Overview

  • Product name

  • Description

    Rabbit polyclonal to VGF
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, ELISA, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide derived from a internal sequence of human VGF.

  • Positive control

    • Human lung carcinoma. Extracts from RAW264.7 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab74140 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB 1/500 - 1/1000. Predicted molecular weight: 67 kDa.
ELISA 1/10000.
IHC-P 1/50 - 1/100.

Target

  • Function

    May be involved in the regulation of cell-cell interactions or in synatogenesis during the maturation of the nervous system.
    NERP peptides are involved in the control of body fluid homeostasis by regulating vasopressin release.
  • Tissue specificity

    Central and peripheral nervous systems, synthesized exclusively in neuronal and neuroendocrine cells.
  • Post-translational
    modifications

    Multiple peptides are derived from VGF, with activities in synaptic plasticity, antidepression, penile erection, autonomic activation, and increases in energy expenditure.
  • Cellular localization

    Secreted. Cytoplasmic vesicle > secretory vesicle. Stored in secretory vesicles and then secreted, NERP peptides colocalize with vasopressin in the storage granules of hypothalamus.
  • Information by UniProt
  • Database links

  • Alternative names

    • NERP-1 antibody
    • NERP-2 antibody
    • Nerve growth factor inducible antibody
    • Neuroendocrine regulatory peptide-2 antibody
    • Neurosecretory protein VGF antibody
    • VGF antibody
    • VGF_HUMAN antibody
    see all

Images

  • All lanes : Anti-VGF antibody (ab74140) at 1/500 dilution

    Lane 1 : Extracts from RAW264.7 cells
    Lane 2 : Extracts from RAW264.7 cells with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.

    Predicted band size: 67 kDa
    Observed band size: 67 kDa

  • Immunohistochemical analysis of VGF in paraffin embedded human lung carcinoma tissue using ab74140, at a 1/50 dilution, in the presence (right panel) or absence (left panel) of immunising peptide.
  • ICC/IF image of ab74140 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab74140, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:

  • Asrari N  et al. Antidepressant effects of aqueous extract of saffron and its effects on CREB, P-CREB, BDNF, and VGF proteins in rat cerebellum. J Pharmacopuncture 21:35-40 (2018). Read more (PubMed: 30151303) »
  • Razavi BM  et al. Study of the Role of CREB, BDNF, and VGF Neuropeptide in Long Term Antidepressant Activity of Crocin in the Rat Cerebellum. Iran J Pharm Res 16:1452-1462 (2017). Read more (PubMed: 29552054) »
See all 3 Publications for this product

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