
  • Tested applications
    Suitable for: IHC - Wholemount, WBmore details
  • Species reactivity
    Reacts with: Rat, Guinea pig
    Predicted to work with: Mouse, Dog, Human, Chimpanzee, Macaque monkey
  • Immunogen

    Synthetic peptide:

    C-TRSRSERDVLLDEPPQGLYDAVRLRE

    , corresponding to amino acids 475-500 of Rat Vesicular Acetylcholine Transporter conjugated to Blue carrier protein.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab31544 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC - Wholemount Use at an assay dependent concentration. Fix Tissue overnight in Zamboni fixative, then carry out 3 x 10min washes in DMSO and 3 x 10min washes in PBS. Tissue was then stored in PBS and Azide until being dissected and stained.
WB Use at an assay dependent dilution. Predicted molecular weight: 59 kDa.

Target

References

ab31544 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
Western blot
Sample
Human Tissue lysate - whole (Lung)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Specification
Lung
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Dr. Safa Azar

Verified customer

Submitted Sep 13 2018

Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (parasympathetic nerve fibres)
Specification
parasympathetic nerve fibres
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
Fixative
Acetone

Abcam user community

Verified customer

Submitted Aug 28 2018

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Spinal cord)
Antigen retrieval step
None
Permeabilization
Yes - 0.1% Tween 20
Specification
Spinal cord
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Paraformaldehyde

Mrs. Teresa Garrett

Verified customer

Submitted Oct 14 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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