Overview

  • Product name

    Anti-VGCNL1 antibody
  • Description

    Mouse polyclonal to VGCNL1
  • Host species

    Mouse
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment: QQQLLSPTLS DRGGSRQDAA DAGKPQRKFG QWRLPSAPKP ISHSVSSVNL RFGGRTTMKS VVCKMNPMTD AASCGSEVKK WWTRQLTVES DESGDDLLDI, corresponding to amino acids 1639-1738 of Human VGCNL1

  • General notes


    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Constituents: Whole serum, 50% Glycerol
  • Purity

    Whole antiserum
  • Primary antibody notes

    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab43631 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 200 kDa.

This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.

Target

  • Function

    Voltage-independent, cation-nonselective channel which is permeable to sodium, potassium and calcium ions. Responsible for the background sodium ion leak current in neurons and controls neuronal excitability. Activated either by neuropeptides substance P or neurotensin. Required for normal respiratory rhythm and neonatal survival.
  • Involvement in disease

    Hypotonia, infantile, with psychomotor retardation and characteristic facies 1
    Congenital contractures of the limbs and face, hypotonia, and developmental delay
  • Sequence similarities

    Belongs to the cation-nonselective channel family.
  • Domain

    Each of the four internal repeats contains five hydrophobic transmembrane segments (S1, S2, S3, S5, S6) and one positively charged transmembrane segment (S4). S4 segments probably represent the voltage-sensor and are characterized by a series of positively charged amino acids at every third position.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • A530023G15Rik antibody
    • bA430M15.1 antibody
    • CanIon antibody
    • Canlon antibody
    • FLJ23913 antibody
    • FLJ44659 antibody
    • FLJ44764 antibody
    • Four repeat voltage gated ion channel antibody
    • MGC74524 antibody
    • Nalcn antibody
    • NALCN_HUMAN antibody
    • Putative 4 repeat voltage gated ion channel antibody
    • Sodium leak channel non-selective protein antibody
    • sodium leak channel, non selective antibody
    • Vgcnl1 antibody
    • VGCNL1 protein antibody
    • voltage gated channel like 1 antibody
    • Voltage gated channel-like protein 1 antibody
    see all

References

ab43631 has not yet been referenced specifically in any publications.

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