Anti-VGF antibody (ab69989)
Key features and details
- Rabbit polyclonal to VGF
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-VGF antibody
See all VGF primary antibodies -
Description
Rabbit polyclonal to VGF -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
17 amino acid peptide near the carboxy terminus of the human VGF (GenBank accession no. NP_003369).
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Positive control
- WB: Human brain tissue lysate. IHC-P: Human brain tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at +4°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab69989 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P | (1) |
Use a concentration of 5 µg/ml.
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WB | (2) |
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 67 kDa (predicted molecular weight: 67 kDa).
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Notes |
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IHC-P
Use a concentration of 5 µg/ml. |
WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 67 kDa (predicted molecular weight: 67 kDa). |
Target
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Function
May be involved in the regulation of cell-cell interactions or in synatogenesis during the maturation of the nervous system.
NERP peptides are involved in the control of body fluid homeostasis by regulating vasopressin release. -
Tissue specificity
Central and peripheral nervous systems, synthesized exclusively in neuronal and neuroendocrine cells. -
Post-translational
modificationsMultiple peptides are derived from VGF, with activities in synaptic plasticity, antidepression, penile erection, autonomic activation, and increases in energy expenditure. -
Cellular localization
Secreted. Cytoplasmic vesicle > secretory vesicle. Stored in secretory vesicles and then secreted, NERP peptides colocalize with vasopressin in the storage granules of hypothalamus. - Information by UniProt
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Database links
- Entrez Gene: 7425 Human
- Omim: 602186 Human
- SwissProt: O15240 Human
- Unigene: 587325 Human
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Alternative names
- NERP-1 antibody
- NERP-2 antibody
- Nerve growth factor inducible antibody
see all
Images
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Lane 1 : Anti-VGF antibody (ab69989) at 0.5 µg/ml
Lane 2 : Anti-VGF antibody (ab69989) at 1 µg/ml
All lanes : Human brain tissue lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 67 kDa
Observed band size: 67 kDa -
ab69989 at 5 µg/ml staining VGF in human brain tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (9)
ab69989 has been referenced in 9 publications.
- Noda Y et al. Identification of VGF nerve growth factor inducible-producing cells in human spinal cords and expression change in patients with amyotrophic lateral sclerosis. Int J Med Sci 17:480-489 (2020). PubMed: 32174778
- Hwang I et al. CIC is a critical regulator of neuronal differentiation. JCI Insight 5:N/A (2020). PubMed: 32229723
- Higginbotham L et al. Integrated proteomics reveals brain-based cerebrospinal fluid biomarkers in asymptomatic and symptomatic Alzheimer's disease. Sci Adv 6:N/A (2020). PubMed: 33087358
- Bai B et al. Deep Multilayer Brain Proteomics Identifies Molecular Networks in Alzheimer's Disease Progression. Neuron 105:975-991.e7 (2020). PubMed: 31926610
- Alrawashdeh W et al. Perineural invasion in pancreatic cancer: proteomic analysis and in vitro modelling. Mol Oncol 13:1075-1091 (2019). PubMed: 30690892
- Thompson SM et al. Heat Stress and Thermal Ablation Induce Local Expression of Nerve Growth Factor Inducible (VGF) in Hepatocytes and Hepatocellular Carcinoma: Preclinical and Clinical Studies. Gene Expr 19:37-47 (2018). PubMed: 29973305
- Shiraishi A et al. Generation of thalamic neurons from mouse embryonic stem cells. Development 144:1211-1220 (2017). PubMed: 28219951
- Annaratone L et al. Search for neuro-endocrine markers (chromogranin A, synaptophysin and VGF) in breast cancers. An integrated approach using immunohistochemistry and gene expression profiling. Endocr Pathol 25:219-28 (2014). WB, IHC-P ; Human . PubMed: 24277232
- Dmitrieff EF et al. Chronic hyperoxia alters the expression of neurotrophic factors in the carotid body of neonatal rats. Respir Physiol Neurobiol 175:220-7 (2011). PubMed: 21094282