Key features and details
- Rabbit polyclonal to VGF
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-VGF antibody
See all VGF primary antibodies
DescriptionRabbit polyclonal to VGF
SpecificityThe immunogen is after amino acid 577 of O15240, so the antibody should only cross-react with full-length VGF and none of the processed peptides.
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
17 amino acid peptide near the carboxy terminus of the Human VGF.
- Human Brain Cortex tissue; Human Brain tissue lysate.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.02% Sodium azide
Constituent: 0.99% PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab115609 in the following tested applications.
|WB||Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 67 kDa.|
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionMay be involved in the regulation of cell-cell interactions or in synatogenesis during the maturation of the nervous system.
NERP peptides are involved in the control of body fluid homeostasis by regulating vasopressin release.
Tissue specificityCentral and peripheral nervous systems, synthesized exclusively in neuronal and neuroendocrine cells.
modificationsMultiple peptides are derived from VGF, with activities in synaptic plasticity, antidepression, penile erection, autonomic activation, and increases in energy expenditure.
Cellular localizationSecreted. Cytoplasmic vesicle > secretory vesicle. Stored in secretory vesicles and then secreted, NERP peptides colocalize with vasopressin in the storage granules of hypothalamus.
- Information by UniProt
- NERP-1 antibody
- NERP-2 antibody
- Nerve growth factor inducible antibody
ab115609, at 5µg/ml, staining VGF in Formalin-fixed, Paraffin-embedded Human Brain cortex tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Lane 1 : Anti-VGF antibody (ab115609) at 0.5 µg/ml
Lane 2 : Anti-VGF antibody (ab115609) at 1 µg/ml
All lanes : Human Brain tissue lysate
Predicted band size: 67 kDa
ab115609 has been referenced in 1 publication.
- Marwitz S et al. Epigenetic modifications of the VGF gene in human non-small cell lung cancer tissues pave the way towards enhanced expression. Clin Epigenetics 9:123 (2017). PubMed: 29209432