The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 20 µg/ml.
Use a concentration of 5 µg/ml.
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 67 kDa (predicted molecular weight: 67 kDa).
May be involved in the regulation of cell-cell interactions or in synatogenesis during the maturation of the nervous system. NERP peptides are involved in the control of body fluid homeostasis by regulating vasopressin release.
Central and peripheral nervous systems, synthesized exclusively in neuronal and neuroendocrine cells.
Multiple peptides are derived from VGF, with activities in synaptic plasticity, antidepression, penile erection, autonomic activation, and increases in energy expenditure.
Secreted. Cytoplasmic vesicle > secretory vesicle. Stored in secretory vesicles and then secreted, NERP peptides colocalize with vasopressin in the storage granules of hypothalamus.
ICC/IF image of ab69989 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab69989, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Annaratone L et al. Search for neuro-endocrine markers (chromogranin A, synaptophysin and VGF) in breast cancers. An integrated approach using immunohistochemistry and gene expression profiling. Endocr Pathol25:219-28 (2014).
Read more (PubMed: 24277232) »
Dmitrieff EF et al. Chronic hyperoxia alters the expression of neurotrophic factors in the carotid body of neonatal rats. Respir Physiol Neurobiol175:220-7 (2011).
Read more (PubMed: 21094282) »